gadA glutamate decarboxylase A [Escherichia coli str. K-12 substr. MG1655] - Gene

Summary

Official Symbol: gadA
Official Full Name: glutamate decarboxylase A
Primary source: ECOCYC:EG50009
Locus tag: b3517
See related: ASAP:ABE-0011490
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage: Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as: ECK3502; gadS
Summary: RpoS regulon. [More information is available at EcoGene: EG50009]. GadA, a glutamate decarboxylase enzyme, is part of the glutamate-dependent acid resistance system 2 (AR2) which confers resistance to extreme acid conditions. [More information is available at EcoCyc: EG50009].
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NC_000913.3 Genomic Context describing neighboring genes

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Data show that Corynebacterium glutamicum expressing E. coli glutamate decarboxylase (GAD) mutant under the strong PH36 promoter could produce GABA 17-fold higher than that obtained by C. glutamicum expressing wild-type E. coli GAD.
Title: Enhanced production of gamma-aminobutyrate (GABA) in recombinant Corynebacterium glutamicum by expressing glutamate decarboxylase active in expanded pH range.
activation of gadA transcription requires a GAD box and binding of an RcsB/GadE heterodimer.
Title: Acid stress response in Escherichia coli: mechanism of regulation of gadA transcription by RcsB and GadE.
N-terminus verified by Edman degradation on complete protein
Title: Acid- and base-induced proteins during aerobic and anaerobic growth of Escherichia coli revealed by two-dimensional gel electrophoresis.
H-NS directly inhibits gadA and gadX transcription and, by controlling the intracellular level of the activator GadX, indirectly affects the expression of the whole gad system
Title: Antagonistic role of H-NS and GadX in the regulation of the glutamate decarboxylase-dependent acid resistance system in Escherichia coli.

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

Function	Evidence Code	Pubs
enables carboxy-lyase activity	IEA Inferred from Electronic Annotation more info
enables glutamate decarboxylase activity	IBA Inferred from Biological aspect of Ancestor more info
enables glutamate decarboxylase activity	IDA Inferred from Direct Assay more info	PubMed
enables glutamate decarboxylase activity	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables pyridoxal phosphate binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in glutamate catabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in glutamate metabolic process	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within intracellular pH elevation	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in cytosol	HDA more info	PubMed
is_active_in cytosol	IBA Inferred from Biological aspect of Ancestor more info
located_in membrane	HDA more info	PubMed

NP_417974.1

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