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    obgE GTPase ObgE [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 947694, updated on 3-Dec-2024

    Summary

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    Official Symbol
    obgE
    Official Full Name
    GTPase ObgE
    Primary source
    ECOCYC:G7656
    Locus tag
    b3183
    See related
    ASAP:ABE-0010462
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    cgtA; ECK3172; obg; yhbZ
    Summary
    obgE mutants have lowered DnaA and elevated basal level ppGpp. obgE is required for phage lambda development. [More information is available at EcoGene: EG12795]. ObgE is an essential member of the Obg family of small GTP-binding proteins . [More information is available at EcoCyc: G7656].
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    Genomic context

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    Sequence:
    NC_000913.3 (3330582..3331754, complement)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene transcription elongation factor GreA Neighboring gene peptidoglycan DD-endopeptidase DacB Neighboring gene putative transporter YhbE Neighboring gene 50S ribosomal subunit protein L27

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Involvement of the cgtA gene function in stimulation of DNA repair in Escherichia coli and Vibrio harveyi. Zielke R, et al. Microbiology (Reading), 2003 Jul. PMID 12855728
    2. Obg/CtgA, a signaling protein that controls replication, translation, and morphological development? Michel B. Dev Cell, 2005 Mar. PMID 15737924
    3. A Single-Amino-Acid Substitution in Obg Activates a New Programmed Cell Death Pathway in Escherichia coli. Dewachter L, et al. mBio, 2015 Dec 22. PMID 26695632, Free PMC Article
    4. Structural and functional insights into the mode of action of a universally conserved Obg GTPase. Feng B, et al. PLoS Biol, 2014 May. PMID 24844575, Free PMC Article
    5. Deficiency of essential GTP-binding protein ObgE in Escherichia coli inhibits chromosome partition. Kobayashi G, et al. Mol Microbiol, 2001 Sep. PMID 11555285

    See all (38) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. YbiB: a novel interactor of the GTPase ObgE.
      Title: YbiB: a novel interactor of the GTPase ObgE.
    2. The data suggest that GDP or ppGpp binding is required but not sufficient for ObgE to activate expression of hokB, ultimately leading to persistence.
      Title: Biochemical determinants of ObgE-mediated persistence.
    3. Biophysical analysis, using small angle X-ray scattering and multi-angle light scattering experiments, revealed that ObgE is a monomer in solution, regardless of the bound nucleotide. In contrast to recent suggestions, our biochemical analyses further indicate that ObgE is neither activated by K(+) ions nor by homodimerization.
      Title: Structural and biochemical analysis of Escherichia coli ObgE, a central regulator of bacterial persistence.
    4. obgE is cotranscribed with ribosomal protein genes rplU and rpmA.
      Title: Coexpression of Escherichia coli obgE, Encoding the Evolutionarily Conserved Obg GTPase, with Ribosomal Proteins L21 and L27.
    5. The authors report that expression of a mutant isoform of the essential GTPase ObgE causes rapid loss of viability in Escherichia coli.
      Title: A Single-Amino-Acid Substitution in Obg Activates a New Programmed Cell Death Pathway in Escherichia coli.
    6. ObgE might act as a checkpoint in final stages of the 50S subunit assembly under normal growth conditions, and, as a (p)ppGpp effector, might also have a regulatory role in the production of the 50S subunit under stressed conditions.
      Title: Structural and functional insights into the mode of action of a universally conserved Obg GTPase.
    7. ObgE changes the ratio of ppGpp to pppGpp in the cell during a response to amino acid starvation.
      Title: The ObgE/CgtA GTPase influences the stringent response to amino acid starvation in Escherichia coli.
    8. In this study we apply a new semiquantitative proteomic approach to show that CgtA(E) is required for optimal incorporation of certain late-assembly ribosomal proteins into the large ribosomal subunit.
      Title: The Escherichia coli GTPase CgtAE is involved in late steps of large ribosome assembly.
    9. ObgE is required to license chromosome segregation and subsequent cell cycle events.
      Title: Chromosome segregation control by Escherichia coli ObgE GTPase.
    10. Study proposes that, in addition to its role as a 50S assembly factor, CgtA promotes SpoT (p)ppGpp degradation activity on the ribosome and that the loss of CgtA from the ribosome is necessary for maximal (p)ppGpp accumulation under stress conditions.
      Title: G-protein control of the ribosome-associated stress response protein SpoT.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables DNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables GDP binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables GTP binding IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables GTP binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables GTP binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables GTPase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables GTPase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables GTPase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables guanosine tetraphosphate binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables guanyl ribonucleotide binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables hydrolase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables magnesium ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables rRNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables ribonucleoside triphosphate phosphatase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables ribosomal large subunit binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Process Evidence Code Pubs
    acts_upstream_of_or_within chromosome segregation IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in dormancy process IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in negative regulation of ribosome biogenesis IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in ribosomal large subunit assembly IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in ribosome assembly IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in ribosome biogenesis IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    GTPase ObgE

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      3330582..3331754 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_417650.1 GTPase ObgE [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_417650.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A376M2B5
      Conserved Domains (1) summary
      PRK12298
      Location:1 → 390
      obgE; GTPase CgtA; Reviewed
    Nucleotide Protein
    Heading Accession and Version

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