U.S. flag

An official website of the United States government

Format

Send to:

Choose Destination

Links from GEO Profiles

    • Showing Current items.

    nanA N-acetylneuraminate lyase [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 947742, updated on 3-Dec-2024

    Summary

    Go to the top of the page Help
    Official Symbol
    nanA
    Official Full Name
    N-acetylneuraminate lyase
    Primary source
    ECOCYC:EG10637
    Locus tag
    b3225
    See related
    ASAP:ABE-0010579
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK3214
    Summary
    N-acetylneuraminate lyase is the first enzyme in the pathway for degradation of sialic acid (N-acetylneuraminate, or NeuNAc) . [More information is available at EcoCyc: EG10637].
    NEW
    Try the new Gene table
    Try the new Transcript table

    Genomic context

    Go to the top of the page Help
    Sequence:
    NC_000913.3 (3372683..3373576, complement)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene N-acetylmannosamine kinase Neighboring gene putative N-acetylmannosamine-6-phosphate 2-epimerase Neighboring gene N-acetylneuraminate:H(+) symporter Neighboring gene NanR Neighboring gene putative transporter DcuD

    Genomic regions, transcripts, and products

    Go to the top of the page Help
    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

    Go to the top of the pageHelp

    Related articles in PubMed

    1. Modulation of substrate specificities of D-sialic acid aldolase through single mutations of Val-251. Chou CY, et al. J Biol Chem, 2011 Apr 22. PMID 21270125, Free PMC Article
    2. High-level production and purification of Escherichia coli N-acetylneuraminic acid aldolase (EC 4.1.3.3). Lilley GG, et al. Protein Expr Purif, 1992 Oct. PMID 1458058
    3. Purification, crystallization and characterization of N-acetylneuraminate lyase from Escherichia coli. Aisaka K, et al. Biochem J, 1991 Jun 1. PMID 1646603, Free PMC Article
    4. Inhibition of N-acetylneuraminate lyase by N-acetyl-4-oxo-D-neuraminic acid. Gross HJ, et al. FEBS Lett, 1988 May 9. PMID 2896604
    5. Genetic and molecular analyses of Escherichia coli N-acetylneuraminate lyase gene. Kawakami B, et al. J Bacteriol, 1986 Jul. PMID 3522555, Free PMC Article

    See all (31) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. analysis of modulation of substrate specificities of D-sialic acid aldolase through single mutations of Val-251
      Title: Modulation of substrate specificities of D-sialic acid aldolase through single mutations of Val-251.
    2. A comparison of mutant and wild-type nanA structures, in combination with the specificity profiles of the variants, reveals subtle differences that explain the details of the substrate specificity changes.
      Title: Structural insights into substrate specificity in variants of N-acetylneuraminic Acid lyase produced by directed evolution.
    3. Results describe the structure of a mutant variant of Escherichia coli N-acetyl-d-neuraminic acid lyase (NAL), E192N, in complex with pyruvate in a new crystal form.
      Title: Structure of an Escherichia coli N-acetyl-D-neuraminic acid lyase mutant, E192N, in complex with pyruvate at 1.45 angstrom resolution.
    4. the dimer-dimer interface of NAL is intolerant to mutation and essential for functional expression.
      Title: The quaternary structure of Escherichia coli N-acetylneuraminate lyase is essential for functional expression.
    5. N-terminus verified by Edman degradation on complete protein
      Title: Complete nucleotide sequence of the E. coli N-acetylneuraminate lyase.
    Submit: New GeneRIF Correction

    Interactions

    Go to the top of the page Help
    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

    Go to the top of the page Help

    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables N-acetylneuraminate lyase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables N-acetylneuraminate lyase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables N-acetylneuraminate lyase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables identical protein binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables lyase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in N-acetylneuraminate catabolic process IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in N-acetylneuraminate catabolic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in N-acetylneuraminate catabolic process IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in carbohydrate metabolic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within_positive_effect single-species biofilm formation IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  
    is_active_in cytosol IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

    Go to the top of the page Help
    Preferred Names
    N-acetylneuraminate lyase

    NCBI Reference Sequences (RefSeq)

    Go to the top of the page Help

    NEW Try the new Transcript table

    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      3372683..3373576 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_417692.1 N-acetylneuraminate lyase [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_417692.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A376JRE7, A0A5B9ANI8, A0A7D7EZH8
      Conserved Domains (1) summary
      TIGR00683
      Location:5 → 297
      nanA; N-acetylneuraminate lyase
    Nucleotide Protein
    Heading Accession and Version

    Gene LinkOut

    The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.