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    rpsA 30S ribosomal subunit protein S1 [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 945536, updated on 3-Dec-2024

    Summary

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    Official Symbol
    rpsA
    Official Full Name
    30S ribosomal subunit protein S1
    Primary source
    ECOCYC:EG10900
    Locus tag
    b0911
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK0902; ssyF
    Summary
    HT_Cmplx22_Cyt: [More information is available at EcoGene: EG10900]. The S1 protein is essential in Escherichia coli; it is a component of the ribosome and is likely required for translation of most mRNAs . [More information is available at EcoCyc: EG10900].
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    Genomic context

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    Sequence:
    NC_000913.3 (961995..963668)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene periplasmic protease YcaL Neighboring gene cytidylate kinase Neighboring gene integration host factor subunit beta Neighboring gene metallo-beta-lactamase superfamily inner membrane protein YcaI

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Ribosome-messenger recognition in the absence of the Shine-Dalgarno interactions. Tzareva NV, et al. FEBS Lett, 1994 Jan 10. PMID 8287975
    2. Ribosomal protein S1 promotes transcriptional cycling. Sukhodolets MV, et al. RNA, 2006 Aug. PMID 16775305, Free PMC Article
    3. AU-rich sequences within 5' untranslated leaders enhance translation and stabilize mRNA in Escherichia coli. Komarova AV, et al. J Bacteriol, 2005 Feb. PMID 15687198, Free PMC Article
    4. Ribosomal protein S1 binds mRNA and tmRNA similarly but plays distinct roles in translation of these molecules. McGinness KE, et al. Proc Natl Acad Sci U S A, 2004 Sep 14. PMID 15340139, Free PMC Article
    5. A key role for the mRNA leader structure in translational control of ribosomal protein S1 synthesis in gamma-proteobacteria. Tchufistova LS, et al. Nucleic Acids Res, 2003 Dec 1. PMID 14627832, Free PMC Article

    See all (102) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Structural insights into the binding of bS1 to the ribosome.
      Title: Structural insights into the binding of bS1 to the ribosome.
    2. An RNA pseudoknot is essential for standby-mediated translation of the tisB toxin mRNA in Escherichia coli.
      Title: An RNA pseudoknot is essential for standby-mediated translation of the tisB toxin mRNA in Escherichia coli.
    3. The ability of S1 to unfold the RNA is inversely correlated with the structural stability of the pseudoknot. These mechanistic insights delineate the scope and limitations of S1-chaperoned unfolding of structured RNAs.
      Title: Protein unties the pseudoknot: S1-mediated unfolding of RNA higher order structure.
    4. Single-particle cryo-electron microscopy structures of hibernating 70S and 100S particles isolated from stationary-phase E. coli reveal the presence of deacylated E-site transfer RNA and ribosomal protein bS1. A compact conformation of bS1 sequesters the anti-Shine-Dalgarno sequence of the 16S ribosomal RNA (rRNA), thereby inhibiting translation initiation.
      Title: Structure of a hibernating 100S ribosome reveals an inactive conformation of the ribosomal protein S1.
    5. r-protein S1 endows the 30S with an RNA chaperone activity that is essential for the docking and the unfolding of structured mRNAs, and for the correct positioning of the initiation codon inside the decoding channel
      Title: Escherichia coli ribosomal protein S1 unfolds structured mRNAs onto the ribosome for active translation initiation.
    6. Ribosomal protein S1 unwinds double-stranded RNA in multiple steps.
      Title: Ribosomal protein S1 unwinds double-stranded RNA in multiple steps.
    7. N-terminus verified by Edman degradation on complete protein
      Title: Protein factors associated with the SsrA.SmpB tagging and ribosome rescue complex.
    8. The last S1 motif is dispensable for translation and autoregulation: the defects seen with ssyF29 cells reflect an IS10R-mediated destabilization of the rpsA mRNA
      Title: The deleterious effect of an insertion sequence removing the last twenty percent of the essential Escherichia coli rpsA gene is due to mRNA destabilization, not protein truncation.
    9. S1 binding has a weak but detectable effect on the secondary structure of DsrA or rpoS mRNA.
      Title: Interactions of ribosomal protein S1 with DsrA and rpoS mRNA.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables RNA binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables RNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables mRNA binding IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables mRNA binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables single-stranded RNA binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables structural constituent of ribosome IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables structural constituent of ribosome IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables structural constituent of ribosome IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    acts_upstream_of_or_within RNA secondary structure unwinding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in cytoplasmic translation NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    acts_upstream_of_or_within negative regulation of cytoplasmic translation IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    acts_upstream_of_or_within negative regulation of cytoplasmic translation IEP
    Inferred from Expression Pattern
    more info
    		 PubMed 
    acts_upstream_of_or_within positive regulation of cytoplasmic translation IEP
    Inferred from Expression Pattern
    more info
    		 PubMed 
    involved_in ribosomal small subunit assembly IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in translation IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in translation IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within translation IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in cytoplasm HDA PubMed 
    located_in cytoplasm IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  
    part_of cytosolic small ribosomal subunit IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    part_of cytosolic small ribosomal subunit IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    part_of cytosolic small ribosomal subunit IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    located_in membrane HDA PubMed 
    part_of ribonucleoprotein complex IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in ribosome IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

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    Preferred Names
    30S ribosomal subunit protein S1

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      961995..963668
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_415431.1 30S ribosomal subunit protein S1 [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_415431.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0AAN3GYA1
      Conserved Domains (1) summary
      PRK06299
      Location:1 → 557
      rpsA; 30S ribosomal protein S1; Reviewed
    Nucleotide Protein
    Heading Accession and Version

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