Rassf1 Ras association (RalGDS/AF-6) domain family member 1 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Rassf1provided by MGI
Official Full Name: Ras association (RalGDS/AF-6) domain family member 1provided by MGI
Primary source: MGI:MGI:1928386
See related: Ensembl:ENSMUSG00000010067 AllianceGenome:MGI:1928386
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: 123F2; RDA32; NORE2A; REH3P21; Rassf1A; Rassf1B; Rassf1C
Summary: Enables small GTPase binding activity. Predicted to be involved in several processes, including Ras protein signal transduction; positive regulation of protein ubiquitination; and protein stabilization. Predicted to act upstream of with a negative effect on regulation of cell cycle G1/S phase transition. Predicted to be located in cytoplasm and microtubule cytoskeleton. Predicted to be active in microtubule cytoskeleton and nucleus. Is expressed in liver and metanephros. Human ortholog(s) of this gene implicated in breast cancer; carcinoma (multiple); reproductive organ cancer (multiple); and urinary bladder cancer. Orthologous to human RASSF1 (Ras association domain family member 1). [provided by Alliance of Genome Resources, Jan 2025]
Expression: Ubiquitous expression in lung adult (RPKM 22.7), testis adult (RPKM 20.7) and 28 other tissues See more
Orthologs: human all
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Genomic context

Location:: 9 F1; 9 58.12 cM

Exon count:: 7

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	9	NC_000075.7 (107428752..107439460)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	9	NC_000075.6 (107551434..107562267)

Chromosome 9 - NC_000075.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

RASSF1A promotes ATM signaling and RASSF1A methylation is a synthetic lethal marker for ATR inhibitors.
Title: RASSF1A promotes ATM signaling and RASSF1A methylation is a synthetic lethal marker for ATR inhibitors.
WDR3 promotes stem cell-like properties in prostate cancer by inhibiting USF2-mediated transcription of RASSF1A.
Title: WDR3 promotes stem cell-like properties in prostate cancer by inhibiting USF2-mediated transcription of RASSF1A.
RASSF1A independence and early galectin-1 upregulation in PIK3CA-induced hepatocarcinogenesis: new therapeutic venues.
Title: RASSF1A independence and early galectin-1 upregulation in PIK3CA-induced hepatocarcinogenesis: new therapeutic venues.
Novel Circulating Hypermethylated RASSF1A ddPCR for Liquid Biopsies in Patients With Pediatric Solid Tumors.
Title: Novel Circulating Hypermethylated RASSF1A ddPCR for Liquid Biopsies in Patients With Pediatric Solid Tumors.
The RASSF1A negatively regulates NF-kappaB and thereby attenuates inflammatory cytokine expression.
Title: The tumor suppressor RASSF1A modulates inflammation and injury in the reperfused murine myocardium.
Findings indicate that compared with the alcoholic steatohepatitis (ASH) group of patients, the expression levels of all three tumor suppressor genes (TSGs) proteins, RUNX family transcription factor 3 (RUNX3), glutathione S-transferase pi 1 (GSTP1), and RASSF1A tumor suppressor (RASSF1), were significantly lower in the non-alcoholic steatohepatitis (NASH) group of patients.
Title: The different expression of tumor suppressors, RASSF1A, RUNX3, and GSTP1, in patients with alcoholic steatohepatitis (ASH) vs non-alcoholic steatohepatitis (NASH).
The authors show that an antisense lncRNA of RASSF1A inhibits the translation of RASSF1A to exacerbate cardiac fibrosis in mice, indicating a potential application of RASSF1-AS1 as a therapy target for cardiac fibrosis.
Title: RASSF1-AS1, an antisense lncRNA of RASSF1A, inhibits the translation of RASSF1A to exacerbate cardiac fibrosis in mice.
Study show that hypoxia promotes stabilization of RASSF1A through NOX-1- and protein kinase C- dependent phosphorylation. HIF-1alpha activates RASSF1A transcription via HIF-binding sites in the RASSF1A promoter region. RASSF1A binds to HIF-1alpha, blocks its prolyl-hydroxylation and proteasomal degradation. Study concludes that RASSF1A-HIF-1alpha forms a feedforward loop driving hypoxia signaling in pulmonary hypertens...
Title: A RASSF1A-HIF1α loop drives Warburg effect in cancer and pulmonary hypertension.
The authors found that haploinsufficiency of Rassf1a was sufficient to dramatically enhance the tumorigenicity of K-Ras in the system.
Title: RASSF1A Deficiency Enhances RAS-Driven Lung Tumorigenesis.
RASSF1A is expressed in differentiating stem cells.RASSF1A promotes YAP and p73 binding to differentiation related genes.RASSF1A uncouples Wnt from Hippo signalling and promotes YAP mediated differentiation via p73.
Title: RASSF1A uncouples Wnt from Hippo signalling and promotes YAP mediated differentiation via p73.

Submit: New GeneRIF Correction See all GeneRIFs (22)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (4)
Targeted (3) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

RH124504 (e-PCR)
- UniSTS:161326

AU044980 (e-PCR)
- UniSTS:159918

Rassf1 (e-PCR), detects polymorphism
- UniSTS:526017

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables identical protein binding	ISO Inferred from Sequence Orthology more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables small GTPase binding	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in DNA damage response	ISO Inferred from Sequence Orthology more info
involved_in DNA damage response	ISS Inferred from Sequence or Structural Similarity more info
involved_in Ras protein signal transduction	IBA Inferred from Biological aspect of Ancestor more info
involved_in Ras protein signal transduction	ISO Inferred from Sequence Orthology more info
involved_in Ras protein signal transduction	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of protein ubiquitination	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of protein ubiquitination	ISS Inferred from Sequence or Structural Similarity more info
involved_in protein stabilization	ISO Inferred from Sequence Orthology more info
involved_in protein stabilization	ISS Inferred from Sequence or Structural Similarity more info
involved_in regulation of cell cycle	ISO Inferred from Sequence Orthology more info
involved_in regulation of cell cycle	ISS Inferred from Sequence or Structural Similarity more info
acts_upstream_of_negative_effect regulation of cell cycle G1/S phase transition	ISO Inferred from Sequence Orthology more info
acts_upstream_of_negative_effect regulation of cell cycle G1/S phase transition	ISS Inferred from Sequence or Structural Similarity more info

Component	Evidence Code	Pubs
located_in centrosome	IEA Inferred from Electronic Annotation more info
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in microtubule	IEA Inferred from Electronic Annotation more info
is_active_in microtubule cytoskeleton	IBA Inferred from Biological aspect of Ancestor more info
located_in microtubule cytoskeleton	ISO Inferred from Sequence Orthology more info
located_in microtubule cytoskeleton	ISS Inferred from Sequence or Structural Similarity more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	ISO Inferred from Sequence Orthology more info
located_in nucleus	ISS Inferred from Sequence or Structural Similarity more info
located_in spindle pole	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: ras association domain-containing protein 1

Names: 123F protein; RASSF1A tumor suppressor; Ras association (RalGDS/AF-6) domain family 1; protein 123F2

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001243748.2 → NP_001230677.1 ras association domain-containing protein 1 isoform 1

Status: VALIDATED

Source sequence(s)

AL672219

Consensus CDS

CCDS57699.1

UniProtKB/Swiss-Prot

Q99MK9, Q9WUF5

UniProtKB/TrEMBL

A9YZW8, B0QZV7

Related

ENSMUSP00000091301.3, ENSMUST00000093786.9

Conserved Domains (3) summary

cd00029
Location:52 → 101

C1; Protein kinase C conserved region 1 (C1) . Cysteine-rich zinc binding domain. Some members of this domain family bind phorbol esters and diacylglycerol, some are reported to bind RasGTP. May occur in tandem arrangement. Diacylglycerol (DAG) is a second ...

cd17218
Location:130 → 286

RA_RASSF1; Ras-associating (RA) domain found in Ras-association domain-containing protein 1 (RASSF1)

pfam16517
Location:295 → 334

Nore1-SARAH; Novel Ras effector 1 C-terminal SARAH (Sav/Rassf/Hpo) domain
NM_001411798.1 → NP_001398727.1 ras association domain-containing protein 1 isoform 3

Status: VALIDATED

Source sequence(s)

AL672219

UniProtKB/TrEMBL

Q3UIV8
NM_001411799.1 → NP_001398728.1 ras association domain-containing protein 1 isoform 4

Status: VALIDATED

Source sequence(s)

AL672219
NM_001411800.1 → NP_001398729.1 ras association domain-containing protein 1 isoform 4

Status: VALIDATED

Source sequence(s)

AL672219
NM_001411801.1 → NP_001398730.1 ras association domain-containing protein 1 isoform 4

Status: VALIDATED

Source sequence(s)

AL672219
NM_019713.5 → NP_062687.1 ras association domain-containing protein 1 isoform 2

Status: VALIDATED

Source sequence(s)

AL672219

Consensus CDS

CCDS23494.1

UniProtKB/Swiss-Prot

Q99MK9

UniProtKB/TrEMBL

Q3UIV8

Related

ENSMUSP00000010211.5, ENSMUST00000010211.7

Conserved Domains (2) summary

cd17218
Location:60 → 216

RA_RASSF1; Ras-associating (RA) domain found in Ras-association domain-containing protein 1 (RASSF1)

pfam16517
Location:225 → 264

Nore1-SARAH; Novel Ras effector 1 C-terminal SARAH (Sav/Rassf/Hpo) domain