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    entB enterobactin synthase component B [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 946178, updated on 3-Dec-2024

    Summary

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    Official Symbol
    entB
    Official Full Name
    enterobactin synthase component B
    Primary source
    ECOCYC:EG10260
    Locus tag
    b0595
    See related
    ASAP:ABE-0002052
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK0588; entG
    Summary
    Recent gel filtration data suggest that EntB may be a trimer not a pentamer. [More information is available at EcoCyc: EG10260].
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    Genomic context

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    Sequence:
    NC_000913.3 (627694..628551)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene isochorismate synthase EntC Neighboring gene 2,3-dihydroxybenzoate-AMP ligase Neighboring gene 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase Neighboring gene proofreading thioesterase in enterobactin biosynthesis Neighboring gene pyruvate transporter CstA

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Structural and functional investigation of the intermolecular interaction between NRPS adenylation and carrier protein domains. Sundlov JA, et al. Chem Biol, 2012 Feb 24. PMID 22365602, Free PMC Article
    2. Subcloning of the enterobactin biosynthetic gene entB: expression, purification, characterization, and substrate specificity of isochorismatase. Rusnak F, et al. Biochemistry, 1990 Feb 13. PMID 2139796
    3. EntG activity of Escherichia coli enterobactin synthetase. Staab JF, et al. J Bacteriol, 1990 Nov. PMID 2172214, Free PMC Article
    4. Ligand-induced conformational rearrangements promote interaction between the Escherichia coli enterobactin biosynthetic proteins EntE and EntB. Khalil S, et al. J Mol Biol, 2009 Oct 30. PMID 19699210
    5. Structure of the EntB multidomain nonribosomal peptide synthetase and functional analysis of its interaction with the EntE adenylation domain. Drake EJ, et al. Chem Biol, 2006 Apr. PMID 16632253

    See all (27) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. The aryl carrier protein (ArCP) domain of EntB interacts specifically with EntH to help with the proofreading role for EntH during biosynthesis of enterobactin.
      Title: The hotdog thioesterase EntH (YbdB) plays a role in vivo in optimal enterobactin biosynthesis by interacting with the ArCP domain of EntB.
    2. crystal structure of the EntB protein that contains an N-terminal isochorismate lyase domain that functions in the synthesis of 2,3-dihydroxybenzoate and a C-terminal carrier protein domain
      Title: Structure of the EntB multidomain nonribosomal peptide synthetase and functional analysis of its interaction with the EntE adenylation domain.
    Submit: New GeneRIF Correction

    Pathways from PubChem

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    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables 2,3-dihydroxybenzoate-serine ligase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables 2,3-dihydroxybenzoate-serine ligase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables hydrolase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables identical protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables isochorismatase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables isochorismatase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables ligase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables magnesium ion binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables phosphopantetheine binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables transferase activity, transferring alkyl or aryl (other than methyl) groups IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in enterobactin biosynthetic process IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in enterobactin biosynthetic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in plasma membrane IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    enterobactin synthase component B
    NP_415127.1

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      627694..628551
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_415127.1 enterobactin synthase component B [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_415127.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A8S7RNN6
      Conserved Domains (2) summary
      COG1535
      Location:1 → 215
      EntB; Isochorismate hydrolase [Secondary metabolites biosynthesis, transport and catabolism]
      COG3433
      Location:216 → 285
      DhbB2; Aryl carrier domain [Secondary metabolites biosynthesis, transport and catabolism]
    Nucleotide Protein
    Heading Accession and Version

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