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    aceK isocitrate dehydrogenase kinase/phosphatase [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 944797, updated on 3-Dec-2024

    Summary

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    Official Symbol
    aceK
    Official Full Name
    isocitrate dehydrogenase kinase/phosphatase
    Primary source
    ECOCYC:EG10026
    Locus tag
    b4016
    See related
    ASAP:ABE-0013131
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK4008
    Summary
    Context determines which enzyme name is used, the kinase or the phosphatase. [More information is available at EcoCyc: EG10026].
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    Genomic context

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    Sequence:
    NC_000913.3 (4218596..4220332)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene malate synthase A Neighboring gene isocitrate lyase Neighboring gene ncRNA Neighboring gene regulator of acetyl CoA synthetase Neighboring gene DNA-binding transcriptional repressor IclR Neighboring gene cobalamin-dependent methionine synthase

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Photoaffinity labelling shows that Escherichia coli isocitrate dehydrogenase kinase/phosphatase contains a single ATP-binding site. Varela I, et al. FEBS Lett, 1988 Apr 25. PMID 2834232
    2. Isocitrate dehydrogenase kinase/phosphatase. Kinetic characteristics of the wild-type and two mutant proteins. Miller SP, et al. J Biol Chem, 1996 Aug 9. PMID 8702587
    3. Escherichia coli isocitrate dehydrogenase kinase/phosphatase. Overproduction and kinetics of interaction with its substrates by using intrinsic fluorescence and fluorescent nucleotide analogues. Rittinger K, et al. Eur J Biochem, 1996 Apr 1. PMID 8620880
    4. The isocitrate dehydrogenase phosphorylation cycle: regulation and enzymology. LaPorte DC. J Cell Biochem, 1993 Jan. PMID 8381789
    5. Determination of flux through the branch point of two metabolic cycles. The tricarboxylic acid cycle and the glyoxylate shunt. Walsh K, et al. J Biol Chem, 1984 Aug 10. PMID 6378912

    See all (50) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Characterization of metal binding of bifunctional kinase/phosphatase AceK and implication in activity modulation.
      Title: Characterization of metal binding of bifunctional kinase/phosphatase AceK and implication in activity modulation.
    2. highly stringent AceK binding sites on isocitrate dehydrogenase
      Title: Structural basis of the substrate specificity of bifunctional isocitrate dehydrogenase kinase/phosphatase.
    3. In this study, AceK was successfully cocrystallized in complex with its intact protein substrate, isocitrate dehydrogenase, in the presence of ATP.
      Title: Purification, crystallization and preliminary X-ray analysis of bifunctional isocitrate dehydrogenase kinase/phosphatase in complex with its substrate, isocitrate dehydrogenase, from Escherichia coli.
    4. N-terminus verified by Edman degradation on mature peptide
      Title: Nucleotide sequence of aceK, the gene encoding isocitrate dehydrogenase kinase/phosphatase.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables AMP binding IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables AMP binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables ATP binding IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables ATP binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables ATP binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables [isocitrate dehydrogenase (NADP+)] kinase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables [isocitrate dehydrogenase (NADP+)] kinase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables [isocitrate dehydrogenase (NADP+)] kinase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables hydrolase activity, acting on ester bonds IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables magnesium ion binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables manganese ion binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables phosphatase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables phosphoprotein phosphatase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables phosphoprotein phosphatase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables protein serine/threonine kinase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables protein serine/threonine kinase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein serine/threonine phosphatase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in glucose metabolic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within glyoxylate cycle IC
    Inferred by Curator
    more info
    		 PubMed 
    involved_in glyoxylate cycle IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within tricarboxylic acid cycle IC
    Inferred by Curator
    more info
    		 PubMed 
    involved_in tricarboxylic acid cycle IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    located_in cytoplasm IC
    Inferred by Curator
    more info
    		 PubMed 
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

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    Preferred Names
    isocitrate dehydrogenase kinase/phosphatase
    NP_418440.1

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      4218596..4220332
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_418440.1 isocitrate dehydrogenase kinase/phosphatase [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_418440.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A376FSU2, A0A3K1A8W6
      Conserved Domains (1) summary
      COG4579
      Location:1 → 578
      AceK; Isocitrate dehydrogenase kinase/phosphatase [Signal transduction mechanisms]
    Nucleotide Protein
    Heading Accession and Version

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