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    cusB copper/silver export system membrane fusion protein [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 945189, updated on 3-Dec-2024

    Summary

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    Official Symbol
    cusB
    Official Full Name
    copper/silver export system membrane fusion protein
    Primary source
    ECOCYC:G6322
    Locus tag
    b0574
    See related
    ASAP:ABE-0001969
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK0566; ylcD
    Summary
    cusB is in the cusCFBA Cu/Ag efflux operon regulated by Cu and Ag. [More information is available at EcoGene: EG14235]. CusB is a membrane fusion protein involved in the detoxification of copper and silver ions in Escherichia coli as part of the CusCFBA copper/silver efflux system. [More information is available at EcoCyc: G6322].
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    Genomic context

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    Sequence:
    NC_000913.3 (597479..598702)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene copper/silver export system outer membrane channel Neighboring gene copper/silver export system periplasmic binding protein Neighboring gene copper/silver export system RND permease Neighboring gene phenylalanine:H(+) symporter PheP

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Crystallization and preliminary X-ray crystallographic analysis of Escherichia coli CusB. Xu Y, et al. Acta Crystallogr Sect F Struct Biol Cryst Commun, 2009 Jul 1. PMID 19574656, Free PMC Article
    2. EPR Spectroscopy Targets Structural Changes in the E. coli Membrane Fusion CusB upon Cu(I) Binding. Meir A, et al. Biophys J, 2017 Jun 20. PMID 28636907, Free PMC Article
    3. N-terminal region of CusB is sufficient for metal binding and metal transfer with the metallochaperone CusF. Mealman TD, et al. Biochemistry, 2012 Aug 28. PMID 22812620, Free PMC Article
    4. Interactions between CusF and CusB identified by NMR spectroscopy and chemical cross-linking coupled to mass spectrometry. Mealman TD, et al. Biochemistry, 2011 Apr 5. PMID 21323389, Free PMC Article
    5. Silver-resistant mutants of Escherichia coli display active efflux of Ag+ and are deficient in porins. Li XZ, et al. J Bacteriol, 1997 Oct. PMID 9324262, Free PMC Article

    See all (39) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. findings reveal CusB is a dimer in solution and the orientation of one molecule with respect to the other molecule changes upon Cu(I) coordination, resulting in a more compact CusB structure; these structural and topological changes upon Cu(I) binding probably play a role of a switch for opening the channel and transferring metal ions from CusB to CusC and out of the cell
      Title: EPR Spectroscopy Targets Structural Changes in the E. coli Membrane Fusion CusB upon Cu(I) Binding.
    2. N-terminal region of CusB is sufficient for metal binding and metal transfer with the metallochaperone CusF.
      Title: N-terminal region of CusB is sufficient for metal binding and metal transfer with the metallochaperone CusF.
    3. Data reveal that N-terminal region of CusB interacts with metal-binding face of CusF; these proteins transiently interact in a metal-dependent fashion.
      Title: Interactions between CusF and CusB identified by NMR spectroscopy and chemical cross-linking coupled to mass spectrometry.
    4. Alpha-helical domain of CusB is folded into a three-helix bundle that interacts with CusC. N- and C-termini of CusB form the first beta-strand domain that interact with CusA. CusB consists of multiple binding sites for Cu(+) and Ag(+) ions.
      Title: Crystal structure of the membrane fusion protein CusB from Escherichia coli.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables copper ion binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables copper ion transmembrane transporter activity IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables transition metal ion binding IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables transition metal ion binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables transmembrane transporter activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in copper ion export IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    acts_upstream_of_or_within copper ion export IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    involved_in copper ion export NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    involved_in copper ion transmembrane transport NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    involved_in copper ion transport IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within detoxification of copper ion IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    acts_upstream_of_or_within detoxification of copper ion IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within intracellular copper ion homeostasis IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    acts_upstream_of_or_within intracellular copper ion homeostasis IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in plasma membrane copper ion transport IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    acts_upstream_of_or_within plasma membrane copper ion transport IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    acts_upstream_of_or_within response to copper ion IEP
    Inferred from Expression Pattern
    more info
    		 PubMed 
    acts_upstream_of_or_within response to copper ion IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within response to silver ion IEP
    Inferred from Expression Pattern
    more info
    		 PubMed 
    acts_upstream_of_or_within response to silver ion IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in response to toxic substance IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in response to toxic substance NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    involved_in silver ion transmembrane transport NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    involved_in transmembrane transport IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    is_active_in cell envelope IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in membrane IEA
    Inferred from Electronic Annotation
    more info
    		  
    is_active_in outer membrane-bounded periplasmic space IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in outer membrane-bounded periplasmic space IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    copper/silver export system membrane fusion protein

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      597479..598702
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_415106.1 copper/silver export system membrane fusion protein [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_415106.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A8S7EK49
      Conserved Domains (1) summary
      PRK09783
      Location:1 → 407
      PRK09783; copper/silver efflux system membrane fusion protein CusB; Provisional
    Nucleotide Protein
    Heading Accession and Version

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