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    UBE2F ubiquitin conjugating enzyme E2 F (putative) [ Homo sapiens (human) ]

    Gene ID: 140739, updated on 27-Nov-2024

    Summary

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    Official Symbol
    UBE2Fprovided by HGNC
    Official Full Name
    ubiquitin conjugating enzyme E2 F (putative)provided by HGNC
    Primary source
    HGNC:HGNC:12480
    See related
    Ensembl:ENSG00000184182 MIM:617700; AllianceGenome:HGNC:12480
    Gene type
    protein coding
    RefSeq status
    VALIDATED
    Organism
    Homo sapiens
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
    Also known as
    NCE2
    Summary
    Enables NEDD8 conjugating enzyme activity. Involved in protein neddylation. Predicted to be located in cytosol. Predicted to be active in nucleus. [provided by Alliance of Genome Resources, Nov 2024]
    Expression
    Ubiquitous expression in bone marrow (RPKM 11.8), thyroid (RPKM 10.6) and 25 other tissues See more
    Orthologs
    mouse all
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    Genomic context

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    See UBE2F in Genome Data Viewer
    Location:
    2q37.3
    Exon count:
    12
    Annotation release Status Assembly Chr Location
    RS_2024_08 current GRCh38.p14 (GCF_000001405.40) 2 NC_000002.12 (237967014..238042782)
    RS_2024_08 current T2T-CHM13v2.0 (GCF_009914755.1) 2 NC_060926.1 (238457016..238532785)
    RS_2024_09 previous assembly GRCh37.p13 (GCF_000001405.25) 2 NC_000002.11 (238875656..238951423)

    Chromosome 2 - NC_000002.12Genomic Context describing neighboring genes Neighboring gene uncharacterized LOC105373960 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 12482 Neighboring gene Neanderthal introgressed variant-containing enhancer experimental_57230 Neighboring gene H3K27ac hESC enhancer GRCh37_chr2:238850021-238850520 Neighboring gene uncharacterized LOC124908001 Neighboring gene CRISPRi-validated cis-regulatory element chr2.7218 Neighboring gene CRISPRi-validated cis-regulatory element chr2.7219 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 12483 Neighboring gene UBE2F-SCLY readthrough (NMD candidate) Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr2:238936739-238937352 Neighboring gene ReSE screen-validated silencer GRCh37_chr2:238941390-238941585 Neighboring gene RNA, U6 small nuclear 1333, pseudogene Neighboring gene H3K27ac hESC enhancer GRCh37_chr2:238948423-238948923 Neighboring gene ReSE screen-validated silencer GRCh37_chr2:238951349-238951541 Neighboring gene Neanderthal introgressed variant-containing enhancer experimental_57261 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 12484 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 12485 Neighboring gene selenocysteine lyase Neighboring gene Neanderthal introgressed variant-containing enhancer experimental_57269 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr2:239002815-239003484 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr2:239003485-239004152 Neighboring gene NANOG-H3K27ac-H3K4me1 hESC enhancer GRCh37_chr2:239004821-239005488 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr2:239005489-239006157 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr2:239006158-239006825 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr2:239006826-239007494 Neighboring gene Neanderthal introgressed variant-containing enhancer experimental_57276 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr2:239014380-239015290 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr2:239015291-239016200 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr2:239016201-239017111 Neighboring gene espin like Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 12486 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr2:239039005-239039675

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Expression

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    • Project title: HPA RNA-seq normal tissues
    • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
    • BioProject: PRJEB4337
    • Publication: PMID 24309898
    • Analysis date: Wed Apr 4 07:08:55 2018

    Bibliography

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    Related articles in PubMed

    1. NEDD8-conjugating enzyme E2 UBE2F confers radiation resistance by protecting lung cancer cells from apoptosis. Zhou L, et al. J Zhejiang Univ Sci B, 2021 Nov 15. PMID 34783226, Free PMC Article
    2. UBE2M Is a Stress-Inducible Dual E2 for Neddylation and Ubiquitylation that Promotes Targeted Degradation of UBE2F. Zhou W, et al. Mol Cell, 2018 Jun 21. PMID 29932898, Free PMC Article
    3. A small molecule inhibitor of the UBE2F-CRL5 axis induces apoptosis and radiosensitization in lung cancer. Xu T, et al. Signal Transduct Target Ther, 2022 Oct 17. PMID 36253371, Free PMC Article
    4. Induction of NEDD8-conjugating enzyme E2 UBE2F by platinum protects lung cancer cells from apoptosis and confers to platinum-insensitivity. Zhou L, et al. Cell Death Dis, 2020 Nov 12. PMID 33184273, Free PMC Article
    5. Cullin-5 neddylation-mediated NOXA degradation is enhanced by PRDX1 oligomers in colorectal cancer. Xu S, et al. Cell Death Dis, 2021 Mar 12. PMID 33712558, Free PMC Article

    See all (34) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. NEDD8-conjugating enzyme E2 UBE2F confers radiation resistance by protecting lung cancer cells from apoptosis.
      Title: NEDD8-conjugating enzyme E2 UBE2F confers radiation resistance by protecting lung cancer cells from apoptosis.
    2. Induction of NEDD8-conjugating enzyme E2 UBE2F by platinum protects lung cancer cells from apoptosis and confers to platinum-insensitivity.
      Title: Induction of NEDD8-conjugating enzyme E2 UBE2F by platinum protects lung cancer cells from apoptosis and confers to platinum-insensitivity.
    3. Study establishes a negative regulatory axis between two neddylation E2s with UBE2M ubiquitylating UBE2F, and two CRLs with CRL3 inactivating CRL5.
      Title: UBE2M Is a Stress-Inducible Dual E2 for Neddylation and Ubiquitylation that Promotes Targeted Degradation of UBE2F.
    4. By ubiquitylating and degrading NOXA through activating CRL5, UBE2F selectively promotes lung cancer cell survival and could, therefore, serve as a novel cancer target
      Title: Neddylation E2 UBE2F Promotes the Survival of Lung Cancer Cells by Activating CRL5 to Degrade NOXA via the K11 Linkage.
    5. Distinct preferences of UBC12 and UBE2F peptides for inhibiting different DCNLs, including the oncogenic DCNL1.
      Title: Structural conservation of distinctive N-terminal acetylation-dependent interactions across a family of mammalian NEDD8 ligation enzymes.
    Submit: New GeneRIF Correction

    Phenotypes

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    Find tests for this gene in the NIH Genetic Testing Registry (GTR)

    Review eQTL and phenotype association data in this region using PheGenI

    Variation

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    See Variation Viewer (GRCh37.p13)

    HIV-1 interactions

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    Protein interactions

    Protein Gene Interaction Pubs
    Vif vif The conjugation of NEDD8 to Cullin-5 by UBE2F is required for HIV-1 Vif-mediated A3G degradation PubMed
    vif The active site proximal loop extension (residues 124-129) in UBE2F is critical for HIV-1 Vif-mediated A3G degradation PubMed
    vif UBE2F and RBX2 are required for activation of the polyubiquitin synthesis activity of Vif/CBF-beta/CUL5, leading to HIV-1 Vif-mediated degradation of A3G in cells PubMed

    Go to the HIV-1, Human Interaction Database

    Pathways from PubChem

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    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Markers

    Homology

    Clone Names

    • MGC18120

    Gene Ontology Provided by GOA

    Function Evidence Code Pubs
    enables ATP binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables NEDD8 conjugating enzyme activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables NEDD8 ligase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables NEDD8 transferase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables NEDD8 transferase activity TAS
    Traceable Author Statement
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in post-translational protein modification TAS
    Traceable Author Statement
    more info
    		  
    involved_in protein neddylation IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in protein neddylation IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in protein neddylation IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    is_active_in cytosol IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in cytosol TAS
    Traceable Author Statement
    more info
    		  
    is_active_in nucleus IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  

    General protein information

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    Preferred Names
    NEDD8-conjugating enzyme UBE2F
    Names
    NEDD8 carrier protein UBE2F
    NEDD8 protein ligase UBE2F
    NEDD8-conjugating enzyme 2
    RING-type E3 NEDD8 transferase UBE2F
    ubiquitin-conjugating enzyme E2F (putative)
    NP_001265234.1
    NP_001265235.1
    NP_001265236.1
    NP_001265237.1
    NP_542409.1

    NCBI Reference Sequences (RefSeq)

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    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_001278305.2 → NP_001265234.1  NEDD8-conjugating enzyme UBE2F isoform 1

      See identical proteins and their annotated locations for NP_001265234.1

      Status: VALIDATED

      Description
      Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1. Both variants 1 and 2 encode the same isoform (1).
      Source sequence(s)
      AC016776, AK294107, CN370973, CN370974, DA140546, DB118122
      Consensus CDS
      CCDS2523.1
      UniProtKB/Swiss-Prot
      A8K1Z8, B4DDT9, B4DFI1, B4DMK3, B4DZU2, B8ZZG2, C9J212, H9KVB9, Q969M7
      UniProtKB/TrEMBL
      C9J9P8
      Related
      ENSP00000478474.1, ENST00000612130.4
      Conserved Domains (1) summary
      cl00154
      Location:30 → 184
      UBCc; Ubiquitin-conjugating enzyme E2, catalytic (UBCc) domain. This is part of the ubiquitin-mediated protein degradation pathway in which a thiol-ester linkage forms between a conserved cysteine and the C-terminus of ubiquitin and complexes with ubiquitin ...

    2. NM_001278306.2 → NP_001265235.1  NEDD8-conjugating enzyme UBE2F isoform 2

      See identical proteins and their annotated locations for NP_001265235.1

      Status: VALIDATED

      Description
      Transcript Variant: This variant (3) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at an alternate upstream start codon, compared to variant 1. The encoded isoform (2) is shorter than isoform 1 and has a distinct N-terminus.
      Source sequence(s)
      AK294107, BM470609, BX421077, CN370973, CN370974
      Consensus CDS
      CCDS63175.1
      UniProtKB/Swiss-Prot
      Q969M7
      Related
      ENSP00000386680.1, ENST00000409953.5
      Conserved Domains (1) summary
      COG5078
      Location:16 → 160
      COG5078; Ubiquitin-protein ligase [Posttranslational modification, protein turnover, chaperones]

    3. NM_001278307.2 → NP_001265236.1  NEDD8-conjugating enzyme UBE2F isoform 3

      See identical proteins and their annotated locations for NP_001265236.1

      Status: VALIDATED

      Description
      Transcript Variant: This variant (4) lacks two alternate exons in the 5' coding region, but maintains the reading frame, compared to variant 1. The encoded isoform (3) is shorter than isoform 1.
      Source sequence(s)
      AK297502, CN370973, CN370974
      Consensus CDS
      CCDS63177.1
      UniProtKB/TrEMBL
      C9JF62
      Related
      ENSP00000399183.1, ENST00000414443.5
      Conserved Domains (1) summary
      cl00154
      Location:40 → 152
      UBCc; Ubiquitin-conjugating enzyme E2, catalytic (UBCc) domain. This is part of the ubiquitin-mediated protein degradation pathway in which a thiol-ester linkage forms between a conserved cysteine and the C-terminus of ubiquitin and complexes with ubiquitin ...

    4. NM_001278308.2 → NP_001265237.1  NEDD8-conjugating enzyme UBE2F isoform 4

      See identical proteins and their annotated locations for NP_001265237.1

      Status: VALIDATED

      Description
      Transcript Variant: This variant (5) lacks an alternate exon in the 3' coding region, but maintains the reading frame, compared to variant 1. The encoded isoform (4) is shorter than isoform 1.
      Source sequence(s)
      AK294107, BQ959565, BX421077, CN370973, CN370974
      Consensus CDS
      CCDS63176.1
      UniProtKB/TrEMBL
      C9J9P8
      Related
      ENSP00000387299.1, ENST00000409633.5
      Conserved Domains (1) summary
      cd00195
      Location:35 → 149
      UBCc; Ubiquitin-conjugating enzyme E2, catalytic (UBCc) domain. This is part of the ubiquitin-mediated protein degradation pathway in which a thiol-ester linkage forms between a conserved cysteine and the C-terminus of ubiquitin and complexes with ubiquitin ...

    5. NM_080678.3 → NP_542409.1  NEDD8-conjugating enzyme UBE2F isoform 1

      See identical proteins and their annotated locations for NP_542409.1

      Status: VALIDATED

      Description
      Transcript Variant: This variant (1) encodes the longest isoform (1). Both variants 1 and 2 encode the same isoform (1).
      Source sequence(s)
      AK290063, AK294107, BC010549, BX421077, CN370973
      Consensus CDS
      CCDS2523.1
      UniProtKB/Swiss-Prot
      A8K1Z8, B4DDT9, B4DFI1, B4DMK3, B4DZU2, B8ZZG2, C9J212, H9KVB9, Q969M7
      UniProtKB/TrEMBL
      C9J9P8
      Related
      ENSP00000272930.4, ENST00000272930.9
      Conserved Domains (1) summary
      cl00154
      Location:30 → 184
      UBCc; Ubiquitin-conjugating enzyme E2, catalytic (UBCc) domain. This is part of the ubiquitin-mediated protein degradation pathway in which a thiol-ester linkage forms between a conserved cysteine and the C-terminus of ubiquitin and complexes with ubiquitin ...

    RNA

    1. NR_103498.2 RNA Sequence

      Status: VALIDATED

      Description
      Transcript Variant: This variant (6) lacks an alternate internal exon, compared to variant 1. This variant is represented as non-coding because the use of the 5'-most expected translational start codon, as used in variant 1, renders the transcript a candidate for nonsense-mediated mRNA decay (NMD).
      Source sequence(s)
      AK294107, BX421077, CN370973, CN370974

    2. NR_103499.2 RNA Sequence

      Status: VALIDATED

      Description
      Transcript Variant: This variant (7) lacks two alternate internal exons, compared to variant 1. This variant is represented as non-coding because the use of the 5'-most expected translational start codon, as used in variant 1, renders the transcript a candidate for nonsense-mediated mRNA decay (NMD).
      Source sequence(s)
      AK303094, BQ959565, BX421077, CN370973, CN370974

    3. NR_103500.2 RNA Sequence

      Status: VALIDATED

      Description
      Transcript Variant: This variant (8) contains an alternate internal exon, compared to variant 1. This variant is represented as non-coding because the use of the 5'-most expected translational start codon, as used in variant 1, renders the transcript a candidate for nonsense-mediated mRNA decay (NMD).
      Source sequence(s)
      AK294107, BM920749, BX421077, CN370973, CN370974

    RefSeqs of Annotated Genomes: GCF_000001405.40-RS_2024_08

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCh38.p14 Primary Assembly

    Genomic

    1. NC_000002.12 Reference GRCh38.p14 Primary Assembly

      Range
      237967014..238042782
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    Alternate T2T-CHM13v2.0

    Genomic

    1. NC_060926.1 Alternate T2T-CHM13v2.0

      Range
      238457016..238532785
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q969M7.1 GenPept UniProtKB/Swiss-Prot:Q969M7

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