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    otsA trehalose-6-phosphate synthase [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 946405, updated on 17-Dec-2024

    Summary

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    Official Symbol
    otsA
    Official Full Name
    trehalose-6-phosphate synthase
    Primary source
    ECOCYC:EG11751
    Locus tag
    b1896
    See related
    ASAP:ABE-0006318
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK1895
    Summary
    Under conditions of high osmolarity, Escherichia coli synthesizes high concentrations of internal trehalose. [More information is available at EcoCyc: EG11751].
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    Genomic context

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    Sequence:
    NC_000913.3 (1980188..1981612, complement)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene IS1 family protein InsB Neighboring gene IS1 family protein InsA Neighboring gene universal stress protein C Neighboring gene trehalose-6-phosphate phosphatase Neighboring gene arabinose ABC transporter membrane subunit Neighboring gene arabinose ABC transporter ATP binding subunit

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Trehalose protects Escherichia coli against carbon stress manifested by protein acetylation and aggregation. Moruno Algara M, et al. Mol Microbiol, 2019 Sep. PMID 31162854
    2. Enhanced trehalose production improves growth of Escherichia coli under osmotic stress. Purvis JE, et al. Appl Environ Microbiol, 2005 Jul. PMID 16000787, Free PMC Article
    3. The donor subsite of trehalose-6-phosphate synthase: binary complexes with UDP-glucose and UDP-2-deoxy-2-fluoro-glucose at 2 A resolution. Gibson RP, et al. J Biol Chem, 2004 Jan 16. PMID 14570926
    4. Insights into trehalose synthesis provided by the structure of the retaining glucosyltransferase OtsA. Gibson RP, et al. Chem Biol, 2002 Dec. PMID 12498887
    5. Characterization of Escherichia coli OtsA, a trehalose-6-phosphate synthase from glycosyltransferase family 20. Gibson RP, et al. Acta Crystallogr D Biol Crystallogr, 2002 Feb. PMID 11807274

    See all (24) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Deletion of otsA resulted in lack of trehalose synthesis and leaded to increased protein aggregation during the stationary phase.
      Title: Trehalose protects Escherichia coli against carbon stress manifested by protein acetylation and aggregation.
    2. N-terminus verified by Edman degradation on mature peptide
      Title: Analysis of the otsBA operon for osmoregulatory trehalose synthesis in Escherichia coli and homology of the OtsA and OtsB proteins to the yeast trehalose-6-phosphate synthase/phosphatase complex.
    Submit: New GeneRIF Correction

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables alpha,alpha-trehalose-phosphate synthase (UDP-forming) activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables alpha,alpha-trehalose-phosphate synthase (UDP-forming) activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables glycosyltransferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables hexosyltransferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    acts_upstream_of_or_within DNA damage response IEP
    Inferred from Expression Pattern
    more info
    		 PubMed 
    acts_upstream_of_or_within response to osmotic stress IEP
    Inferred from Expression Pattern
    more info
    		 PubMed 
    acts_upstream_of_or_within response to osmotic stress IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within response to stress IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in trehalose biosynthetic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within trehalose biosynthetic process IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within trehalose metabolism in response to cold stress IEP
    Inferred from Expression Pattern
    more info
    		 PubMed 
    acts_upstream_of_or_within trehalose metabolism in response to cold stress IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    trehalose-6-phosphate synthase
    NP_416410.1

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      1980188..1981612 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_416410.1 trehalose-6-phosphate synthase [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_416410.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A5D8MWE0, A0AAN5UE80
      Conserved Domains (1) summary
      PRK10117
      Location:1 → 474
      PRK10117; trehalose-6-phosphate synthase; Provisional
    Nucleotide Protein
    Heading Accession and Version

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