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    rep ATP-dependent DNA helicase Rep [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 948292, updated on 17-Dec-2024

    Summary

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    Official Symbol
    rep
    Official Full Name
    ATP-dependent DNA helicase Rep
    Primary source
    ECOCYC:EG10837
    Locus tag
    b3778
    See related
    ASAP:ABE-0012344
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    dasC; ECK3770; mbrA; mmrA
    Summary
    Rep is an ATP-dependent helicase that is required for genomic replication in Escherichia coli and some bacteriophages . [More information is available at EcoCyc: EG10837].
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    Genomic context

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    Sequence:
    NC_000913.3 (3960677..3962698)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene peptidyl-prolyl cis-trans isomerase C Neighboring gene pseudo Neighboring gene guanosine-5'-triphosphate,3'-diphosphate phosphatase Neighboring gene ATP-dependent RNA helicase RhlB

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Complexes of Rep protein with ATP and DNA as a basis for helicase action. Arai N, et al. J Biol Chem, 1981 May 25. PMID 6112227
    2. Initiation and re-initiation of DNA unwinding by the Escherichia coli Rep helicase. Ha T, et al. Nature, 2002 Oct 10. PMID 12374984
    3. E. coli Rep oligomers are required to initiate DNA unwinding in vitro. Cheng W, et al. J Mol Biol, 2001 Jul 6. PMID 11428893
    4. A two-site kinetic mechanism for ATP binding and hydrolysis by E. coli Rep helicase dimer bound to a single-stranded oligodeoxynucleotide. Hsieh J, et al. J Mol Biol, 1999 Apr 30. PMID 10329141
    5. Kinetic mechanism for the sequential binding of two single-stranded oligodeoxynucleotides to the Escherichia coli Rep helicase dimer. Bjornson KP, et al. Biochemistry, 1998 Jan 20. PMID 9454579

    See all (82) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Single-molecule visualization of stalled replication-fork rescue by the Escherichia coli Rep helicase.
      Title: Single-molecule visualization of stalled replication-fork rescue by the Escherichia coli Rep helicase.
    2. Regulation of E. coli Rep helicase activity by PriC.
      Title: Regulation of E. coli Rep helicase activity by PriC.
    3. E. coli Rep helicase and RecA recombinase unwind G4 DNA and are important for resistance to G4-stabilizing ligands.
      Title: E. coli Rep helicase and RecA recombinase unwind G4 DNA and are important for resistance to G4-stabilizing ligands.
    4. Findings suggest two Rep-replisome populations in vivo: one continually associating with DnaB then translocating away to aid nucleoprotein barrier removal ahead of the fork, another assisting PriC-dependent reloading of DnaB if replisome progression fails.
      Title: Single-molecule live cell imaging of Rep reveals the dynamic interplay between an accessory replicative helicase and the replisome.
    5. Rep and UvrD helicases operate on DNA at the replication fork whereas DinG helicase acts via a different mechanism
      Title: Direct removal of RNA polymerase barriers to replication by accessory replicative helicases.
    6. We provide a quantitative model of the factors that enhance unwinding processivity. Our work sheds light on the mechanisms of regulation of unwinding by Rep-like helicases.
      Title: Regulation of Rep helicase unwinding by an auto-inhibitory subdomain.
    7. Data show that single-stranded DNA binding proteins (SSB)-interacting proteins RecO, RecG, PriA, and accessory helicase Rep do not correlate with replication at relication forks.
      Title: Are the SSB-Interacting Proteins RecO, RecG, PriA and the DnaB-Interacting Protein Rep Bound to Progressing Replication Forks in Escherichia coli?
    8. The authors demonstrate that Pol IV interacts in vitro with Rep DNA helicase and that this interaction enhances Rep's helicase activity.
      Title: Escherichia coli Rep DNA helicase and error-prone DNA polymerase IV interact physically and functionally.
    9. After replication blockage by an inverted rrn, Rep in conjunction with DinG or UvrD removes RNA polymerase, a task that is fulfilled in its absence by the SOS-induced DinG and UvrD helicases.
      Title: The helicases DinG, Rep and UvrD cooperate to promote replication across transcription units in vivo.
    10. N-terminus verified by Edman degradation on mature peptide
      Title: Escherichia coli rep gene: identification of the promoter and N terminus of the rep protein.
    Submit: New GeneRIF Correction See all GeneRIFs (15)

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables 3'-5' DNA helicase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables 3'-5' DNA helicase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables ATP binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables ATP hydrolysis activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables ATP-dependent H2AZ histone chaperone activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables ATP-dependent H3-H4 histone complex chaperone activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables DNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables DNA clamp loader activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables DNA helicase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables DNA helicase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables chromatin extrusion motor activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables cohesin loader activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables double-stranded DNA helicase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables forked DNA-dependent helicase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables four-way junction helicase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables helicase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables hydrolase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables isomerase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables protein homodimerization activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables single-stranded 3'-5' DNA helicase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables single-stranded DNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in DNA replication IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in DNA replication, synthesis of primer NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    acts_upstream_of_or_within DNA unwinding involved in DNA replication IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in DNA unwinding involved in DNA replication IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in DNA unwinding involved in DNA replication NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    acts_upstream_of_or_within bacterial-type DNA replication IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in chromatin looping IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in chromatin remodeling IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in recombinational repair IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in replication fork processing NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    acts_upstream_of_or_within response to radiation IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    part_of DnaB-DnaC-Rep-PriC complex NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    part_of cytoplasmic replisome EXP
    Inferred from Experiment
    more info
    		 PubMed 
    is_active_in cytosol IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    part_of primosome complex NAS
    Non-traceable Author Statement
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    ATP-dependent DNA helicase Rep

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      3960677..3962698
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. YP_026251.1 ATP-dependent DNA helicase Rep [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for YP_026251.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A6M9X1W7
      Conserved Domains (1) summary
      PRK10919
      Location:1 → 672
      PRK10919; ATP-dependent DNA helicase Rep; Provisional
    Nucleotide Protein
    Heading Accession and Version

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