pcnB poly(A) polymerase I [Escherichia coli str. K-12 substr. MG1655] - Gene

Summary

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Official Symbol: pcnB
Official Full Name: poly(A) polymerase I
Primary source: ECOCYC:EG10690
Locus tag: b0143
See related: ASAP:ABE-0000493
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage: Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as: ECK0142
Summary: AUU start proven by mutagenesis analysis. [More information is available at EcoGene: EG10690]. Poly(A) polymerase I is responsible for the polyadenylation of 3' ends of RNA molecules. [More information is available at EcoCyc: EG10690].
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Genomic context

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Sequence:: NC_000913.3 (157729..159126, complement)

NC_000913.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Poly(A)polymerase I participates in the ability of the bacteria to adapt to and survive detrimental conditions by constantly monitoring and adjusting to its environment.
Title: Role of polyadenylation in regulation of the flagella cascade and motility in Escherichia coli.
THe data demonstrates that regulation of PAP I is critical not for preventing the decay of mRNAs, but rather for maintaining normal levels of functional tRNAs and protein synthesis in E. coli, a function for polyadenylation that has not been observed previously in any organism.
Title: Deregulation of poly(A) polymerase I in Escherichia coli inhibits protein synthesis and leads to cell death.
Characterization of RNA fragments that accumulate in a PNPase deficient strain showed that Glu tRNA precursors still harbouring the 5' leader can be degraded by a 3' to 5' quality control pathway involving poly(A) polymerase.
Title: Search for poly(A) polymerase targets in E. coli reveals its implication in surveillance of Glu tRNA processing and degradation of stable RNAs.
Null mutations in pcnB, encoding poly(A) polymerase I (PAP I), and in hfq, encoding the RNA chaperone Hfq, suppress toxicity caused by overproduction of SprE.
Title: The response regulator SprE (RssB) modulates polyadenylation and mRNA stability in Escherichia coli.
N-terminus verified by Edman degradation on mature peptide
Title: Identification of the gene for an Escherichia coli poly(A) polymerase.
Data show that the inactivation of Hfq leads to a reduction in the ability of poly(A) polymerase I to add poly(A) tails to mRNAs containing Rho-independent transcription terminators.
Title: The Sm-like protein Hfq regulates polyadenylation dependent mRNA decay in Escherichia coli.
There are three transcription start sites upstream of the pcnB coding sequence; activities of at least two of these promoters are in inverse correlation to bacterial growth rates.
Title: Transcription start sites in the promoter region of the Escherichia coli pcnB (plasmid copy number) gene coding for poly(A) polymerase I.
linker between transcription and mRNA degradation via sigmaS proteolysis with the ultimate goal of adapting and surviving to growth-limiting conditions.
Title: Poly(A)-polymerase I links transcription with mRNA degradation via sigmaS proteolysis.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Gene Ontology Provided by EcoCyc

Function	Evidence Code	Pubs
contributes_to 3'-5' RNA polymerase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables RNA binding	IEA Inferred from Electronic Annotation more info
enables RNA binding	IMP Inferred from Mutant Phenotype more info	PubMed
enables poly(A) RNA polymerase activity	IEA Inferred from Electronic Annotation more info
enables transferase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within RNA modification	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within RNA modification	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within RNA modification	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in RNA processing	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within mRNA processing	IDA Inferred from Direct Assay more info	PubMed
involved_in mRNA processing	IEA Inferred from Electronic Annotation more info
involved_in plasmid maintenance	IEA Inferred from Electronic Annotation more info
involved_in plasmid maintenance	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in polyadenylation-dependent RNA catabolic process	IEA Inferred from Electronic Annotation more info
involved_in polyadenylation-dependent mRNA catabolic process	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytosol	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	IEA Inferred from Electronic Annotation more info