U.S. flag

An official website of the United States government

Format

Send to:

Choose Destination

Links from GEO Profiles

    • Showing Current items.

    POL30 proliferating cell nuclear antigen [ Saccharomyces cerevisiae S288C ]

    Gene ID: 852385, updated on 9-Dec-2024

    Summary

    Go to the top of the page Help
    Official Symbol
    POL30
    Official Full Name
    proliferating cell nuclear antigen
    Primary source
    SGD:S000000292
    Locus tag
    YBR088C
    See related
    AllianceGenome:SGD:S000000292; FungiDB:YBR088C; VEuPathDB:YBR088C
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Saccharomyces cerevisiae S288C (strain: S288C)
    Lineage
    Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
    Summary
    Enables DNA polymerase processivity factor activity. Involved in several processes, including DNA metabolic process; constitutive heterochromatin formation; and establishment of mitotic sister chromatid cohesion. Located in nucleus and replication fork. Part of PCNA complex. Human ortholog(s) of this gene implicated in ataxia-telangiectasia-like disorder-2. Orthologous to human PCNA (proliferating cell nuclear antigen). [provided by Alliance of Genome Resources, Dec 2024]
    NEW
    Try the new Gene table
    Try the new Transcript table

    Genomic context

    Go to the top of the page Help
    See POL30 in Genome Data Viewer
    Location:
    chromosome: II
    Exon count:
    1
    Sequence:
    Chromosome: II; NC_001134.8 (424990..425766, complement)

    Chromosome II - NC_001134.8Genomic Context describing neighboring genes Neighboring gene Ist2p Neighboring gene replication factor C subunit 5 Neighboring gene uncharacterized protein Neighboring gene high-mobility group nucleosome-binding protein Neighboring gene Tim12p

    Genomic regions, transcripts, and products

    Go to the top of the page Help
    Genomic Sequence:
    NC_001134.8

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

    Go to the top of the pageHelp

    Related articles in PubMed

    1. Molecular cloning, structure and expression of the yeast proliferating cell nuclear antigen gene. Bauer GA, et al. Nucleic Acids Res, 1990 Jan 25. PMID 1970160, Free PMC Article
    2. The structure of a ring-opened proliferating cell nuclear antigen-replication factor C complex revealed by fluorescence energy transfer. Zhuang Z, et al. Proc Natl Acad Sci U S A, 2006 Feb 21. PMID 16476998, Free PMC Article
    3. Conformational analysis of processivity clamps in solution demonstrates that tertiary structure does not correlate with protein dynamics. Fang J, et al. Structure, 2014 Apr 8. PMID 24613485, Free PMC Article
    4. Out-of-plane motions in open sliding clamps: molecular dynamics simulations of eukaryotic and archaeal proliferating cell nuclear antigen. Kazmirski SL, et al. Proc Natl Acad Sci U S A, 2005 Sep 27. PMID 16169903, Free PMC Article
    5. DNA-damage tolerance through PCNA ubiquitination and sumoylation. Fan L, et al. Biochem J, 2020 Jul 31. PMID 32726436

    See all (355) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Structural Basis for the Interaction Between Yeast Chromatin Assembly Factor 1 and Proliferating Cell Nuclear Antigen.
      Title: Structural Basis for the Interaction Between Yeast Chromatin Assembly Factor 1 and Proliferating Cell Nuclear Antigen.
    2. Structure of the PCNA unloader Elg1-RFC.
      Title: Structure of the PCNA unloader Elg1-RFC.
    3. Genetic Dissection of Budding Yeast PCNA Mutations Responsible for the Regulated Recruitment of Srs2 Helicase.
      Title: Genetic Dissection of Budding Yeast PCNA Mutations Responsible for the Regulated Recruitment of Srs2 Helicase.
    4. PCNA promotes context-specific sister chromatid cohesion establishment separate from that of chromatin condensation.
      Title: PCNA promotes context-specific sister chromatid cohesion establishment separate from that of chromatin condensation.
    5. Photo-activatable Ub-PCNA probes reveal new structural features of the Saccharomyces cerevisiae Poleta/PCNA complex.
      Title: Photo-activatable Ub-PCNA probes reveal new structural features of the Saccharomyces cerevisiae Polη/PCNA complex.
    6. Limiting DNA polymerase delta alters replication dynamics and leads to a dependence on checkpoint activation and recombination-mediated DNA repair.
      Title: Limiting DNA polymerase delta alters replication dynamics and leads to a dependence on checkpoint activation and recombination-mediated DNA repair.
    7. Access to PCNA by Srs2 and Elg1 Controls the Choice between Alternative Repair Pathways in Saccharomyces cerevisiae.
      Title: Access to PCNA by Srs2 and Elg1 Controls the Choice between Alternative Repair Pathways in Saccharomyces cerevisiae.
    8. DNA-damage tolerance through PCNA ubiquitination and sumoylation.
      Title: DNA-damage tolerance through PCNA ubiquitination and sumoylation.
    9. the silencing-loss phenotypes of pol30-6 and pol30-79 depended on ploidy, but not on POL30 dosage or mating-type identity. Separately from silencing loss, the pol30-6 and pol30-79 alleles also displayed high levels of mitotic recombination in diploids. These results established that histone trafficking involving PCNA at replication forks is crucial to the maintenance of chromatin state and genome stability during DNA repl
      Title: Mutations in the PCNA DNA Polymerase Clamp of Saccharomyces cerevisiae Reveal Complexities of the Cell Cycle and Ploidy on Heterochromatin Assembly.
    10. PCNA retention controlled by the Elg1 complex is critical for efficient MMR: PCNA needs to be on DNA long enough to enable MMR, but if it is retained too long it interferes with downstream repair steps.
      Title: Effective mismatch repair depends on timely control of PCNA retention on DNA by the Elg1 complex.
    Submit: New GeneRIF Correction See all GeneRIFs (50)

    Pathways from PubChem

    Go to the top of the page

    Interactions

    Go to the top of the page Help
    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

    Go to the top of the page Help

    Homology

    Gene Ontology Provided by SGD

    Function Evidence Code Pubs
    enables DNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables DNA polymerase processivity factor activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables DNA polymerase processivity factor activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables DNA polymerase processivity factor activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in DNA repair IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in DNA replication IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in error-free translesion synthesis IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    involved_in establishment of mitotic sister chromatid cohesion IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    involved_in lagging strand elongation IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in lagging strand elongation IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    involved_in leading strand elongation IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in leading strand elongation IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in maintenance of DNA trinucleotide repeats IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    involved_in maintenance of DNA trinucleotide repeats IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in meiotic mismatch repair IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    involved_in meiotic mismatch repair IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in mismatch repair IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in mismatch repair IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    involved_in mismatch repair IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in mismatch repair IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    involved_in mitotic cell cycle IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    involved_in mitotic sister chromatid cohesion IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    involved_in mitotic sister chromatid cohesion IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    involved_in nucleotide-excision repair IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in positive regulation of DNA metabolic process IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in postreplication repair IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    involved_in postreplication repair IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in regulation of DNA replication IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in silent mating-type cassette heterochromatin formation IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    involved_in silent mating-type cassette heterochromatin formation IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in subtelomeric heterochromatin formation IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in translesion synthesis IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    Component Evidence Code Pubs
    part_of PCNA complex IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    part_of PCNA complex IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    located_in chromosome, telomeric region IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in nucleus IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in nucleus IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in replication fork IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

    Go to the top of the page Help
    Preferred Names
    proliferating cell nuclear antigen
    NP_009645.1
    • Proliferating cell nuclear antigen (PCNA); functions as the sliding replication clamp for DNA polymerase delta; may function as docking site for other proteins required for mitotic and meiotic chromosomal DNA replication and for DNA repair; PCNA ubiquitination at K164 plays crucial role during Okazaki fragment processing; Leu164 is essential for stable complex formation with replicative DNA polymerase delta

    NCBI Reference Sequences (RefSeq)

    Go to the top of the page Help

    NEW Try the new Transcript table

    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_001134.8 Reference assembly

      Range
      424990..425766 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_001178436.1 → NP_009645.1  TPA: proliferating cell nuclear antigen [Saccharomyces cerevisiae S288C]

      See identical proteins and their annotated locations for NP_009645.1

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      D6VQ89, P15873
      UniProtKB/TrEMBL
      A0A6B7JGY6, A6ZL36, B3LN62, C7GJS1, D3UEI3, G2W996, N1P843
      Conserved Domains (1) summary
      TIGR00590
      Location:1 → 258
      pcna; proliferating cell nuclear antigen (pcna)
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    P15873.1

    Gene LinkOut

    The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

    Other Literature Sources
    Molecular Biology Databases
    Research Materials
    Tools
    Miscellaneous