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    nuoK NADH:quinone oxidoreductase subunit K [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 947580, updated on 3-Dec-2024

    Summary

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    Official Symbol
    nuoK
    Official Full Name
    NADH:quinone oxidoreductase subunit K
    Primary source
    ECOCYC:EG12091
    Locus tag
    b2279
    See related
    ASAP:ABE-0007534
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK2273
    Summary
    NuoK is part of the inner membrane component of NADH dehydrogenase I . [More information is available at EcoCyc: EG12091].
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    Genomic context

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    Sequence:
    NC_000913.3 (2395043..2395345, complement)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene NADH:quinone oxidoreductase subunit N Neighboring gene NADH:quinone oxidoreductase subunit M Neighboring gene NADH:quinone oxidoreductase subunit L Neighboring gene NADH:quinone oxidoreductase subunit J Neighboring gene NADH:quinone oxidoreductase subunit I Neighboring gene NADH:quinone oxidoreductase subunit H Neighboring gene NADH:quinone oxidoreductase subunit G

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Conserved negative charges in the transmembrane segments of subunit K of the NADH:ubiquinone oxidoreductase determine its dependence on YidC for membrane insertion. Price CE, et al. J Biol Chem, 2010 Feb 5. PMID 19959836, Free PMC Article
    2. The MELAS mutations 3946 and 3949 perturb the critical structure in a conserved loop of the ND1 subunit of mitochondrial complex I. Kervinen M, et al. Hum Mol Genet, 2006 Sep 1. PMID 16849371
    3. Electrostatics, hydration, and proton transfer dynamics in the membrane domain of respiratory complex I. Kaila VR, et al. Proc Natl Acad Sci U S A, 2014 May 13. PMID 24778264, Free PMC Article
    4. Mutagenesis of subunit N of the Escherichia coli complex I. Identification of the initiation codon and the sensitivity of mutants to decylubiquinone. Amarneh B, et al. Biochemistry, 2003 May 6. PMID 12718520
    5. Inhibition by capsaicin of NADH-quinone oxidoreductases is correlated with the presence of energy-coupling site 1 in various organisms. Yagi T. Arch Biochem Biophys, 1990 Sep. PMID 2118334

    See all (77) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. We observe spontaneous hydration of a putative proton entry channel at the NuoN/K interface, which is sensitive to the protonation state of buried glutamic acid residues.
      Title: Electrostatics, hydration, and proton transfer dynamics in the membrane domain of respiratory complex I.
    2. Results suggest a model in which YidC serves as a membrane chaperone for the insertion of the less hydrophobic, negatively charged transmembrane segments of NuoK.
      Title: Conserved negative charges in the transmembrane segments of subunit K of the NADH:ubiquinone oxidoreductase determine its dependence on YidC for membrane insertion.
    3. Membrane-embedded conserved acidic residues glutamate-36 and glutamate-72 are important for the coupling mechanism of mitochondrial ND4L subunit NuoK of NADH-quinone oxidoreductase (NDH-1).
      Title: Characterization of the membrane domain subunit NuoK (ND4L) of the NADH-quinone oxidoreductase from Escherichia coli.
    4. The results provide new information on the function of the region affected by the MELAS mutations 3946 and 3949 that is not obtainable from patient samples or current eukaryote models.
      Title: The MELAS mutations 3946 and 3949 perturb the critical structure in a conserved loop of the ND1 subunit of mitochondrial complex I.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    contributes_to NADH dehydrogenase (ubiquinone) activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables NADH dehydrogenase (ubiquinone) activity IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    contributes_to NADH dehydrogenase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables NADH:ubiquinone reductase (non-electrogenic) activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables oxidoreductase activity, acting on NAD(P)H IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables quinone binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in ATP synthesis coupled electron transport IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in proton transmembrane transport IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in respiratory electron transport chain IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Component Evidence Code Pubs
    part_of NADH dehydrogenase complex IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    part_of NADH dehydrogenase complex IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in membrane IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in plasma membrane IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in plasma membrane IEA
    Inferred from Electronic Annotation
    more info
    		  
    part_of respiratory chain complex I IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    part_of respiratory chain complex I IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    part_of respiratory chain complex I NAS
    Non-traceable Author Statement
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    NADH:quinone oxidoreductase subunit K
    NP_416782.1

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      2395043..2395345 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_416782.1 NADH:quinone oxidoreductase subunit K [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_416782.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A1V2GK77
      Conserved Domains (1) summary
      PRK05715
      Location:1 → 100
      PRK05715; NADH-quinone oxidoreductase subunit NuoK
    Nucleotide Protein
    Heading Accession and Version

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