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    metJ DNA-binding transcriptional repressor MetJ [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 948435, updated on 17-Dec-2024

    Summary

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    Official Symbol
    metJ
    Official Full Name
    DNA-binding transcriptional repressor MetJ
    Primary source
    ECOCYC:EG10588
    Locus tag
    b3938
    See related
    ASAP:ABE-0012874
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK3930
    Summary
    Upregulated by GSNO in defined medium (aerobic). [More information is available at EcoGene: EG10588].
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    Genomic context

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    Sequence:
    NC_000913.3 (4128078..4128395, complement)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene 50S ribosomal subunit protein L31 Neighboring gene putative lipid-binding hydrolase YiiX Neighboring gene O-succinylhomoserine(thiol)-lyase/O-succinylhomoserine lyase Neighboring gene fused aspartate kinase/homoserine dehydrogenase 2

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. The Escherichia coli K-12 metJ193 allele contains a point mutation which alters the hydrophobic pocket responsible for in vitro binding of S-adenosylmethionine: effects on cell growth and induction of met regulon expression. Collier CD, et al. J Bacteriol, 1990 Jul. PMID 2141834, Free PMC Article
    2. Conformational model for binding site recognition by the E.coli MetJ transcription factor. Liu R, et al. Bioinformatics, 2001 Jul. PMID 11448880
    3. Mechanism of L-methionine overproduction by Escherichia coli: the replacement of Ser-54 by Asn in the MetJ protein causes the derepression of L-methionine biosynthetic enzymes. Nakamori S, et al. Appl Microbiol Biotechnol, 1999 Aug. PMID 10499257
    4. Kinetic analysis of operator binding by the E. coli methionine repressor highlights the role(s) of electrostatic interactions. Lawrenson ID, et al. FEBS Lett, 2004 Apr 23. PMID 15094055
    5. Cloning of the methionine regulatory gene, metJ, of Escherichia coli K12 and identification of its product. Smith AA, et al. J Biol Chem, 1984 Nov 25. PMID 6389545

    See all (46) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. We found that potentiating mutations in metJ increase expression of metA, which encodes the first step of methionine biosynthesis. This increase in expression is required for the previously identified actualizing mutations in metA to generate cooperation.
      Title: Identification of the potentiating mutations and synergistic epistasis that enabled the evolution of inter-species cooperation.
    2. N-terminus verified by Edman degradation on mature peptide
      Title: Structure and autoregulation of the metJ regulatory gene in Escherichia coli.
    3. Transcript analysis reveals an extended regulon and the importance of protein-protein co-operativity for the Escherichia coli methionine repressor
      Title: Transcript analysis reveals an extended regulon and the importance of protein-protein co-operativity for the Escherichia coli methionine repressor.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables DNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables DNA-binding transcription factor activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in methionine biosynthetic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in methionine metabolic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in negative regulation of DNA-templated transcription IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in regulation of DNA-templated transcription IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    DNA-binding transcriptional repressor MetJ

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      4128078..4128395 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_418373.1 DNA-binding transcriptional repressor MetJ [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_418373.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A1X3HZT1, A0A1X3ITW5, A0A829LEI9, A0AAJ3NUW2, A0AAN3M6I8
      Conserved Domains (1) summary
      PRK05264
      Location:1 → 105
      PRK05264; met regulon transcriptional regulator MetJ
    Nucleotide Protein
    Heading Accession and Version

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