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    csrA carbon storage regulator [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 947176, updated on 3-Dec-2024

    Summary

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    Official Symbol
    csrA
    Official Full Name
    carbon storage regulator
    Primary source
    ECOCYC:EG11447
    Locus tag
    b2696
    See related
    ASAP:ABE-0008866
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK2691; zfiA
    Summary
    CsrA translationally represses the GGDEF diguanylate cyclase enzymes YdeH and YcdT, which are needed for normal motility (Jonas, 2008). [More information is available at EcoGene: EG11447]. CsrA is a regulator of carbohydrate metabolism that affects glycogen biosynthesis , glycogen degradation , gluconeogenesis , and glycolysis . [More information is available at EcoCyc: EG11447].
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    Genomic context

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    Sequence:
    NC_000913.3 (2818961..2819146, complement)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene tRNA-Arg Neighboring gene tRNA-Ser Neighboring gene alanine--tRNA ligase/DNA-binding transcriptional repressor Neighboring gene RecA inhibitor RecX

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Conditional essentiality of the csrA gene in Escherichia coli. Timmermans J, et al. J Bacteriol, 2009 Mar. PMID 19103924, Free PMC Article
    2. A novel endogenous induction of ColE7 expression in a csrA mutant of Escherichia coli. Chang HW, et al. Curr Microbiol, 2013 Apr. PMID 23247769
    3. Posttranscriptional repression of the cel gene of the ColE7 operon by the RNA-binding protein CsrA of Escherichia coli. Yang TY, et al. Nucleic Acids Res, 2010 Jul. PMID 20378712, Free PMC Article
    4. Regulation of Salmonella enterica serovar typhimurium invasion genes by csrA. Altier C, et al. Infect Immun, 2000 Dec. PMID 11083797, Free PMC Article
    5. Thymineless death is inhibited by CsrA in Escherichia coli lacking the SOS response. Hamilton HM, et al. DNA Repair (Amst), 2013 Nov. PMID 24075571, Free PMC Article

    See all (75) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Two Polyketides Intertwined in Complex Regulation: Posttranscriptional CsrA-Mediated Control of Colibactin and Yersiniabactin Synthesis in Escherichia coli.
      Title: Two Polyketides Intertwined in Complex Regulation: Posttranscriptional CsrA-Mediated Control of Colibactin and Yersiniabactin Synthesis in Escherichia coli.
    2. CsrA-Mediated Translational Activation of ymdA Expression in Escherichia coli.
      Title: CsrA-Mediated Translational Activation of ymdA Expression in Escherichia coli.
    3. CsrA was found to regulate cellular iron levels and support growth by repressing the expression of FtnB and Bfr.
      Title: Regulation of Iron Storage by CsrA Supports Exponential Growth of Escherichia coli.
    4. findings expand the scope of pathways affected by the csrA regulon and emphasize its importance as a global regulator.
      Title: Metabolome and transcriptome-wide effects of the carbon storage regulator A in enteropathogenic Escherichia coli.
    5. Based on expression studies with site-directed mutants, an OFF-ON-OFF toggle model is proposed that results in transient translation of SepL and EspA filament assembly. Under this model, the nascent mRNA is translationally off, before being activated by CsrA, and then repressed by Hfq and Spot42.
      Title: An RNA-dependent mechanism for transient expression of bacterial translocation filaments.
    6. The authors propose that in both Azotobacter vinelandii and Escherichia coli, RppH activity plays a role in the expression of the translational regulator protein RsmA/CsrA.
      Title: The pyrophosphohydrolase RppH is involved in the control of RsmA/CsrA expression in Azotobacter vinelandii and Escherichia coli.
    7. Findings indicate the integration of CsrA throughout the regulatory network, where it orchestrates vast effects on gene expression.
      Title: Global role of the bacterial post-transcriptional regulator CsrA revealed by integrated transcriptomics.
    8. findings show that CsrA employs a novel regulatory mechanism to repress translation of iraD; CsrA binds to four sites in the iraD leader transcript but does not directly occlude ribosome binding to the iraD SD sequence; instead, CsrA represses translation of a short open reading frame encoded upstream of iraD, causing repression of iraD translation
      Title: Translational Repression of the RpoS Antiadapter IraD by CsrA Is Mediated via Translational Coupling to a Short Upstream Open Reading Frame.
    9. Study provides evidence that CsrA is a global positive regulator of mRNA stability in Escherichia coli and suggests that this mechanism might contribute to metabolic adaptation by regulating expression of genes involved in carbon metabolism and transport independently of transcriptional regulation.
      Title: The Csr system regulates genome-wide mRNA stability and transcription and thus gene expression in Escherichia coli.
    10. Data show that CsrA binds directly to the 5' end of the transcript encoding AcrAB.
      Title: CsrA maximizes expression of the AcrAB multidrug resistance transporter.
    Submit: New GeneRIF Correction See all GeneRIFs (33)

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables RNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables mRNA 5'-UTR binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in mRNA catabolic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in mRNA stabilization IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in negative regulation of translation IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in negative regulation of translational initiation IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within positive regulation of translational initiation IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in positive regulation of translational initiation IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within positive regulation of translational initiation IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in regulation of carbohydrate metabolic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in regulation of translation IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  
    is_active_in cytosol IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    carbon storage regulator

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      2818961..2819146 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_417176.1 carbon storage regulator [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_417176.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A485JE11
      Conserved Domains (1) summary
      PRK01712
      Location:1 → 60
      PRK01712; carbon storage regulator CsrA
    Nucleotide Protein
    Heading Accession and Version

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