Trim63 tripartite motif-containing 63 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Trim63provided by MGI
Official Full Name: tripartite motif-containing 63provided by MGI
Primary source: MGI:MGI:2447992
See related: Ensembl:ENSMUSG00000028834 AllianceGenome:MGI:2447992
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: RF1; MuRF1; Rnf28
Summary: Enables ubiquitin protein ligase activity. Involved in protein monoubiquitination and response to denervation involved in regulation of muscle adaptation. Acts upstream of or within several processes, including cellular response to dexamethasone stimulus; myotube cell development; and proteasome-mediated ubiquitin-dependent protein catabolic process. Part of Cul4-RING E3 ubiquitin ligase complex. Is expressed in embryo and heart. Orthologous to human TRIM63 (tripartite motif containing 63). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Biased expression in heart adult (RPKM 54.2) and mammary gland adult (RPKM 5.3) See more
Orthologs: human all
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Genomic context

Location:: 4 D2.3; 4 66.58 cM

Exon count:: 9

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	4	NC_000070.7 (134042431..134056940)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	4	NC_000070.6 (134315120..134329629)

Chromosome 4 - NC_000070.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Acute exercise modulates Trim63 and Bmal1 in the skeletal muscle of IL-10 knockout mice.
Title: Acute exercise modulates Trim63 and Bmal1 in the skeletal muscle of IL-10 knockout mice.
Blocking muscle wasting via deletion of the muscle-specific E3 ligase MuRF1 impedes pancreatic tumor growth.
Title: Blocking muscle wasting via deletion of the muscle-specific E3 ligase MuRF1 impedes pancreatic tumor growth.
Identification of the MuRF1 Skeletal Muscle Ubiquitylome Through Quantitative Proteomics.
Title: Identification of the MuRF1 Skeletal Muscle Ubiquitylome Through Quantitative Proteomics.
Removal of MuRF1 Increases Muscle Mass in Nemaline Myopathy Models, but Does Not Provide Functional Benefits.
Title: Removal of MuRF1 Increases Muscle Mass in Nemaline Myopathy Models, but Does Not Provide Functional Benefits.
Targeting MuRF1 by small molecules in a HFpEF rat model improves myocardial diastolic function and skeletal muscle contractility.
Title: Targeting MuRF1 by small molecules in a HFpEF rat model improves myocardial diastolic function and skeletal muscle contractility.
MuRF1 deficiency prevents age-related fat weight gain, possibly through accumulation of PDK4 in skeletal muscle mitochondria in older mice.
Title: MuRF1 deficiency prevents age-related fat weight gain, possibly through accumulation of PDK4 in skeletal muscle mitochondria in older mice.
MiR-29ab1 Cluster Resists Muscle Atrophy Through Inhibiting MuRF1.
Title: MiR-29ab1 Cluster Resists Muscle Atrophy Through Inhibiting MuRF1.
Expression of MuRF1 or MuRF2 is essential for the induction of skeletal muscle atrophy and dysfunction in a murine pulmonary hypertension model.
Title: Expression of MuRF1 or MuRF2 is essential for the induction of skeletal muscle atrophy and dysfunction in a murine pulmonary hypertension model.
Regulation of Glucose Metabolism by MuRF1 and Treatment of Myopathy in Diabetic Mice with Small Molecules Targeting MuRF1.
Title: Regulation of Glucose Metabolism by MuRF1 and Treatment of Myopathy in Diabetic Mice with Small Molecules Targeting MuRF1.
Identification and characterization of Fbxl22, a novel skeletal muscle atrophy-promoting E3 ubiquitin ligase.
Title: Identification and characterization of Fbxl22, a novel skeletal muscle atrophy-promoting E3 ubiquitin ligase.

Submit: New GeneRIF Correction See all GeneRIFs (61)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (1)
Targeted (4) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

Trim63 (e-PCR), detects polymorphism
- UniSTS:536427

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables titin binding	ISO Inferred from Sequence Orthology more info
enables ubiquitin protein ligase activity	IBA Inferred from Biological aspect of Ancestor more info
enables ubiquitin protein ligase activity	IDA Inferred from Direct Assay more info	PubMed
enables ubiquitin protein ligase activity	ISO Inferred from Sequence Orthology more info
enables ubiquitin-protein transferase activity	ISO Inferred from Sequence Orthology more info
enables zinc ion binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within cellular response to dexamethasone stimulus	IDA Inferred from Direct Assay more info	PubMed
involved_in innate immune response	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within muscle contraction	ISO Inferred from Sequence Orthology more info	PubMed
acts_upstream_of_or_within myotube cell development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of cardiac muscle hypertrophy	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of glycolytic process	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within proteasome-mediated ubiquitin-dependent protein catabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in protein monoubiquitination	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within regulation of gene expression	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in response to denervation involved in regulation of muscle adaptation	IDA Inferred from Direct Assay more info	PubMed
involved_in response to electrical stimulus involved in regulation of muscle adaptation	ISO Inferred from Sequence Orthology more info
involved_in response to electrical stimulus involved in regulation of muscle adaptation	ISS Inferred from Sequence or Structural Similarity more info

Component	Evidence Code	Pubs
part_of Cul4-RING E3 ubiquitin ligase complex	IDA Inferred from Direct Assay more info	PubMed
located_in M band	IEA Inferred from Electronic Annotation more info
located_in Z disc	IEA Inferred from Electronic Annotation more info
located_in contractile muscle fiber	ISO Inferred from Sequence Orthology more info	PubMed
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in cytosol	TAS Traceable Author Statement more info
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: E3 ubiquitin-protein ligase TRIM63

Names: MURF-1; RING-type E3 ubiquitin transferase TRIM63; muscle ring finger protein 1; muscle-specific RING finger protein 1; ring finger protein 28; tripartite motif-containing protein 63

NP_001034137.2

EC 2.3.2.27

NP_001356174.1

EC 2.3.2.27

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001039048.2 → NP_001034137.2 E3 ubiquitin-protein ligase TRIM63 isoform 1

See identical proteins and their annotated locations for NP_001034137.2

Status: VALIDATED

Description

Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).

Source sequence(s)

AJ278734, AL627253, AL627314, CJ131880, CK128385

Consensus CDS

CCDS38914.1

UniProtKB/TrEMBL

A2A9L4, F8VPZ1

Related

ENSMUSP00000101501.2, ENSMUST00000105875.8

Conserved Domains (3) summary

cd00162
Location:23 → 82

RING; RING-finger (Really Interesting New Gene) domain, a specialized type of Zn-finger of 40 to 60 residues that binds two atoms of zinc; defined by the 'cross-brace' motif C-X2-C-X(9-39)-C-X(1-3)- H-X(2-3)-(N/C/H)-X2-C-X(4-48)C-X2-C; probably involved in ...

pfam00643
Location:120 → 159

zf-B_box; B-box zinc finger

cl23765
Location:166 → 283

iSH2_PI3K_IA_R; Inter-Src homology 2 (iSH2) helical domain of Class IA Phosphoinositide 3-kinase Regulatory subunits
NM_001369245.1 → NP_001356174.1 E3 ubiquitin-protein ligase TRIM63 isoform 2

Status: VALIDATED

Source sequence(s)

AL627253, AL627314

UniProtKB/TrEMBL

A2A9L4, B9EJZ0

Conserved Domains (3) summary

smart00502
Location:166 → 283

BBC; B-Box C-terminal domain

pfam00643
Location:120 → 159

zf-B_box; B-box zinc finger

cd16759
Location:18 → 80

RING-HC_MuRF1; RING finger, HC subclass, found in muscle-specific RING finger protein 1 (MuRF-1) and similar proteins