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    pgaC poly-N-acetyl-D-glucosamine synthase subunit PgaC [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 945606, updated on 3-Dec-2024

    Summary

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    Official Symbol
    pgaC
    Official Full Name
    poly-N-acetyl-D-glucosamine synthase subunit PgaC
    Primary source
    ECOCYC:G6529
    Locus tag
    b1022
    See related
    ASAP:ABE-0003464
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK1012; hmsR; ycdQ
    Summary
    PgaC complements a mutation in the homologous Y. pestis hemin storage gene hmsR. [More information is available at EcoGene: EG13863]. The pgaABCD locus is required for the synthesis of a cell-bound hexosamine-rich polysaccharide, which was identified as a linear polymer of beta-1,6-N-acetylglucosamine residues (PGA), an adhesin essential in biofilm formation . [More information is available at EcoCyc: G6529].
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    Genomic context

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    Sequence:
    NC_000913.3 (1086521..1087846, complement)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene ATP-binding protein PhoH Neighboring gene poly-N-acetyl-D-glucosamine synthase subunit PgaD Neighboring gene poly-beta-1,6-N-acetyl-D-glucosamine N-deacetylase and beta-1,6 glycoside hydrolase Neighboring gene partially deacetylated poly-beta-1,6-N-acetyl-D-glucosamine export outer membrane porin Neighboring gene putative diguanylate cyclase DgcT

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Depolymerization of beta-1,6-N-acetyl-D-glucosamine disrupts the integrity of diverse bacterial biofilms. Itoh Y, et al. J Bacteriol, 2005 Jan. PMID 15601723, Free PMC Article
    2. Allosteric activation of exopolysaccharide synthesis through cyclic di-GMP-stimulated protein-protein interaction. Steiner S, et al. EMBO J, 2013 Feb 6. PMID 23202856, Free PMC Article
    3. HmsT, a protein essential for expression of the haemin storage (Hms+) phenotype of Yersinia pestis. Jones HA, et al. Microbiology (Reading), 1999 Aug. PMID 10463178
    4. Effect of growth conditions on poly-N-acetylglucosamine expression and biofilm formation in Escherichia coli. Cerca N, et al. FEMS Microbiol Lett, 2008 Jun. PMID 18445167
    5. Spatial periodicity of Escherichia coli K-12 biofilm microstructure initiates during a reversible, polar attachment phase of development and requires the polysaccharide adhesin PGA. Agladze K, et al. J Bacteriol, 2005 Dec. PMID 16321928, Free PMC Article

    See all (17) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. R222 of PgaC plays an essential role in c-di-GMP-dependent PgaCD activation
      Title: Allosteric activation of exopolysaccharide synthesis through cyclic di-GMP-stimulated protein-protein interaction.
    2. Mutants lacking the polysaccharide adhesin beta-1,6-N-acetyl-d-glucosamine (DeltapgaC) also exhibited aperiodic cell distribution, but without apparent cell-cell interactions, and were defective in forming permanent attachments.
      Title: Spatial periodicity of Escherichia coli K-12 biofilm microstructure initiates during a reversible, polar attachment phase of development and requires the polysaccharide adhesin PGA.
    Submit: New GeneRIF Correction

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables acetylglucosaminyltransferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables acetylglucosaminyltransferase activity IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    enables acetylglucosaminyltransferase activity RCA
    inferred from Reviewed Computational Analysis
    more info
    		 PubMed 
    enables glycosyltransferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    Process Evidence Code Pubs
    acts_upstream_of_or_within cell adhesion involved in biofilm formation EXP
    Inferred from Experiment
    more info
    		 PubMed 
    involved_in cell adhesion involved in biofilm formation IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in cell adhesion involved in biofilm formation IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within cell adhesion involved in biofilm formation IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in plasma membrane IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in plasma membrane IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in plasma membrane RCA
    inferred from Reviewed Computational Analysis
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    poly-N-acetyl-D-glucosamine synthase subunit PgaC

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      1086521..1087846 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_415541.1 poly-N-acetyl-D-glucosamine synthase subunit PgaC [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_415541.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A376M9E7
      Conserved Domains (1) summary
      PRK11204
      Location:21 → 438
      PRK11204; N-glycosyltransferase; Provisional
    Nucleotide Protein
    Heading Accession and Version

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