FLO1 flocculin FLO1 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: FLO1
Official Full Name: flocculin FLO1
Primary source: SGD:S000000084
Locus tag: YAR050W
See related: AllianceGenome:SGD:S000000084; FungiDB:YAR050W; VEuPathDB:YAR050W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: FLO2; FLO4
Summary: Enables D-mannose binding activity. Involved in flocculation. Located in fungal-type cell wall. [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See FLO1 in Genome Data Viewer

Location:: chromosome: I

Exon count:: 1

Sequence:: Chromosome: I; NC_001133.9 (203403..208016)

Chromosome I - NC_001133.9 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001133.9

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Sas3 and Ada2-dependent histone H3 acetylation is required for transcription elongation at the de-repressed FLO1 gene.
Title: Sas3 and Ada2(Gcn5)-dependent histone H3 acetylation is required for transcription elongation at the de-repressed FLO1 gene.
we identified a transposable element insertion in the promoter region of known flocculation gene FLO1, and, in an additional five clones, we recovered loss-of-function mutations in transcriptional repressor TUP1, which regulates FLO1 and other related genes
Title: Experimental Evolution Reveals Favored Adaptive Routes to Cell Aggregation in Yeast.
expression of FLO5 gene was higher than that of FLO1 gene
Title: Genetic diversity of FLO1 and FLO5 genes in wine flocculent Saccharomyces cerevisiae strains.
These data suggest that distinctly bound Cyc8-Tup1 cooperates with Hda1p and Rpd3p to establish or maintain an extensive array of strongly positioned, deacetylated nucleosomes over the FLO1 promoter and upstream region which inhibit histone acetylation, block Swi-Snf binding and prevent transcription.
Title: The yeast Cyc8-Tup1 complex cooperates with Hda1p and Rpd3p histone deacetylases to robustly repress transcription of the subtelomeric FLO1 gene.
Deletion of tandem repeats B, C and D adjacent to the 3'-terminal of FLO1 increases the conformation of flocculin in response to changes of pH and temperature.
Title: [Effect of tandem repeats adjacent to 3'-terminal of FLO1 on the flocculation function of Saccharomyces cerevisiae].
Expression of Lg-FLO1, which is required for flocculation of bottom-fermenting yeast, was also found to be induced by starvation for nitrogen.
Title: Nitrogen starvation induces expression of Lg-FLO1 and flocculation in bottom-fermenting yeast.
Repeated DNA sequence of FLO1 is important for the control of flocculation and genetic stability.
Title: [Regulatory effect of FLO1 tandem repeats on the flocculation characteristics and genetic stability in Saccharomyces cerevisiae].
N-terminal domain of Flo1p contains two mannose carbohydrate binding sites with different affinities
Title: The N-terminal domain of the Flo1 flocculation protein from Saccharomyces cerevisiae binds specifically to mannose carbohydrates.
FLONS, conferring NewFlo-type flocculation ability in yeast was cloned and The 3,396-bp ORF encoded a peptide of 1,132 amino acids with high identity to Flo1 protein
Title: Deletion of tandem repeats causes flocculation phenotype conversion from Flo1 to NewFlo in Saccharomyces cerevisiae.
The authors describe the discovery of an extracellular matrix produced by FLO1-expressing Saccharomyces cerevisiae cells.
Title: Characterization of a biofilm-like extracellular matrix in FLO1-expressing Saccharomyces cerevisiae cells.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables D-mannose binding	IMP Inferred from Mutant Phenotype more info	PubMed

Process	Evidence Code	Pubs
involved_in flocculation	IEA Inferred from Electronic Annotation more info
involved_in flocculation	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in cell periphery	HDA more info	PubMed
located_in extracellular region	IEA Inferred from Electronic Annotation more info
located_in fungal-type cell wall	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	IEA Inferred from Electronic Annotation more info
located_in side of membrane	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: flocculin FLO1

NP_009424.1

Lectin-like protein involved in flocculation; cell wall protein that binds mannose chains on the surface of other cells, confers floc-forming ability that is chymotrypsin sensitive and heat resistant; important for co-flocculation with other yeasts, mediating interaction with specific species; FLO1 has a paralog, FLO5, that arose from a segmental duplication

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001133.9 Reference assembly

Range

203403..208016

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001178230.1 → NP_009424.1 TPA: flocculin FLO1 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_009424.1

Status: REVIEWED

UniProtKB/Swiss-Prot

A7U4Y7, D6VPN7, P32768, Q58HH7, Q58HH8, Q58HH9, Q58HI0

Conserved Domains (2) summary

pfam07691
Location:100 → 249

PA14; PA14 domain

pfam13928
Location:1235 → 1276

Flocculin_t3; Flocculin type 3 repeat