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    dnaT primosomal protein DnaT [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 948813, updated on 3-Dec-2024

    Summary

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    Official Symbol
    dnaT
    Official Full Name
    primosomal protein DnaT
    Primary source
    ECOCYC:EG10244
    Locus tag
    b4362
    See related
    ASAP:ABE-0014306
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK4352
    Summary
    DnaT is required for chromosomal DNA replication and for induction of replication in the absence of protein synthesis during the SOS response . [More information is available at EcoCyc: EG10244].
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    Genomic context

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    Sequence:
    NC_000913.3 (4600978..4601517, complement)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene DUF2501 domain-containing protein YjjA Neighboring gene DNA replication protein DnaC Neighboring gene putative succinate exporter YjjB Neighboring gene putative succinate exporter YjjP Neighboring gene DNA-binding transcriptional repressor YjjQ Neighboring gene DNA-binding transcriptional regulator BglJ

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. A multienzyme system for priming the replication of phiX174 viral DNA. McMacken R, et al. J Biol Chem, 1978 May 10. PMID 346590
    2. Effects of Mg2+ and denaturants on the unfolding pattern of DNA-T--a replication protein of E. coli. Antony T, et al. Int J Biol Macromol, 1996 Aug. PMID 8842771
    3. dnaT, dominant conditional-lethal mutation affecting DNA replication in Escherichia coli. Lark CA, et al. J Bacteriol, 1978 Dec. PMID 363684, Free PMC Article
    4. Energetics of the Escherichia coli DnaT protein trimerization reaction. Szymanski MR, et al. Biochemistry, 2013 Mar 19. PMID 23418702, Free PMC Article
    5. The Escherichia coli primosomal DnaT protein exists in solution as a monomer-trimer equilibrium system. Szymanski MR, et al. Biochemistry, 2013 Mar 19. PMID 23418648, Free PMC Article

    See all (39) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. A structural model of the PriB-DnaT complex in Escherichia coli replication restart.
      Title: A structural model of the PriB-DnaT complex in Escherichia coli replication restart.
    2. These results constitute a pioneering study of the DnaT-PriC interaction and present a putative link between the two independent replication restart pathways, namely, PriA- and PriC-dependent primosome assemblies. Further research can directly focus on determining how DnaT binds to the PriC-SSB-DNA tricomplex and regulates the PriC-dependent replication restart.
      Title: DnaT is a PriC-binding protein.
    3. Structural insight into the DNA-binding mode of the primosomal proteins PriA, PriB, and DnaT.
      Title: Structural insight into the DNA-binding mode of the primosomal proteins PriA, PriB, and DnaT.
    4. provide a novel structural basis for understanding the mechanism of DnaT in exposure of ssDNA and reloading of the replicative helicase at the stalled replication fork
      Title: Structure and mechanism of the primosome protein DnaT-functional structures for homotrimerization, dissociation of ssDNA from the PriB·ssDNA complex, and formation of the DnaT·ssDNA complex.
    5. DnaT can cooperatively bind to ssDNA to form rod-like nucleoprotein filaments, which indicates that the DnaT-ssDNA complex might function as a scaffold during primosome assembly.
      Title: Crystal structure of DnaT84-153-dT10 ssDNA complex reveals a novel single-stranded DNA binding mode.
    6. N-terminus verified by Edman degradation on mature peptide
      Title: Cloning of the Escherichia coli gene for primosomal protein i: the relationship to dnaT, essential for chromosomal DNA replication.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables identical protein binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables identical protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables magnesium ion binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables single-stranded DNA binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in DNA replication IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in DNA replication, synthesis of primer IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in DNA replication, synthesis of primer NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    involved_in DNA unwinding involved in DNA replication NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    acts_upstream_of_or_within DNA-templated DNA replication IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within protein homotrimerization IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    acts_upstream_of_or_within replication fork processing IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in replication fork processing NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    Component Evidence Code Pubs
    part_of DnaB-DnaC-DnaT-PriA-PriB complex IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    part_of DnaB-DnaC-DnaT-PriA-PriB complex NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    part_of DnaB-DnaC-DnaT-PriA-PriC complex NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    part_of primosome complex IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    part_of primosome complex IEA
    Inferred from Electronic Annotation
    more info
    		  
    part_of primosome complex NAS
    Non-traceable Author Statement
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    primosomal protein DnaT

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      4600978..4601517 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_418782.1 primosomal protein DnaT [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_418782.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A2H4TQZ1, A0A2X1N309, A0A5B9ALJ7, Q7WVY3
      Conserved Domains (1) summary
      PRK02854
      Location:1 → 179
      PRK02854; primosomal protein DnaT
    Nucleotide Protein
    Heading Accession and Version

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