U.S. flag

An official website of the United States government

Format

Send to:

Choose Destination

Links from GEO Profiles

    • Showing Current items.

    hcp protein S-nitrosylase [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 946592, updated on 17-Dec-2024

    Summary

    Go to the top of the page Help
    Official Symbol
    hcp
    Official Full Name
    protein S-nitrosylase
    Primary source
    ECOCYC:G6457
    Locus tag
    b0873
    See related
    ASAP:ABE-0002962
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK0864; priS; ybjW
    Summary
    Hcp is in an operon and associated with the Hcr oxidoreductase. [More information is available at EcoGene: EG13692]. The hybrid cluster protein (HCP) exhibits hydroxylamine reductase activity, possibly functioning as a scavenger of toxic by-products of nitrogen metabolism . [More information is available at EcoCyc: G6457].
    NEW
    Try the new Gene table
    Try the new Transcript table

    Genomic context

    Go to the top of the page Help
    Sequence:
    NC_000913.3 (912162..913814, complement)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene pyruvate oxidase Neighboring gene NADH oxidoreductase Neighboring gene L-lysine/thialysine exporter Neighboring gene water channel AqpZ

    Genomic regions, transcripts, and products

    Go to the top of the page Help
    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

    Go to the top of the pageHelp

    Related articles in PubMed

    1. Three Hcp homologs with divergent extended loop regions exhibit different functions in avian pathogenic Escherichia coli. Ma J, et al. Emerg Microbes Infect, 2018 Mar 29. PMID 29593238, Free PMC Article
    2. Modulation of virulence by two acidified nitrite-responsive loci of Salmonella enterica serovar Typhimurium. Kim CC, et al. Infect Immun, 2003 Jun. PMID 12761099, Free PMC Article
    3. Transcriptional regulation of a hybrid cluster (prismane) protein. Filenko NA, et al. Biochem Soc Trans, 2005 Feb. PMID 15667305
    4. Biochemical and spectroscopic characterization of overexpressed fuscoredoxin from Escherichia coli. Pereira AS, et al. Biochem Biophys Res Commun, 1999 Jun 24. PMID 10381368
    5. Iron-sulfur proteins: new roles for old clusters. Johnson MK. Curr Opin Chem Biol, 1998 Apr. PMID 9667933

    See all (23) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Crystal structure of Escherichia coli class II hybrid cluster protein, HCP, reveals a [4Fe-4S] cluster at the N-terminal protrusion.
      Title: Crystal structure of Escherichia coli class II hybrid cluster protein, HCP, reveals a [4Fe-4S] cluster at the N-terminal protrusion.
    2. Escherichia coli mutants lacking the high-affinity nitric oxide (NO) reductase activity of the hybrid cluster protein (Hcp) are less sensitive to nitrosative stress in the iron-sulfur cluster repair protein (YtfE)(-) background than isogenic ytfE(+) strains. The concerted action of YtfE and Hcp maintains the cytoplasmic NO, and proves the coordinated regulation of Hcp and YtfE synthesis by Transcription Factor NsrR.
      Title: Release of nitric oxide by the Escherichia coli YtfE (RIC) protein and its reduction by the hybrid cluster protein in an integrated pathway to minimize cytoplasmic nitrosative stress.
    3. These results indicated that the functional differentiation of Hcp homologs was driven by differences in transcriptional regulation, extended loop regions, and effector delivery.
      Title: Three Hcp homologs with divergent extended loop regions exhibit different functions in avian pathogenic Escherichia coli.
    4. In an hcr mutant, Hcp is reversibly inactivated by nitric oxide (NO) concentrations above 200 nM, indicating that Hcr protects Hcp from nitrosylation by its substrate, NO.
      Title: The roles of the hybrid cluster protein, Hcp and its reductase, Hcr, in high affinity nitric oxide reduction that protects anaerobic cultures of Escherichia coli against nitrosative stress.
    Submit: New GeneRIF Correction

    Interactions

    Go to the top of the page Help
    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

    Go to the top of the page Help

    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables 2 iron, 2 sulfur cluster binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables hydroxylamine reductase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables hydroxylamine reductase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables hydroxylamine reductase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables iron-sulfur cluster binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables oxidoreductase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables oxidoreductase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables oxidoreductase activity, acting on other nitrogenous compounds as donors IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables peroxidase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables peroxidase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in cellular oxidant detoxification IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in nitric oxide catabolic process IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    acts_upstream_of_or_within nitric oxide catabolic process IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in response to hydrogen peroxide IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    acts_upstream_of_or_within response to hydrogen peroxide IEP
    Inferred from Expression Pattern
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

    Go to the top of the page Help
    Preferred Names
    protein S-nitrosylase
    NP_415394.4

    NCBI Reference Sequences (RefSeq)

    Go to the top of the page Help

    NEW Try the new Transcript table

    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      912162..913814 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_415394.4 protein S-nitrosylase [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_415394.4

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A1X3JK52
      Conserved Domains (1) summary
      PRK05290
      Location:1 → 550
      PRK05290; hybrid cluster protein; Provisional
    Nucleotide Protein
    Heading Accession and Version

    Gene LinkOut

    The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.