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    lpxD UDP-3-O-(3-hydroxymyristoyl)glucosamine N-acyltransferase [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 944882, updated on 3-Dec-2024

    Summary

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    Official Symbol
    lpxD
    Official Full Name
    UDP-3-O-(3-hydroxymyristoyl)glucosamine N-acyltransferase
    Primary source
    ECOCYC:EG10316
    Locus tag
    b0179
    See related
    ASAP:ABE-0000610
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK0178; firA; omsA
    Summary
    LpxD contains 25 hexapeptide repeats. [More information is available at EcoGene: EG10316]. UDP-3-O-(3-hydroxymyristoyl)glucosamine N-acetyltransferase (LpxD) codes for the third step in lipid A biosynthesis. [More information is available at EcoCyc: EG10316].
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    Genomic context

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    Sequence:
    NC_000913.3 (200971..201996)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene outer membrane protein assembly factor BamA Neighboring gene periplasmic chaperone Skp Neighboring gene 3-hydroxy-acyl-[acyl-carrier-protein] dehydratase Neighboring gene acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase Neighboring gene lipid A disaccharide synthase

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. The firA gene, a locus involved in the expression of rifampicin resistance in Escherichia coli. I. Characterisation of lambdafirA transducing phages constructed in vitro. Lathe R, et al. Mol Gen Genet, 1977 Jul 7. PMID 331078
    2. Mutants carrying conditionally lethal mutations in outer membrane genes omsA and firA (ssc) are phenotypically similar, and omsA is allelic to firA. Vuorio R, et al. J Bacteriol, 1992 Nov. PMID 1429432, Free PMC Article
    3. Crystal structure and acyl chain selectivity of Escherichia coli LpxD, the N-acyltransferase of lipid A biosynthesis. Bartling CM, et al. Biochemistry, 2009 Sep 15. PMID 19655786, Free PMC Article
    4. Steady-state kinetics and mechanism of LpxD, the N-acyltransferase of lipid A biosynthesis. Bartling CM, et al. Biochemistry, 2008 May 13. PMID 18422345, Free PMC Article
    5. What is known about the structure and function of the Escherichia coli protein FirA? Dicker IB, et al. Mol Microbiol, 1992 Apr. PMID 1602961

    See all (35) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. The studies highlight key interactions of transient ACP-FabA protein complexes and define the dynamic movements of ACP that facilitate substrate delivery and trigger complex dissociation.
      Title: Crystal structures of acyl carrier protein in complex with two catalytic partners show a dynamic role in cellular metabolism.
    2. Overlay of the LpxA-RJPXD33 structure with E. coli LpxD identified a complementary peptide binding pocket within LpxD and serves as a model for further biochemical characterization of RJPXD33 binding to LpxD.
      Title: Structural basis for the recognition of peptide RJPXD33 by acyltransferases in lipid A biosynthesis.
    3. these data suggest that unphosphorylated NPr decreases lipid A biosynthesis by inhibiting LpxD activity.
      Title: Dephosphorylated NPr of the nitrogen PTS regulates lipid A biosynthesis by direct interaction with LpxD.
    4. N-terminus verified by Edman degradation on mature peptide
      Title: The ilvIH operon of Escherichia coli is positively regulated.
    Submit: New GeneRIF Correction

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables N-acyltransferase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables N-acyltransferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables UDP-3-O-(R-3-hydroxymyristoyl)-glucosamine N-acyltransferase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables UDP-3-O-(R-3-hydroxymyristoyl)-glucosamine N-acyltransferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables acyltransferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables acyltransferase activity, transferring groups other than amino-acyl groups IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables identical protein binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in lipid A biosynthetic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within lipid A biosynthetic process IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in response to antibiotic IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    located_in cytoplasm IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in membrane IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

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    Preferred Names
    UDP-3-O-(3-hydroxymyristoyl)glucosamine N-acyltransferase
    NP_414721.1

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      200971..201996
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_414721.1 UDP-3-O-(3-hydroxymyristoyl)glucosamine N-acyltransferase [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_414721.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A8S7EBK7
      Conserved Domains (1) summary
      PRK00892
      Location:2 → 340
      lpxD; UDP-3-O-[3-hydroxymyristoyl] glucosamine N-acyltransferase; Provisional
    Nucleotide Protein
    Heading Accession and Version

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