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    trkA NAD-binding component of Trk potassium transporters [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 947788, updated on 17-Dec-2024

    Summary

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    Official Symbol
    trkA
    Official Full Name
    NAD-binding component of Trk potassium transporters
    Primary source
    ECOCYC:EG11019
    Locus tag
    b3290
    See related
    ASAP:ABE-0010787
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK3276
    Summary
    Although normally required for Trk activity, overproduction of TrkG or TrkH alone increases K+ transport in a trkA mutant strain (Buurman, 2004). [More information is available at EcoGene: EG11019]. trkA mutants were identified in a kdp background as requiring significantly elevated levels of K+ for growth . trkA encodes part of a K+ transport system . [More information is available at EcoCyc: EG11019].
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    Genomic context

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    Sequence:
    NC_000913.3 (3436518..3437894)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene 10-formyltetrahydrofolate:L-methionyl-tRNA(fMet) N-formyltransferase Neighboring gene 16S rRNA m(5)C967 methyltransferase Neighboring gene large conductance mechanosensitive channel Neighboring gene alternative ribosome-rescue factor A Neighboring gene DNA-binding transcriptional activator ZntR

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Energy coupling to net K+ transport in Escherichia coli K-12. Rhoads DB, et al. J Biol Chem, 1977 Feb 25. PMID 320207
    2. Physical mapping of the K+ transport trkA gene of Escherichia coli and overproduction of the TrkA protein. Hamann A, et al. J Bacteriol, 1987 Jul. PMID 3298214, Free PMC Article
    3. The effects of weak acids on potassium uptake by Escherichia coli K-12 inhibition by low cytoplasmic pH. Bakker EP, et al. Biochim Biophys Acta, 1983 May 5. PMID 6405784
    4. The regulation of potassium fluxes for the adjustment and maintenance of potassium levels in Escherichia coli. Meury J, et al. Eur J Biochem, 1981 Sep. PMID 7042336
    5. Formate increases the F0F1-ATPase activity in Escherichia coli growing on glucose under anaerobic conditions at slightly alkaline pH. Bagramyan K, et al. Biochem Biophys Res Commun, 2003 Jun 27. PMID 12804571

    See all (45) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. although lagging-strand synthesis is still perturbed in holD trkA mutants, the trkA mutation allows HolD-less Pol III HE to resist increased levels of the SOS-induced bypass polymerase DinB
      Title: Mutations Affecting Potassium Import Restore the Viability of the Escherichia coli DNA Polymerase III holD Mutant.
    2. Complementation experiments revealed that Aa-TrkA and Aa-TrkH from A. amylolytica strain N10 restored the ability to grow at low K(+) concentration in E. coli DeltatrkA and DeltatrkG DeltatrkH strains, respectively.
      Title: Characterization and function analysis of a Halo-alkaline-adaptable Trk K+ uptake system in Alkalimonas amylolytica strain N10.
    3. N-terminus verified by Edman degradation on complete protein
      Title: NAD+ binding to the Escherichia coli K(+)-uptake protein TrkA and sequence similarity between TrkA and domains of a family of dehydrogenases suggest a role for NAD+ in bacterial transport.
    4. IIANtr regulates the K+ transporter TrkA
      Title: Escherichia coli enzyme IIANtr regulates the K+ transporter TrkA.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables NAD binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables potassium ion transmembrane transporter activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    Process Evidence Code Pubs
    acts_upstream_of_or_within potassium ion transmembrane transport IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in potassium ion transport IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in potassium ion transport IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    located_in cytoplasmic side of plasma membrane IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in plasma membrane IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

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    Preferred Names
    NAD-binding component of Trk potassium transporters

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      3436518..3437894
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_417748.1 NAD-binding component of Trk potassium transporters [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_417748.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A376FYM1, A0A5D8M313, A0A831GTI0
      Conserved Domains (1) summary
      PRK09496
      Location:1 → 456
      trkA; Trk system potassium transporter TrkA
    Nucleotide Protein
    Heading Accession and Version

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