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    mutL DNA mismatch repair protein MutL [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 948691, updated on 17-Dec-2024

    Summary

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    Official Symbol
    mutL
    Official Full Name
    DNA mismatch repair protein MutL
    Primary source
    ECOCYC:EG11281
    Locus tag
    b4170
    See related
    ASAP:ABE-0013655
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK4166
    Summary
    MutL DNA-binding is required for methyl-directed mismatch repair (Robertson, 2006). [More information is available at EcoGene: EG11281]. MutL is one of the components of the MutHLS complex. [More information is available at EcoCyc: EG11281].
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    Genomic context

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    Sequence:
    NC_000913.3 (4397412..4399259)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene NAD(P)HX epimerase/NAD(P)HX dehydratase Neighboring gene N(6)-L-threonylcarbamoyladenine synthase, TsaE subunit Neighboring gene N-acetylmuramoyl-L-alanine amidase B Neighboring gene tRNA dimethylallyltransferase Neighboring gene RNA-binding protein Hfq Neighboring gene ribosome rescue factor HflX

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Evidence for ATP-dependent structural rearrangement of nuclease catalytic site in DNA mismatch repair endonuclease MutL. Yamamoto T, et al. J Biol Chem, 2011 Dec 9. PMID 21953455, Free PMC Article
    2. Dominant negative mutator mutations in the mutL gene of Escherichia coli. Aronshtam A, et al. Nucleic Acids Res, 1996 Jul 1. PMID 8692687, Free PMC Article
    3. MutL: conducting the cell's response to mismatched and misaligned DNA. Polosina YY, et al. Bioessays, 2010 Jan. PMID 19953589
    4. Transformation of MutL by ATP binding and hydrolysis: a switch in DNA mismatch repair. Ban C, et al. Cell, 1999 Apr 2. PMID 10199405
    5. Single-molecule analysis reveals the kinetics and physiological relevance of MutL-ssDNA binding. Park J, et al. PLoS One, 2010 Nov 12. PMID 21103398, Free PMC Article

    See all (115) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. MutL binds to 3' resected DNA ends and blocks DNA polymerase access.
      Title: MutL binds to 3' resected DNA ends and blocks DNA polymerase access.
    2. The selection process of licensing a DNA mismatch for repair.
      Title: The selection process of licensing a DNA mismatch for repair.
    3. Responses of DNA Mismatch Repair Proteins to a Stable G-Quadruplex Embedded into a DNA Duplex Structure.
      Title: Responses of DNA Mismatch Repair Proteins to a Stable G-Quadruplex Embedded into a DNA Duplex Structure.
    4. Probing the DNA-binding center of the MutL protein from the Escherichia coli mismatch repair system via crosslinking and Forster resonance energy transfer.
      Title: Probing the DNA-binding center of the MutL protein from the Escherichia coli mismatch repair system via crosslinking and Förster resonance energy transfer.
    5. We further show that MutL is unable to activate the helicase activity of a chimeric UvrD containing the 2B subdomain of the structurally similar Rep helicase. Hence, MutL activation of the monomeric UvrD helicase is regulated specifically by its 2B subdomain.
      Title: UvrD helicase activation by MutL involves rotation of its 2B subdomain.
    6. Observations are consistent with an exonuclease-independent MMR strand excision mechanism that relies on EcMutL-EcUvrD helicase-driven displacement of ssDNA segments between adjacent EcMutH-GATC incisions.
      Title: MutL sliding clamps coordinate exonuclease-independent Escherichia coli mismatch repair.
    7. In Bacillus subtilis, the interaction stimulates the endonuclease activity of MutL and it is critical for DNA mismatch repair. Conversely, disrupting the interaction between Escherichia coli MutL and the b-clamp only causes a mild mutator phenotype. Here, we determined the structures of the regulatory domains of E. coli and B. subtilis MutL bound to their respective b-clamps
      Title: Binding of the regulatory domain of MutL to the sliding β-clamp is species specific.
    8. The assembly of an EcMutS-EcMutL-EcMutH search complex illustrates how sequential stable sliding clamps can modulate one-dimensional diffusion mechanics along the DNA to direct mismatch repair
      Title: Cascading MutS and MutL sliding clamps control DNA diffusion to activate mismatch repair.
    9. The structure captures MutS in the sliding clamp conformation, where tilting of the MutS subunits across each other pushes DNA into a new channel, and reorientation of the connector domain creates an interface for MutL with both MutS subunits.
      Title: MutS/MutL crystal structure reveals that the MutS sliding clamp loads MutL onto DNA.
    10. In this review, we describe biochemical, biophysical and structural analyses that have clarified how MutL aids at discriminating the newly synthesized strand from its template and marking it for removal. [review]
      Title: Insights from a decade of biophysical studies on MutL: Roles in strand discrimination and mismatch removal.
    Submit: New GeneRIF Correction See all GeneRIFs (32)

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables ATP binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables ATP binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables ATP hydrolysis activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables ATP hydrolysis activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables ATP hydrolysis activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables ATP hydrolysis activity IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    enables ATP-dependent DNA damage sensor activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables DNA binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables identical protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables mismatched DNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in DNA repair IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in mismatch repair IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in mismatch repair IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in mismatch repair IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in mismatch repair IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in mismatch repair involved in maintenance of fidelity involved in DNA-dependent DNA replication IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in nucleotide-excision repair, DNA duplex unwinding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    acts_upstream_of_or_within regulation of DNA recombination IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in regulation of DNA recombination NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    Component Evidence Code Pubs
    part_of mismatch repair complex IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    part_of mismatch repair complex IC
    Inferred by Curator
    more info
    		 PubMed 
    part_of mismatch repair complex IEA
    Inferred from Electronic Annotation
    more info
    		  
    part_of mismatch repair complex NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    part_of single-stranded DNA-dependent ATP-dependent DNA helicase complex IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    DNA mismatch repair protein MutL

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      4397412..4399259
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_418591.1 DNA mismatch repair protein MutL [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_418591.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A8S7X036
      Conserved Domains (1) summary
      PRK00095
      Location:1 → 613
      mutL; DNA mismatch repair endonuclease MutL
    Nucleotide Protein
    Heading Accession and Version

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