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    folD bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 945221, updated on 17-Dec-2024

    Summary

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    Official Symbol
    folD
    Official Full Name
    bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase
    Primary source
    ECOCYC:EG10328
    Locus tag
    b0529
    See related
    ASAP:ABE-0001818
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ads; ECK0522
    Summary
    Early purification studies indicated that the bifunctional protein had a structure of five dissimilar subunits. [More information is available at EcoCyc: EG10328].
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    Genomic context

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    Sequence:
    NC_000913.3 (556875..557741, complement)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene PF04307 family inner membrane protein YbcI Neighboring gene putative RNA-binding protein YbcJ Neighboring gene putative fimbrial protein SfmA Neighboring gene putative fimbrial chaperone SfmC

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. A complex of N5,N10-methylenetetrahydrofolate dehydrogenase and N5,N10-methenyltetrahydrofolate cyclohydrolase in Escherichia coli. Purification, subunit structure, and allosteric inhibition by N10-formyltetrahydrofolate. Dev IK, et al. J Biol Chem, 1978 Jun 25. PMID 350870
    2. Physical map location of the Escherichia coli gene encoding the bifunctional enzyme 5,10-methylene-tetrahydrofolate dehydrogenase/5,10-methenyl-tetrahydrofolate cyclohydrolase. Dimri GP, et al. J Bacteriol, 1991 Sep. PMID 1885508, Free PMC Article
    3. Purification and properties of 5,10-methylenetetrahydrofolate dehydrogenase from Escherichia coli. Donaldson KO, et al. J Biol Chem, 1965 Nov. PMID 4378935
    4. Purification, crystallization, and preliminary x-ray studies of a bifunctional 5,10-methenyl/methylene-tetrahydrofolate cyclohydrolase/dehydrogenase from Escherichia coli. Cheung E, et al. Proteins, 1997 Feb. PMID 9061797
    5. The crystal structure of a bacterial, bifunctional 5,10 methylene-tetrahydrofolate dehydrogenase/cyclohydrolase. Shen BW, et al. Protein Sci, 1999 Jun. PMID 10386884, Free PMC Article

    See all (19) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. characterization of wild-type and mutant (R191E, G122D, D121A, Q98K, C58Y, K54S, and Y50S) FolD together with three-dimensional modeling has allowed us to develop a better understanding of the mechanism for substrate binding and catalysis by E. coli FolD.
      Title: Impact of Mutating the Key Residues of a Bifunctional 5,10-Methylenetetrahydrofolate Dehydrogenase-Cyclohydrolase from Escherichia coli on Its Activities.
    2. N-terminus verified by Edman degradation on mature peptide
      Title: Purification, characterization, cloning, and amino acid sequence of the bifunctional enzyme 5,10-methylenetetrahydrofolate dehydrogenase/5,10-methenyltetrahydrofolate cyclohydrolase from Escherichia coli.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables hydrolase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables methenyltetrahydrofolate cyclohydrolase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables methenyltetrahydrofolate cyclohydrolase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables methenyltetrahydrofolate cyclohydrolase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables methylenetetrahydrofolate dehydrogenase (NADP+) activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables methylenetetrahydrofolate dehydrogenase (NADP+) activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables methylenetetrahydrofolate dehydrogenase (NADP+) activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables oxidoreductase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein homodimerization activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in L-histidine biosynthetic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in methionine biosynthetic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within methionine biosynthetic process IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in one-carbon metabolic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in purine nucleotide biosynthetic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in tetrahydrofolate interconversion IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in tetrahydrofolate interconversion IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    is_active_in cytosol IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase
    NP_415062.1

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      556875..557741 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_415062.1 bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_415062.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      W1X0K2
      Conserved Domains (1) summary
      PRK10792
      Location:1 → 285
      PRK10792; bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase FolD
    Nucleotide Protein
    Heading Accession and Version

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