U.S. flag

An official website of the United States government

Format

Send to:

Choose Destination

Links from GEO Profiles

    • Showing Current items.

    cbpA curved DNA-binding protein [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 947572, updated on 3-Dec-2024

    Summary

    Go to the top of the page Help
    Official Symbol
    cbpA
    Official Full Name
    curved DNA-binding protein
    Primary source
    ECOCYC:EG12193
    Locus tag
    b1000
    See related
    ASAP:ABE-0003379
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK0991
    Summary
    DnaJ homolog. [More information is available at EcoGene: EG12193]. CbpA has similarity to DnaJ and functions as a co-chaperone with DnaK in vitro . [More information is available at EcoCyc: EG12193].
    NEW
    Try the new Gene table
    Try the new Transcript table

    Genomic context

    Go to the top of the page Help
    Sequence:
    NC_000913.3 (1062855..1063775, complement)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene trimethylamine N-oxide reductase 1 Neighboring gene trimethylamine-N-oxide reductase-specific chaperone Neighboring gene chaperone modulator CbpM Neighboring gene uncharacterized protein YccE Neighboring gene glucose-1-phosphatase

    Genomic regions, transcripts, and products

    Go to the top of the page Help
    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

    Go to the top of the pageHelp

    Related articles in PubMed

    1. A study of the double mutation of dnaJ and cbpA, whose gene products function as molecular chaperones in Escherichia coli. Ueguchi C, et al. J Bacteriol, 1995 Jul. PMID 7601860, Free PMC Article
    2. DNA recognition by Escherichia coli CbpA protein requires a conserved arginine-minor-groove interaction. Chintakayala K, et al. Nucleic Acids Res, 2015 Feb 27. PMID 25670677, Free PMC Article
    3. Dimerization and DNA-dependent aggregation of the Escherichia coli nucleoid protein and chaperone CbpA. Cosgriff S, et al. Mol Microbiol, 2010 Sep. PMID 20633229, Free PMC Article
    4. The cbpA chaperone gene function compensates for dnaJ in lambda plasmid replication during amino acid starvation of Escherichia coli. Wegrzyn A, et al. J Bacteriol, 1996 Oct. PMID 8824642, Free PMC Article
    5. The rpoD gene functions as a multicopy suppressor for mutations in the chaperones, CbpA, DnaJ and DnaK, in Escherichia coli. Shiozawa T, et al. FEMS Microbiol Lett, 1996 May 1. PMID 9026454

    See all (33) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. CbpA recognizes the minor groove of the double helix which is mediated by amino acid side chain R116, which sits at the boundary of the linker and C-terminal domain I.
      Title: DNA recognition by Escherichia coli CbpA protein requires a conserved arginine-minor-groove interaction.
    2. our work identifies a novel role for Fis and incorporates CbpA into the growing network of factors that mediate bacterial chromosome structure
      Title: E. coli Fis protein insulates the cbpA gene from uncontrolled transcription.
    3. Derivatives of CbpA that are unable to dimerize are also unable to bind DNA.
      Title: Dimerization and DNA-dependent aggregation of the Escherichia coli nucleoid protein and chaperone CbpA.
    4. These data demonstrate that CbpA activity is controlled at multiple levels.
      Title: Complex regulation of the DnaJ homolog CbpA by the global regulators sigmaS and Lrp, by the specific inhibitor CbpM, and by the proteolytic degradation of CbpM.
    5. N-terminus verified by Edman degradation on complete protein
      Title: An analogue of the DnaJ molecular chaperone in Escherichia coli.
    6. In a DnaJ mutant both CbpA and DjlA are required for efficient DnaK mediated protein dissaggregation at 42 degrees C.
      Title: The Escherichia coli DjlA and CbpA proteins can substitute for DnaJ in DnaK-mediated protein disaggregation.
    7. We discovered that CbpM specifically inhibits the in vivo activity of CbpA, preventing it from functioning in cell growth and division.
      Title: In vivo modulation of a DnaJ homolog, CbpA, by CbpM.
    Submit: New GeneRIF Correction

    Interactions

    Go to the top of the page Help
    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

    Go to the top of the page Help

    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables DNA binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables DNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables bent DNA binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables bent DNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables identical protein binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables unfolded protein binding IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables unfolded protein binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in chaperone cofactor-dependent protein refolding IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    acts_upstream_of_or_within chaperone cofactor-dependent protein refolding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    acts_upstream_of_or_within chaperone cofactor-dependent protein refolding IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    acts_upstream_of_or_within chaperone cofactor-dependent protein refolding IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in protein folding IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in protein refolding IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    Component Evidence Code Pubs
    located_in bacterial nucleoid IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    is_active_in cytoplasm IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in nucleoid IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

    Go to the top of the page Help
    Preferred Names
    curved DNA-binding protein

    NCBI Reference Sequences (RefSeq)

    Go to the top of the page Help

    NEW Try the new Transcript table

    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      1062855..1063775 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_415520.1 curved DNA-binding protein [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_415520.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A376TJB6
      Conserved Domains (1) summary
      PRK10266
      Location:1 → 306
      PRK10266; curved DNA-binding protein
    Nucleotide Protein
    Heading Accession and Version

    Gene LinkOut

    The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.