RPN8 proteasome regulatory particle lid subunit RPN8 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: RPN8
Official Full Name: proteasome regulatory particle lid subunit RPN8
Primary source: SGD:S000005787
Locus tag: YOR261C
See related: AllianceGenome:SGD:S000005787; FungiDB:YOR261C; VEuPathDB:YOR261C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Predicted to enable metallopeptidase activity. Predicted to be involved in proteasome-mediated ubiquitin-dependent protein catabolic process. Located in proteasome storage granule. Part of proteasome regulatory particle, lid subcomplex. Orthologous to human PSMD7 (proteasome 26S subunit, non-ATPase 7). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See RPN8 in Genome Data Viewer

Location:: chromosome: XV

Exon count:: 1

Sequence:: Chromosome: XV; NC_001147.6 (815915..816931, complement)

Chromosome XV - NC_001147.6 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001147.6

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

A key role for rpn8 in 26S proteasome
Title: Base-CP proteasome can serve as a platform for stepwise lid formation.
Rpn11-Rpn8 interaction occurs via two distinct interfaces; Rpn11 lacks a conserved surface to bind the ubiquitin Ile44 patch
Title: Structure of the Rpn11-Rpn8 dimer reveals mechanisms of substrate deubiquitination during proteasomal degradation.
Data show that proteasome subunits Rpt1, Rpt4, Rpn8, Rpn12, Pre6, and Pre10 are recruited to GAL10 rapidly upon galactose induction.
Title: Similar temporal and spatial recruitment of native 19S and 20S proteasome subunits to transcriptionally active chromatin.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables metallopeptidase activity	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in proteasome-mediated ubiquitin-dependent protein catabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in ubiquitin-dependent protein catabolic process	TAS Traceable Author Statement more info	PubMed

Component	Evidence Code	Pubs
part_of proteasome complex	IBA Inferred from Biological aspect of Ancestor more info
part_of proteasome complex	IEA Inferred from Electronic Annotation more info
part_of proteasome regulatory particle	IEA Inferred from Electronic Annotation more info
part_of proteasome regulatory particle, lid subcomplex	IDA Inferred from Direct Assay more info	PubMed
located_in proteasome storage granule	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: proteasome regulatory particle lid subunit RPN8

NP_014904.3

Essential non-ATPase regulatory subunit of the 26S proteasome; has similarity to the human p40 proteasomal subunit and to another S. cerevisiae regulatory subunit, Rpn11p

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001147.6 Reference assembly

Range

815915..816931 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001183680.3 → NP_014904.3 TPA: proteasome regulatory particle lid subunit RPN8 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_014904.3

Status: REVIEWED

UniProtKB/Swiss-Prot

D6W2W2, Q08723, Q6Q5I7

UniProtKB/TrEMBL

B3LJS9, B5VSE2, C8ZH12, N1P3K9

Conserved Domains (1) summary

cd08062
Location:6 → 303

MPN_RPN7_8; Mpr1p, Pad1p N-terminal (MPN) domains without catalytic isopeptidase activity, found in 19S proteasomal subunits Rpn7 and Rpn8