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    POL31 DNA-directed DNA polymerase delta subunit POL31 [ Saccharomyces cerevisiae S288C ]

    Gene ID: 853462, updated on 9-Dec-2024

    Summary

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    Official Symbol
    POL31
    Official Full Name
    DNA-directed DNA polymerase delta subunit POL31
    Primary source
    SGD:S000003766
    Locus tag
    YJR006W
    See related
    AllianceGenome:SGD:S000003766; FungiDB:YJR006W; VEuPathDB:YJR006W
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Saccharomyces cerevisiae S288C (strain: S288C)
    Lineage
    Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
    Also known as
    HUS2; HYS2; SDP5
    Summary
    Enables DNA-directed DNA polymerase activity. Involved in DNA replication, removal of RNA primer and RNA-templated DNA biosynthetic process. Located in cytosol and nucleus. Part of zeta DNA polymerase complex. Used to study cancer. Orthologous to human POLD2 (DNA polymerase delta 2, accessory subunit). [provided by Alliance of Genome Resources, Dec 2024]
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    Genomic context

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    See POL31 in Genome Data Viewer
    Location:
    chromosome: X
    Exon count:
    1
    Sequence:
    Chromosome: X; NC_001142.9 (449202..450665)

    Chromosome X - NC_001142.9Genomic Context describing neighboring genes Neighboring gene Apl1p Neighboring gene Lso1p Neighboring gene translation initiation factor eIF2 subunit alpha Neighboring gene Mho1p

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_001142.9

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. HYS2, an essential gene required for DNA replication in Saccharomyces cerevisiae. Sugimoto K, et al. Nucleic Acids Res, 1995 Sep 11. PMID 7567461, Free PMC Article
    2. The second subunit of DNA polymerase III (delta) is encoded by the HYS2 gene in Saccharomyces cerevisiae. Hashimoto K, et al. Nucleic Acids Res, 1998 Jan 15. PMID 9421503, Free PMC Article
    3. Structure of eukaryotic DNA polymerase δ bound to the PCNA clamp while encircling DNA. Zheng F, et al. Proc Natl Acad Sci U S A, 2020 Dec 1. PMID 33203675, Free PMC Article
    4. DNA polymerase delta, RFC and PCNA are required for repair synthesis of large looped heteroduplexes in Saccharomyces cerevisiae. Corrette-Bennett SE, et al. Nucleic Acids Res, 2004. PMID 15576353, Free PMC Article
    5. Involvement of the yeast DNA polymerase delta in DNA repair in vivo. Giot L, et al. Genetics, 1997 Aug. PMID 9258670, Free PMC Article

    See all (83) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. DNA polymerase delta: A single Pol31 polymorphism suppresses the strain background-specific lethality of Pol32 inactivation in Saccharomyces cerevisiae.
      Title: DNA polymerase δ: A single Pol31 polymorphism suppresses the strain background-specific lethality of Pol32 inactivation in Saccharomyces cerevisiae.
    2. Structure and mechanism of B-family DNA polymerase zeta specialized for translesion DNA synthesis.
      Title: Structure and mechanism of B-family DNA polymerase ζ specialized for translesion DNA synthesis.
    3. The structure reveals an unexpected arrangement in which the regulatory subunits (Pol31 and Pol32) lie next to the exonuclease domain of Pol3 but do not engage the DNA.
      Title: Cryo-EM structure and dynamics of eukaryotic DNA polymerase δ holoenzyme.
    4. Pol31 and Pol32 subunits of yeast DNA polymerase delta are also essential subunits of DNA polymerase zeta.
      Title: Pol31 and Pol32 subunits of yeast DNA polymerase δ are also essential subunits of DNA polymerase ζ.
    5. Loss of the last four amino acids at the C-terminal end of Pol3 impairs the interaction with Pol31.
      Title: Stable interactions between DNA polymerase δ catalytic and structural subunits are essential for efficient DNA repair.
    6. propose an orientation for the accessory Pol31-Pol32N subunits relative to the Pol3 catalytic core that best agrees with the experimental scattering profile
      Title: Structural insights into yeast DNA polymerase delta by small angle X-ray scattering.
    7. study reveals that Pol31 engages in crosstalk with the other Poldelta subunits, as well as with Mgs1, Rad18 and PCNA; some of the Pol31 regions that are important for such functional cooperations are identified
      Title: Mgs1 and Rad18/Rad5/Mms2 are required for survival of Saccharomyces cerevisiae mutants with novel temperature/cold sensitive alleles of the DNA polymerase delta subunit, Pol31.
    Submit: New GeneRIF Correction

    Pathways from PubChem

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    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by SGD

    Function Evidence Code Pubs
    enables DNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables DNA polymerase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables DNA-directed DNA polymerase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables DNA-directed DNA polymerase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    contributes_to DNA-directed DNA polymerase activity TAS
    Traceable Author Statement
    more info
    		 PubMed 
    contributes_to single-stranded DNA 3'-5' DNA exonuclease activity TAS
    Traceable Author Statement
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in DNA metabolic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in DNA replication IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in DNA replication, removal of RNA primer IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in DNA strand elongation involved in DNA replication IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in RNA-templated DNA biosynthetic process IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in base-excision repair TAS
    Traceable Author Statement
    more info
    		 PubMed 
    involved_in lagging strand elongation TAS
    Traceable Author Statement
    more info
    		 PubMed 
    involved_in leading strand elongation TAS
    Traceable Author Statement
    more info
    		 PubMed 
    involved_in mismatch repair TAS
    Traceable Author Statement
    more info
    		 PubMed 
    involved_in nucleotide-excision repair TAS
    Traceable Author Statement
    more info
    		 PubMed 
    involved_in postreplication repair TAS
    Traceable Author Statement
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    part_of delta DNA polymerase complex IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    part_of delta DNA polymerase complex IEA
    Inferred from Electronic Annotation
    more info
    		  
    part_of delta DNA polymerase complex TAS
    Traceable Author Statement
    more info
    		 PubMed 
    located_in nucleus IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in nucleus IEA
    Inferred from Electronic Annotation
    more info
    		  
    part_of zeta DNA polymerase complex IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    DNA-directed DNA polymerase delta subunit POL31
    NP_012539.1
    • Subunit of DNA polymerase delta (polymerase III); essential for cell viability; involved in DNA replication and DNA repair; forms a complex with Rev3p, Rev7p and Pol32p; relocalizes to the cytosol in response to hypoxia

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_001142.9 Reference assembly

      Range
      449202..450665
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_001181663.1 → NP_012539.1  TPA: DNA-directed DNA polymerase delta subunit POL31 [Saccharomyces cerevisiae S288C]

      See identical proteins and their annotated locations for NP_012539.1

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      D6VWI1, P46957
      UniProtKB/TrEMBL
      D3UF73, N1P1G3
      Conserved Domains (1) summary
      COG1311
      Location:5 → 487
      HYS2; Archaeal DNA polymerase II, small subunit/DNA polymerase delta, subunit B [Replication, recombination and repair]
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    P46957.1

    Gene LinkOut

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