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    rluF 23S rRNA pseudouridine(2604) and tRNA(Tyr) pseudouridine(35) synthase [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 948519, updated on 17-Dec-2024

    Summary

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    Official Symbol
    rluF
    Official Full Name
    23S rRNA pseudouridine(2604) and tRNA(Tyr) pseudouridine(35) synthase
    Primary source
    ECOCYC:EG11921
    Locus tag
    b4022
    See related
    ASAP:ABE-0013150
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK4014; yjbC
    Summary
    RluF is the pseudouridine synthase that catalyzes formation of pseudouridine at position 2604 in 23S rRNA. [More information is available at EcoCyc: EG11921].
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    Genomic context

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    Sequence:
    NC_000913.3 (4230354..4231226)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene putative inorganic phosphate export protein YjbB Neighboring gene peptidase E Neighboring gene DUF3811 domain-containing protein YjbD Neighboring gene aspartate kinase III

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Crystal structure of an RluF-RNA complex: a base-pair rearrangement is the key to selectivity of RluF for U2604 of the ribosome. Alian A, et al. J Mol Biol, 2009 May 15. PMID 19298824, Free PMC Article
    2. Domain organization and crystal structure of the catalytic domain of E.coli RluF, a pseudouridine synthase that acts on 23S rRNA. Sunita S, et al. J Mol Biol, 2006 Jun 16. PMID 16712869
    3. Insights into the biology of Escherichia coli through structural proteomics. Matte A, et al. J Struct Funct Genomics, 2007 Sep. PMID 17668295
    4. An indigenous posttranscriptional modification in the ribosomal peptidyl transferase center confers resistance to an array of protein synthesis inhibitors. Toh SM, et al. J Mol Biol, 2008 Jul 18. PMID 18554609, Free PMC Article
    5. Analysis of the Escherichia coli genome. IV. DNA sequence of the region from 89.2 to 92.8 minutes. Blattner FR, et al. Nucleic Acids Res, 1993 Nov 25. PMID 8265357, Free PMC Article

    See all (12) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Analysis of total tRNA isolated from E. coli pseudouridine synthase knock-out mutants identified RluF as the enzyme responsible for this modification.
      Title: Pseudouridine in the Anticodon of Escherichia coli tRNATyr(QΨA) Is Catalyzed by the Dual Specificity Enzyme RluF.
    2. Results describe the crystal structure of an RluF-RNA complex, and suggest that a base-pair rearrangement is the key to selectivity of RluF for U2604 of the ribosome.
      Title: Crystal structure of an RluF-RNA complex: a base-pair rearrangement is the key to selectivity of RluF for U2604 of the ribosome.
    Submit: New GeneRIF Correction

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables 23S rRNA pseudouridine(2604) synthase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables RNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables isomerase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables pseudouridine synthase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables pseudouridine synthase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables pseudouridine synthase activity IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    enables rRNA pseudouridine synthase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables rRNA pseudouridine synthase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables tRNA pseudouridine synthase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in enzyme-directed rRNA pseudouridine synthesis IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within enzyme-directed rRNA pseudouridine synthesis IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in pseudouridine synthesis IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in rRNA processing IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in tRNA processing IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within tRNA pseudouridine synthesis IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    23S rRNA pseudouridine(2604) and tRNA(Tyr) pseudouridine(35) synthase
    NP_418446.1

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      4230354..4231226
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_418446.1 23S rRNA pseudouridine(2604) and tRNA(Tyr) pseudouridine(35) synthase [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_418446.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A444RD14
      Conserved Domains (1) summary
      PRK10475
      Location:1 → 290
      PRK10475; 23S rRNA pseudouridine(2604) synthase RluF
    Nucleotide Protein
    Heading Accession and Version

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