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    nhaA Na(+):H(+) antiporter NhaA [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 944758, updated on 3-Dec-2024

    Summary

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    Official Symbol
    nhaA
    Official Full Name
    Na(+):H(+) antiporter NhaA
    Primary source
    ECOCYC:EG10652
    Locus tag
    b0019
    See related
    ASAP:ABE-0000068
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ant; antA; ECK0020
    Summary
    nhaA P2 promoter is RpoS-regulated, but H-NS, Na(+)- and NhaR-independent. [More information is available at EcoGene: EG10652]. NhaA is a sodium ion/proton antiporter that uses the proton electrochemical gradient to expel sodium ions from the cytoplasm and functions primarily in the adaptation to high salinity at alkaline pH. [More information is available at EcoCyc: EG10652].
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    Genomic context

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    Sequence:
    NC_000913.3 (17489..18655)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene protein HokC Neighboring gene regulatory protein MokC Neighboring gene ncRNA Neighboring gene DNA-binding transcriptional activator NhaR Neighboring gene IS1 family protein InsB Neighboring gene IS1 family protein InsA

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Controlled unfolding and refolding of a single sodium-proton antiporter using atomic force microscopy. Kedrov A, et al. J Mol Biol, 2004 Jul 23. PMID 15236973
    2. Functional role of polar amino acid residues in Na+/H+ exchangers. Wiebe CA, et al. Biochem J, 2001 Jul 1. PMID 11415429, Free PMC Article
    3. Screening of environmental DNA libraries for the presence of genes conferring Na(+)(Li(+))/H(+) antiporter activity on Escherichia coli: characterization of the recovered genes and the corresponding gene products. Majerník A, et al. J Bacteriol, 2001 Nov. PMID 11673435, Free PMC Article
    4. Molecular model of the Escherichia coli Na1/H1 antiporter NhaA. Ravna AW, et al. Recept Channels, 2001. PMID 11697236
    5. High level production of functional antibody Fab fragments in an oxidizing bacterial cytoplasm. Venturi M, et al. J Mol Biol, 2002 Jan 4. PMID 11771962

    See all (119) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Site-directed mutagenesis at the Glu78 in Ec-NhaA transporter impacting ion exchange: a biophysical study.
      Title: Site-directed mutagenesis at the Glu78 in Ec-NhaA transporter impacting ion exchange: a biophysical study.
    2. Towards Molecular Understanding of the pH Dependence Characterizing NhaA of Which Structural Fold is Shared by Other Transporters.
      Title: Towards Molecular Understanding of the pH Dependence Characterizing NhaA of Which Structural Fold is Shared by Other Transporters.
    3. An angular motion of a conserved four-helix bundle facilitates alternating access transport in the TtNapA and EcNhaA transporters.
      Title: An angular motion of a conserved four-helix bundle facilitates alternating access transport in the TtNapA and EcNhaA transporters.
    4. Alternative proton-binding site and long-distance coupling in Escherichia coli sodium-proton antiporter NhaA.
      Title: Alternative proton-binding site and long-distance coupling in Escherichia coli sodium-proton antiporter NhaA.
    5. findings indicate that alternative mechanisms sustain EcNhaA activity in the absence of canonical ion-binding residues and that the conserved lysines confer structural stability.
      Title: Replacement of Lys-300 with a glutamine in the NhaA Na(+)/H(+) antiporter of Escherichia coli yields a functional electrogenic transporter.
    6. analysis of NhaA pH-dependent activation by molecular dynamics simulation of atomic details of proton-coupled transport across membrane
      Title: Mechanism of pH-dependent activation of the sodium-proton antiporter NhaA.
    7. Lysine 300 has an essential role in stability but not electrogenic transport of Escherichia coli NhaA
      Title: Lysine 300 is essential for stability but not for electrogenic transport of the Escherichia coli NhaA Na(+)/H(+) antiporter.
    8. Data show that the Li(+) binding, H(+) release and antiporter activity were all affected to the same extent by Na(+), Li(+)/H(+) antiporter (NhaA) mutations in the Li(+) binding site (D163E, D163N, D164N, D164E).
      Title: The Ec-NhaA antiporter switches from antagonistic to synergistic antiport upon a single point mutation.
    9. Data show that the two aspartic acid residues of Na+/H+ antiporter NhaA, D163 and D164, act as Na+ trap and energetic barrier for the transport of Na+.
      Title: Simulating the function of sodium/proton antiporters.
    10. NhaA antiporter functions using 10 helices, and an additional 2 contribute to assembly/stability.
      Title: NhaA antiporter functions using 10 helices, and an additional 2 contribute to assembly/stability.
    Submit: New GeneRIF Correction See all GeneRIFs (27)

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables antiporter activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables cardiolipin binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables sodium ion transmembrane transporter activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables sodium:proton antiporter activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables sodium:proton antiporter activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in regulation of intracellular pH TAS
    Traceable Author Statement
    more info
    		 PubMed 
    involved_in regulation of pH IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in response to alkaline pH IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in response to alkaline pH IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in response to salt stress IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in sodium ion transmembrane transport IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in sodium ion transport IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in transmembrane transport IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    located_in membrane IEA
    Inferred from Electronic Annotation
    more info
    		  
    is_active_in plasma membrane IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in plasma membrane IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in plasma membrane IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in plasma membrane ISM
    Inferred from Sequence Model
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    Na(+):H(+) antiporter NhaA

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      17489..18655
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_414560.1 Na(+):H(+) antiporter NhaA [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_414560.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A8S7CVL3, A0A8S7I7R4
      Conserved Domains (1) summary
      PRK09561
      Location:1 → 388
      nhaA; sodium/proton antiporter NhaA
    Nucleotide Protein
    Heading Accession and Version

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