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    yhdJ DNA adenine methyltransferase [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 947695, updated on 3-Dec-2024

    Summary

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    Official Symbol
    yhdJ
    Official Full Name
    DNA adenine methyltransferase
    Primary source
    ECOCYC:EG11498
    Locus tag
    b3262
    See related
    ASAP:ABE-0010706
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK3249
    Summary
    In vitro DNA adenine methyltransferase activity has been demonstrated for YhdJ; overexpression of YhdJ leads to protection of chromosomal DNA from NsiI digestion, but DNA from wild type cells is not protected (Broadbent (2007). [More information is available at EcoGene: EG11498]. Overexpression of YdhJ leads to methylation of genomic DNA at the NsiI recognition sequence (5'-ATGCAT-3'), and partially purified YdhJ is shown to methylate this sequence to produce N6-methyladenine in the 3' adenine position of ATGCAT in vitro . ydhJ is not an essential gene, and overexpression of ydhJ does not alter cell morphology . [More information is available at EcoCyc: EG11498].
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    Genomic context

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    Sequence:
    NC_000913.3 (3411653..3412537)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene tRNA-dihydrouridine synthase B Neighboring gene DNA-binding transcriptional dual regulator Fis Neighboring gene DUF2556 domain-containing protein YhdU Neighboring gene DNA-binding transcriptional repressor EnvR

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. YhdJ, a nonessential CcrM-like DNA methyltransferase of Escherichia coli and Salmonella enterica. Broadbent SE, et al. J Bacteriol, 2007 Jun. PMID 17400740, Free PMC Article
    2. Isolation of the gene encoding the Hin recombinational enhancer binding protein. Johnson RC, et al. Proc Natl Acad Sci U S A, 1988 May. PMID 2835774, Free PMC Article
    3. Dramatic changes in Fis levels upon nutrient upshift in Escherichia coli. Ball CA, et al. J Bacteriol, 1992 Dec. PMID 1459953, Free PMC Article
    4. Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Baba T, et al. Mol Syst Biol, 2006. PMID 16738554, Free PMC Article
    5. The binary protein-protein interaction landscape of Escherichia coli. Rajagopala SV, et al. Nat Biotechnol, 2014 Mar. PMID 24561554, Free PMC Article

    See all (8) citations in PubMed

    GeneRIFs: Gene References Into Functions

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables DNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables N-methyltransferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables methyltransferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables site-specific DNA-methyltransferase (adenine-specific) activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables site-specific DNA-methyltransferase (adenine-specific) activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables site-specific DNA-methyltransferase (adenine-specific) activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in methylation IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    is_active_in cytoplasm IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  

    General protein information

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    Preferred Names
    DNA adenine methyltransferase
    NP_417728.4

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      3411653..3412537
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_417728.4 DNA adenine methyltransferase [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_417728.4

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A1X3JDF4
      Conserved Domains (1) summary
      PRK11524
      Location:7 → 290
      PRK11524; adenine-specific DNA-methyltransferase
    Nucleotide Protein
    Heading Accession and Version

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