U.S. flag

An official website of the United States government

Format

Send to:

Choose Destination

Links from GEO Profiles

    • Showing Current items.

    drh-3 Helicase ATP-binding domain-containing protein [ Caenorhabditis elegans ]

    Gene ID: 172546, updated on 9-Dec-2024

    Summary

    Go to the top of the page Help
    Official Symbol
    drh-3
    Official Full Name
    Helicase ATP-binding domain-containing protein
    Primary source
    WormBase:WBGene00008400
    Locus tag
    CELE_D2005.5
    See related
    AllianceGenome:WB:WBGene00008400
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Caenorhabditis elegans (strain: Bristol N2)
    Lineage
    Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida; Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae; Caenorhabditis
    Summary
    Enables several functions, including ATP hydrolysis activity; ATP-dependent activity, acting on RNA; and RNA binding activity. Involved in P granule assembly; regulatory ncRNA-mediated post-transcriptional gene silencing; and siRNA processing. Located in P granule; condensed nuclear chromosome; and metaphase plate. Is expressed widely. Orthologous to several human genes including RIGI (RNA sensor RIG-I). [provided by Alliance of Genome Resources, Dec 2024]
    NEW
    Try the new Gene table
    Try the new Transcript table

    Genomic context

    Go to the top of the page Help
    See drh-3 in Genome Data Viewer
    Location:
    chromosome: I
    Exon count:
    15
    Sequence:
    Chromosome: I; NC_003279.8 (7820831..7826378)

    Chromosome I - NC_003279.8Genomic Context describing neighboring genes Neighboring gene Uncharacterized protein Neighboring gene ncRNA Neighboring gene TPR_REGION domain-containing protein Neighboring gene NAM-associated domain-containing protein Neighboring gene Putative glutaminase 3 Neighboring gene RING-type domain-containing protein

    Genomic regions, transcripts, and products

    Go to the top of the page Help
    Genomic Sequence:
    NC_003279.8

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

    Go to the top of the pageHelp

    Related articles in PubMed

    1. Insights into the structure and RNA-binding specificity of Caenorhabditis elegans Dicer-related helicase 3 (DRH-3). Li K, et al. Nucleic Acids Res, 2021 Sep 27. PMID 34403472, Free PMC Article
    2. Dicer-related helicase 3 forms an obligate dimer for recognizing 22G-RNA. Fitzgerald ME, et al. Nucleic Acids Res, 2014 Apr. PMID 24435798, Free PMC Article
    3. Double-stranded RNA-dependent ATPase DRH-3: insight into its role in RNAsilencing in Caenorhabditis elegans. Matranga C, et al. J Biol Chem, 2010 Aug 13. PMID 20529861, Free PMC Article
    4. Homologous RIG-I-like helicase proteins direct RNAi-mediated antiviral immunity in C. elegans by distinct mechanisms. Guo X, et al. Proc Natl Acad Sci U S A, 2013 Oct 1. PMID 24043766, Free PMC Article
    5. A genome-wide survey and systematic RNAi-based characterization of helicase-like genes in Caenorhabditis elegans. Eki T, et al. DNA Res, 2007 Aug 31. PMID 17921522, Free PMC Article

    See all (43) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Purification and Characterization of Double-Stranded Nucleic Acid-Dependent ATPase Activities of Tagged Dicer-Related Helicase 1 and its Short Isoform in Caenorhabditis elegans.
      Title: Purification and Characterization of Double-Stranded Nucleic Acid-Dependent ATPase Activities of Tagged Dicer-Related Helicase 1 and its Short Isoform in Caenorhabditis elegans.
    2. Results show that DRH-3 specifies 22 bp RNAs by dimerization of the helicase domain, a process mediated by ATPase activity and the N-terminal domain.
      Title: Dicer-related helicase 3 forms an obligate dimer for recognizing 22G-RNA.
    3. DRH-3 is required for the biogenesis of virus-derived secondary, but not primary, siRNAs.
      Title: Homologous RIG-I-like helicase proteins direct RNAi-mediated antiviral immunity in C. elegans by distinct mechanisms.
    4. ATPase activity of DRH-3 is stimulated only by double-stranded RNA. DRH-3 is a robust RNA-stimulated ATPase with a k(cat) value of 500/min when stimulated with short RNA duplexes.
      Title: Double-stranded RNA-dependent ATPase DRH-3: insight into its role in RNAsilencing in Caenorhabditis elegans.
    5. the drh-3 gene is essential for the development of germ-lines by maintaining chromosomal integrity in C. elegans.
      Title: Dicer-related drh-3 gene functions in germ-line development by maintenance of chromosomal integrity in Caenorhabditis elegans.
    Submit: New GeneRIF Correction

    Interactions

    Go to the top of the page Help
    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

    Go to the top of the page Help

    Markers

    Homology

    Gene Ontology Provided by WormBase

    Function Evidence Code Pubs
    enables ATP binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables ATP hydrolysis activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables ATP-dependent activity, acting on RNA IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    NOT enables RNA helicase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables RNA polymerase binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables double-stranded RNA binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables helicase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables nucleic acid binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables single-stranded RNA binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in P granule assembly IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in innate immune response IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in regulatory ncRNA-mediated post-transcriptional gene silencing IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in response to stimulus IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in siRNA processing IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in P granule IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in condensed nuclear chromosome IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    is_active_in cytoplasm IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in cytoplasm IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in metaphase plate IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

    Go to the top of the page Help
    Preferred Names
    Helicase ATP-binding domain-containing protein
    NP_492161.3
    • Confirmed by transcript evidence

    NCBI Reference Sequences (RefSeq)

    Go to the top of the page Help

    NEW Try the new Transcript table

    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_003279.8 Reference assembly

      Range
      7820831..7826378
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_059760.7 → NP_492161.3  Helicase ATP-binding domain-containing protein [Caenorhabditis elegans]

      See identical proteins and their annotated locations for NP_492161.3

      Status: REVIEWED

      UniProtKB/TrEMBL
      Q93413
      Conserved Domains (5) summary
      smart00487
      Location:371 → 572
      DEXDc; DEAD-like helicases superfamily
      COG0513
      Location:729 → 993
      SrmB; Superfamily II DNA and RNA helicase [Replication, recombination and repair]
      cd15804
      Location:948 → 1062
      RLR_C; C-terminal domain of Retinoic acid-inducible gene (RIG)-I-like Receptors
      pfam00270
      Location:372 → 545
      DEAD; DEAD/DEAH box helicase
      pfam00271
      Location:749 → 872
      Helicase_C; Helicase conserved C-terminal domain
    Nucleotide Protein
    Heading Accession and Version

    Gene LinkOut

    The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.