dapA 4-hydroxy-tetrahydrodipicolinate synthase [Escherichia coli str. K-12 substr. MG1655] - Gene

Summary

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Official Symbol: dapA
Official Full Name: 4-hydroxy-tetrahydrodipicolinate synthase
Primary source: ECOCYC:EG10205
Locus tag: b2478
See related: ASAP:ABE-0008163
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage: Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as: ECK2474
Summary: DapA levels may be influenced by GroES and GroEL. [More information is available at EcoGene: EG10205]. Lys-161 is the active-site lysine residue of DHDPS. [More information is available at EcoCyc: EG10205].
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Genomic context

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Sequence:: NC_000913.3 (2598882..2599760, complement)

NC_000913.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

This study investigated the C-terminal domain of E. coli DHDPS by characterising a C-terminal truncated DHDPS (DHDPS-H225*).
Title: The C-terminal domain of Escherichia coli dihydrodipicolinate synthase (DHDPS) is essential for maintenance of quaternary structure and efficient catalysis.
to probe the mechanism of dihydrodipicolinate synthase (DHDPS), inhibition of DHDPS by the substrate analog, beta-hydroxypyruvate was studied; crystal structure of DHDPS complexed with beta-hydroxypyruvate was solved
Title: Conserved main-chain peptide distortions: a proposed role for Ile203 in catalysis by dihydrodipicolinate synthase.
Results suggest that the homotetrameric structure of dihydrodipicolinate synthase reduces dynamic fluctuations present in the dimeric forms and increases specificity for the first substrate, pyruvate.
Title: Evolution of quaternary structure in a homotetrameric enzyme.
N-terminus verified by Edman degradation on complete protein
Title: '98 Escherichia coli SWISS-2DPAGE database update.
crystal structures of native and (S)-lysine-bound dihydrodipicolinate synthase from E. coli are presented to 1.9 and 2.0 A, respectively
Title: The crystal structures of native and (S)-lysine-bound dihydrodipicolinate synthase from Escherichia coli with improved resolution show new features of biological significance.
evidence that Arg138 of dihydrodipicolinate synthase is responsible for binding the carboxyl of (S)-ASA and is not involved in the mechanism of (S)-lysine inhibition
Title: Role of arginine 138 in the catalysis and regulation of Escherichia coli dihydrodipicolinate synthase.
The dapA promoter is activated by diaminopimelic acid.
Title: Expression from the Escherichia coli dapA promoter is regulated by intracellular levels of diaminopimelic acid.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by EcoCyc

Function	Evidence Code	Pubs
enables 4-hydroxy-tetrahydrodipicolinate synthase activity	IBA Inferred from Biological aspect of Ancestor more info
enables 4-hydroxy-tetrahydrodipicolinate synthase activity	IDA Inferred from Direct Assay more info	PubMed
enables 4-hydroxy-tetrahydrodipicolinate synthase activity	IEA Inferred from Electronic Annotation more info
enables identical protein binding	IDA Inferred from Direct Assay more info	PubMed
enables identical protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables lyase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within diaminopimelate biosynthetic process	IDA Inferred from Direct Assay more info	PubMed
involved_in diaminopimelate biosynthetic process	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within diaminopimelate biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in lysine biosynthetic process	IEA Inferred from Electronic Annotation more info
involved_in lysine biosynthetic process via diaminopimelate	IEA Inferred from Electronic Annotation more info
involved_in small molecule metabolic process	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytosol	HDA more info	PubMed
is_active_in cytosol	IBA Inferred from Biological aspect of Ancestor more info
located_in cytosol	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: 4-hydroxy-tetrahydrodipicolinate synthase

NP_416973.1

EC 4.3.3.7

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_000913.3 Reference assembly

Range

2598882..2599760 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NP_416973.1 4-hydroxy-tetrahydrodipicolinate synthase [Escherichia coli str. K-12 substr. MG1655]

See identical proteins and their annotated locations for NP_416973.1

Status: PROVISIONAL

UniProtKB/TrEMBL

A0A8S0FP06

Conserved Domains (1) summary

PRK03170
Location:1 → 292

PRK03170; dihydrodipicolinate synthase; Provisional