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    fimD type I fimbriae usher protein [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 948844, updated on 3-Dec-2024

    Summary

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    Official Symbol
    fimD
    Official Full Name
    type I fimbriae usher protein
    Primary source
    ECOCYC:EG10311
    Locus tag
    b4317
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK4308; fimD_1; fimD_2
    Summary
    FimD forms pores in the outer membrane. [More information is available at EcoGene: EG10311]. FimD is a member of the Fimbrial Usher Porin (FUP) family . [More information is available at EcoCyc: EG10311].
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    Genomic context

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    Sequence:
    NC_000913.3 (4545096..4547732)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene putative fimbrial protein FimI Neighboring gene type 1 fimbriae periplasmic chaperone Neighboring gene type 1 fimbriae minor subunit FimF Neighboring gene type 1 fimbriae minor subunit FimG

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Allosteric signalling in the outer membrane translocation domain of PapC usher. Farabella I, et al. Elife, 2014 Oct 28. PMID 25271373, Free PMC Article
    2. The usher N terminus is the initial targeting site for chaperone-subunit complexes and participates in subsequent pilus biogenesis events. Ng TW, et al. J Bacteriol, 2004 Aug. PMID 15292133, Free PMC Article
    3. Conformational Dynamics, Intramolecular Domain Conformation Signaling, and Activation of Apo-FimD Revealed by Single-Molecule Fluorescence Resonance Energy Transfer Studies. Liu Y, et al. Biochemistry, 2019 Apr 9. PMID 30888187
    4. Reconstitution of pilus assembly reveals a bacterial outer membrane catalyst. Nishiyama M, et al. Science, 2008 Apr 18. PMID 18369105
    5. The PapC usher forms an oligomeric channel: implications for pilus biogenesis across the outer membrane. Thanassi DG, et al. Proc Natl Acad Sci U S A, 1998 Mar 17. PMID 9501230, Free PMC Article

    See all (48) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Data show that the substrate-free usher FimD (apo-FimD) adopts a contracted conformation that is distinct from its substrate-bound states.
      Title: Conformational Dynamics, Intramolecular Domain Conformation Signaling, and Activation of Apo-FimD Revealed by Single-Molecule Fluorescence Resonance Energy Transfer Studies.
    2. This study describes the gating mechanism of PapC ushers and its importance in maintaining outer-membrane permeability.
      Title: Allosteric signalling in the outer membrane translocation domain of PapC usher.
    3. The results indicate that FimD usher follows the SurA-BamB pathway for its assembly.
      Title: The fimbrial usher FimD follows the SurA-BamB pathway for its assembly in the outer membrane of Escherichia coli.
    4. The catalytic activity of FimD is required for extension of the pilus fiber.
      Title: Function of the usher N-terminus in catalysing pilus assembly.
    5. These data indicate that residues G15, N46 and D47 play an important role following initial binding of FimH to FimD for efficient type 1 fimbriae polymerization by this outer membrane usher.
      Title: Specific residues in the N-terminal domain of FimH stimulate type 1 fimbriae assembly in Escherichia coli following the initial binding of the adhesin to FimD usher.
    6. PapC and FimD of Uropathogenic E coli are ushers bound to a translocating type 1 pilus assembly intermediate; they capture the secretion of a virulence factor across the outer membrane.
      Title: Fiber formation across the bacterial outer membrane by the chaperone/usher pathway.
    7. study of reconstituted type 1 pilus biogenesis from purified pilus proteins; the usher FimD acted as a catalyst to accelerate the ordered assembly of protein subunits independently of cellular energy
      Title: Reconstitution of pilus assembly reveals a bacterial outer membrane catalyst.
    8. N-terminus verified by Edman degradation on mature peptide
      Title: Identification and characterization of the chaperone-subunit complex-binding domain from the type 1 pilus assembly platform FimD.
    9. The lectin domain of FimH recognized by FimD usher is an essential step for type 1 pilus biogenesis.
      Title: Recognition of the N-terminal lectin domain of FimH adhesin by the usher FimD is required for type 1 pilus biogenesis.
    Submit: New GeneRIF Correction

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables fimbrial usher porin activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables fimbrial usher porin activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables fimbrial usher porin activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in pilus assembly IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    acts_upstream_of_or_within pilus assembly IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in pilus assembly IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in transmembrane transport IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    is_active_in cell outer membrane IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in cell outer membrane IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in cell outer membrane IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in membrane IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

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    Preferred Names
    type I fimbriae usher protein

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      4545096..4547732
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_418737.1 type I fimbriae usher protein [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_418737.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A8S7EI66
      Conserved Domains (1) summary
      COG3188
      Location:11 → 878
      FimD; Outer membrane usher protein FimD/PapC [Cell motility, Extracellular structures]
    Nucleotide Protein
    Heading Accession and Version

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