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    rnhB RNase HII [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 944852, updated on 3-Dec-2024

    Summary

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    Official Symbol
    rnhB
    Official Full Name
    RNase HII
    Primary source
    ECOCYC:EG10861
    Locus tag
    b0183
    See related
    ASAP:ABE-0000622
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK0182
    Summary
    RNase HII cleaves RNA in RNA-DNA hybrids . [More information is available at EcoCyc: EG10861].
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    Genomic context

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    Sequence:
    NC_000913.3 (204493..205089)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene 3-hydroxy-acyl-[acyl-carrier-protein] dehydratase Neighboring gene acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase Neighboring gene lipid A disaccharide synthase Neighboring gene DNA polymerase III subunit alpha Neighboring gene acetyl-CoA carboxyltransferase subunit alpha

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Mechanisms employed by Escherichia coli to prevent ribonucleotide incorporation into genomic DNA by Pol V. McDonald JP, et al. PLoS Genet, 2012. PMID 23144626, Free PMC Article
    2. The pH-dependence of the Escherichia coli RNase HII-catalysed reaction suggests that an active site carboxylate group participates directly in catalysis. Bastock JA, et al. J Mol Biol, 2007 Apr 27. PMID 17355881
    3. Isolation and characterization of a second RNase H (RNase HII) of Escherichia coli K-12 encoded by the rnhB gene. Itaya M. Proc Natl Acad Sci U S A, 1990 Nov. PMID 2172991, Free PMC Article
    4. RNase HII Saves rnhA Mutant Escherichia coli from R-Loop-Associated Chromosomal Fragmentation. Kouzminova EA, et al. J Mol Biol, 2017 Sep 15. PMID 28821455, Free PMC Article
    5. Ribonuclease H: molecular diversities, substrate binding domains, and catalytic mechanism of the prokaryotic enzymes. Tadokoro T, et al. FEBS J, 2009 Mar. PMID 19228197

    See all (15) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. The authors propose that RNase H-deficient mutants convert some R-loops into R-tracts, which progress into R-gaps and then to double-strand breaks-explaining why R-tracts do not accumulate in RNase H-deficient cells, while double-strand breaks do.
      Title: RNase HII Saves rnhA Mutant Escherichia coli from R-Loop-Associated Chromosomal Fragmentation.
    2. N-terminus verified by Edman degradation on complete protein
      Title: Isolation and characterization of a second RNase H (RNase HII) of Escherichia coli K-12 encoded by the rnhB gene.
    3. The data suggest a requirement for ionisation of an active site carboxylic acid for metal ion binding or correct positioning of metal ion(s) in the enzyme-substrate complex and a role for a second active site carboxylate in general base catalysis.
      Title: The pH-dependence of the Escherichia coli RNase HII-catalysed reaction suggests that an active site carboxylate group participates directly in catalysis.
    Submit: New GeneRIF Correction

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables RNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables RNA-DNA hybrid ribonuclease activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables RNA-DNA hybrid ribonuclease activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables RNA-DNA hybrid ribonuclease activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables endonuclease activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables manganese ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in DNA replication, removal of RNA primer IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in RNA catabolic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in mismatch repair IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    acts_upstream_of_or_within ribonucleotide excision repair EXP
    Inferred from Experiment
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  
    part_of ribonuclease H2 complex IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  

    General protein information

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    Preferred Names
    RNase HII
    NP_414725.1

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      204493..205089
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_414725.1 RNase HII [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_414725.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A831DSJ5, A0A8S7IDK3, A0A8S7XSV8
      Conserved Domains (1) summary
      PRK00015
      Location:10 → 190
      rnhB; ribonuclease HII; Validated
    Nucleotide Protein
    Heading Accession and Version

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