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    rtcB RNA-splicing ligase [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 947929, updated on 17-Dec-2024

    Summary

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    Official Symbol
    rtcB
    Official Full Name
    RNA-splicing ligase
    Primary source
    ECOCYC:G7751
    Locus tag
    b3421
    See related
    ASAP:ABE-0011170
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK3407; yhgL
    Summary
    RtcB has similarity to proteins found in Eucarya, Bacteria, and Archaea . [More information is available at EcoCyc: G7751].
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    Genomic context

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    Sequence:
    NC_000913.3 (3556853..3558079, complement)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene DNA-binding transcriptional activator MalT Neighboring gene RNA 3'-terminal phosphate cyclase Neighboring gene DNA-binding transcriptional activator RtcR Neighboring gene pseudo Neighboring gene rhomboid protease GlpG

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Rewriting the rules for end joining via enzymatic splicing of DNA 3'-PO4 and 5'-OH ends. Das U, et al. Proc Natl Acad Sci U S A, 2013 Dec 17. PMID 24218597, Free PMC Article
    2. Sequential rescue and repair of stalled and damaged ribosome by bacterial PrfH and RtcB. Tian Y, et al. Proc Natl Acad Sci U S A, 2022 Jul 19. PMID 35858322, Free PMC Article
    3. The RNA ligase RtcB reverses MazF-induced ribosome heterogeneity in Escherichia coli. Temmel H, et al. Nucleic Acids Res, 2017 May 5. PMID 27789694, Free PMC Article
    4. RNA ligase RtcB splices 3'-phosphate and 5'-OH ends via covalent RtcB-(histidinyl)-GMP and polynucleotide-(3')pp(5')G intermediates. Chakravarty AK, et al. Proc Natl Acad Sci U S A, 2012 Apr 17. PMID 22474365, Free PMC Article
    5. Characterization of the Escherichia coli RNA 3'-terminal phosphate cyclase and its sigma54-regulated operon. Genschik P, et al. J Biol Chem, 1998 Sep 25. PMID 9738023

    See all (12) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Sequential rescue and repair of stalled and damaged ribosome by bacterial PrfH and RtcB.
      Title: Sequential rescue and repair of stalled and damaged ribosome by bacterial PrfH and RtcB.
    2. our findings not only provide a physiological function for the RNA ligase RtcB in bacteria but highlight the reversibility of ribosome heterogeneity, a crucial but hitherto undescribed concept for translational regulation.
      Title: The RNA ligase RtcB reverses MazF-induced ribosome heterogeneity in Escherichia coli.
    3. Data show that RtcB seals broken RNAs with 3'-phosphate and 5'-OH ends.
      Title: RNA ligase RtcB splices 3'-phosphate and 5'-OH ends via covalent RtcB-(histidinyl)-GMP and polynucleotide-(3')pp(5')G intermediates.
    4. RtcB, a novel RNA ligase, can catalyze tRNA splicing and HAC1 mRNA splicing in vivo
      Title: RtcB, a novel RNA ligase, can catalyze tRNA splicing and HAC1 mRNA splicing in vivo.
    Submit: New GeneRIF Correction

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables DNA ligase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables GTP binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables RNA ligase (GTP) activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables ligase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables manganese ion binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in DNA damage response IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in DNA repair IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in DNA repair IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in RNA processing IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in RNA processing IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within RNA repair EXP
    Inferred from Experiment
    more info
    		 PubMed 
    involved_in RNA repair IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in RNA repair IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in RNA repair TAS
    Traceable Author Statement
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    RNA-splicing ligase
    NP_417879.1

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      3556853..3558079 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_417879.1 RNA-splicing ligase [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_417879.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A376CXY1, A0A8T6Q6W5
      Conserved Domains (1) summary
      pfam01139
      Location:29 → 408
      RtcB; tRNA-splicing ligase RtcB
    Nucleotide Protein
    Heading Accession and Version

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