napA periplasmic nitrate reductase subunit NapA [Escherichia coli str. K-12 substr. MG1655] - Gene

Summary

Official Symbol: napA
Official Full Name: periplasmic nitrate reductase subunit NapA
Primary source: ECOCYC:EG12067
Locus tag: b2206
See related: ASAP:ABE-0007289
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage: Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as: ECK2198; yojC; yojD; yojE
Summary: Verified Tat substrate: [More information is available at EcoGene: EG12067]. The napA gene encodes the periplasmic nitrate reductase molybdoprotein with an Fe-S center. [More information is available at EcoCyc: EG12067].
NEW: Try the new Gene table
Try the new Transcript table

NC_000913.3 Genomic Context describing neighboring genes

Go to nucleotide: Graphics FASTA GenBank

The NapA signal peptide adopts an alpha-helical conformation upon binding NapD.
Title: Overlapping transport and chaperone-binding functions within a bacterial twin-arginine signal peptide.
N-terminus verified by Edman degradation on mature peptide
Title: The periplasmic nitrate reductase from Escherichia coli: a heterodimeric molybdoprotein with a double-arginine signal sequence and an unusual leader peptide cleavage site.
propose a catalytic cycle for E. coli NapA in which nitrate binds to the Mo(5+) ion and where a stable des-oxo Mo(6+) species may participate.
Title: Spectropotentiometric and structural analysis of the periplasmic nitrate reductase from Escherichia coli.

Submit: New GeneRIF Correction

Products	Interactant	Other Gene	Complex	Source	Pubs	Description

Function	Evidence Code	Pubs
enables 4 iron, 4 sulfur cluster binding	IDA Inferred from Direct Assay more info	PubMed
enables 4 iron, 4 sulfur cluster binding	IEA Inferred from Electronic Annotation more info
enables electron transfer activity	IEA Inferred from Electronic Annotation more info
enables iron ion binding	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables molybdenum ion binding	IBA Inferred from Biological aspect of Ancestor more info
enables molybdenum ion binding	IDA Inferred from Direct Assay more info	PubMed
enables molybdenum ion binding	IEA Inferred from Electronic Annotation more info
enables molybdopterin cofactor binding	IEA Inferred from Electronic Annotation more info
enables nitrate reductase (cytochrome) activity	IEA Inferred from Electronic Annotation more info
enables nitrate reductase activity	IBA Inferred from Biological aspect of Ancestor more info
enables nitrate reductase activity	IDA Inferred from Direct Assay more info	PubMed
enables nitrate reductase activity	IEA Inferred from Electronic Annotation more info
enables nitrate reductase activity	IGI Inferred from Genetic Interaction more info	PubMed
enables oxidoreductase activity	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in Mo-molybdopterin cofactor biosynthetic process	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within anaerobic respiration	IGI Inferred from Genetic Interaction more info	PubMed
involved_in anaerobic respiration	NAS Non-traceable Author Statement more info	PubMed
involved_in cellular respiration	IEA Inferred from Electronic Annotation more info
involved_in nitrate assimilation	IEA Inferred from Electronic Annotation more info
involved_in nitrate assimilation	NAS Non-traceable Author Statement more info	PubMed

Component	Evidence Code	Pubs
is_active_in membrane	IBA Inferred from Biological aspect of Ancestor more info
part_of nitrate reductase complex	IBA Inferred from Biological aspect of Ancestor more info
part_of nitrate reductase complex	IPI Inferred from Physical Interaction more info	PubMed
located_in outer membrane-bounded periplasmic space	IDA Inferred from Direct Assay more info	PubMed
part_of oxidoreductase complex	IEA Inferred from Electronic Annotation more info
located_in periplasmic space	IDA Inferred from Direct Assay more info	PubMed
located_in periplasmic space	IEA Inferred from Electronic Annotation more info

NP_416710.1

NEW Try the new Transcript table

The following sections contain reference sequences that belong to a specific genome build. Explain