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    uvrD DNA helicase II [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 948347, updated on 17-Dec-2024

    Summary

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    Official Symbol
    uvrD
    Official Full Name
    DNA helicase II
    Primary source
    ECOCYC:EG11064
    Locus tag
    b3813
    See related
    ASAP:ABE-0012455
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    dar-2; dda; ECK3808; mutU; pdeB; rad; recL; srjC; uvr502; uvrE
    Summary
    The completion of nucleotide excision repair depends upon the release of the oligonucleotide fragment along with the UvrB and UvrC proteins which are then able to be recycled to undertake another excision repair event. [More information is available at EcoCyc: EG11064].
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    Genomic context

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    Sequence:
    NC_000913.3 (3997983..4000145)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene DUF484 domain-containing protein YigA Neighboring gene site-specific tyrosine recombinase Neighboring gene 5-amino-6-(5-phospho-D-ribitylamino)uracil phosphatase Neighboring gene DUF2233 domain-containing protein YigE Neighboring gene protein YsgD

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Mechanism of ATP-dependent translocation of E.coli UvrD monomers along single-stranded DNA. Fischer CJ, et al. J Mol Biol, 2004 Dec 10. PMID 15561144
    2. UvrD303, a hyperhelicase mutant that antagonizes RecA-dependent SOS expression by a mechanism that depends on its C terminus. Centore RC, et al. J Bacteriol, 2009 Mar. PMID 19074381, Free PMC Article
    3. Impediment of E. coli UvrD by DNA-destabilizing force reveals a strained-inchworm mechanism of DNA unwinding. Sun B, et al. EMBO J, 2008 Dec 17. PMID 19008855, Free PMC Article
    4. Transcriptional control of the uvrD gene of Escherichia coli. Arthur HM, et al. Gene, 1983 Nov. PMID 6319240
    5. Nucleotide sequence of the regulatory region of the uvrD gene of Escherichia coli. Finch P, et al. Gene, 1983 Nov. PMID 6319241

    See all (123) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Recipient UvrD helicase is involved in single- to double-stranded DNA conversion during conjugative plasmid transfer.
      Title: Recipient UvrD helicase is involved in single- to double-stranded DNA conversion during conjugative plasmid transfer.
    2. MutL Escherichia coli
      Title: UvrD helicase activation by MutL involves rotation of its 2B subdomain.
    3. Observations are consistent with an exonuclease-independent MMR strand excision mechanism that relies on EcMutL-EcUvrD helicase-driven displacement of ssDNA segments between adjacent EcMutH-GATC incisions.
      Title: MutL sliding clamps coordinate exonuclease-independent Escherichia coli mismatch repair.
    4. Rep and UvrD helicases operate on DNA at the replication fork whereas DinG helicase acts via a different mechanism
      Title: Direct removal of RNA polymerase barriers to replication by accessory replicative helicases.
    5. The lowest free-energy path shows that UvrD opens the interface between two domains, allowing the bound ssDNA to escape. The simulation results predict a key metastable 'tilted' state during ssDNA strand switching.
      Title: Free-energy simulations reveal molecular mechanism for functional switch of a DNA helicase.
    6. Large domain movements upon UvrD dimerization and helicase activation has been reported.
      Title: Large domain movements upon UvrD dimerization and helicase activation.
    7. Results show that UvrD monomer translocation rate depends on the single-stranded DNA base composition, even in the absence of any predicted basepairing within the nucleic acid. Translocation is faster on single-stranded DNA-containing pyrimidines and slower on ssDNA-containing purines
      Title: Modulation of Escherichia coli UvrD Single-Stranded DNA Translocation by DNA Base Composition.
    8. these data reveal that UvrD exhibits two distinct types of unwinding activity regulated by its stoichiometry. Furthermore, two UvrD conformational states, termed "closed" and "open," correlate with movement toward or away from the DNA fork.
      Title: Protein structure. Direct observation of structure-function relationship in a nucleic acid-processing enzyme.
    9. The unwinding processivity is increased in the UvrD303 mutant.
      Title: The UvrD303 hyper-helicase exhibits increased processivity.
    10. it is hypothesized that recA4162 suppresses SOS expression best when the ssDNA occurs at a gap and that uvrD303 is able to decrease SOS expression when the ssDNA is either at a gap or when it is generated at a DSB but does so better at a gap
      Title: Specificity in suppression of SOS expression by recA4162 and uvrD303.
    Submit: New GeneRIF Correction See all GeneRIFs (33)

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables 3'-5' DNA helicase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables 3'-5' DNA helicase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables ATP binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables ATP hydrolysis activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables ATP-dependent H2AZ histone chaperone activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables ATP-dependent H3-H4 histone complex chaperone activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables DNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables DNA clamp loader activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables DNA helicase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables DNA helicase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables DNA translocase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables chromatin extrusion motor activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables cohesin loader activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables double-stranded DNA helicase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables forked DNA-dependent helicase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables four-way junction helicase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables helicase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables hydrolase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables isomerase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables protein homodimerization activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables single-stranded 3'-5' DNA helicase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables single-stranded DNA helicase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Process Evidence Code Pubs
    acts_upstream_of_or_within DNA duplex unwinding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in DNA repair IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in DNA replication IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in DNA unwinding involved in DNA replication IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within SOS response EXP
    Inferred from Experiment
    more info
    		 PubMed 
    involved_in SOS response IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in chromatin looping IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in chromatin remodeling IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within mismatch repair EXP
    Inferred from Experiment
    more info
    		 PubMed 
    acts_upstream_of_or_within mismatch repair IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in mismatch repair involved in maintenance of fidelity involved in DNA-dependent DNA replication IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    acts_upstream_of_or_within nucleotide-excision repair IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in nucleotide-excision repair, DNA duplex unwinding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in recombinational repair IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    acts_upstream_of_or_within replication fork processing EXP
    Inferred from Experiment
    more info
    		 PubMed 
    acts_upstream_of_or_within replication fork processing IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within response to radiation IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within rolling circle DNA replication IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    part_of DNA helicase complex IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    part_of DNA helicase complex IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  
    is_active_in cytosol IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    part_of single-stranded DNA-dependent ATP-dependent DNA helicase complex IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    DNA helicase II

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      3997983..4000145
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_418258.1 DNA helicase II [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_418258.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A1X3J9Z5
      Conserved Domains (1) summary
      PRK11773
      Location:1 → 720
      uvrD; DNA-dependent helicase II; Provisional
    Nucleotide Protein
    Heading Accession and Version

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