RDH54 DNA-dependent ATPase RDH54 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: RDH54
Official Full Name: DNA-dependent ATPase RDH54
Primary source: SGD:S000000277
Locus tag: YBR073W
See related: AllianceGenome:SGD:S000000277; FungiDB:YBR073W; VEuPathDB:YBR073W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: TID1
Summary: Enables DNA translocase activity and double-stranded DNA binding activity. Involved in DNA metabolic process and chromosome organization. Predicted to be active in nucleus. Human ortholog(s) of this gene implicated in colorectal cancer and non-Hodgkin lymphoma. Orthologous to human RAD54B (RAD54 homolog B). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See RDH54 in Genome Data Viewer

Location:: chromosome: II

Exon count:: 1

Sequence:: Chromosome: II; NC_001134.8 (383112..385988)

Chromosome II - NC_001134.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001134.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Rad53 regulates the lifetime of Rdh54 at homologous recombination intermediates.
Title: Rad53 regulates the lifetime of Rdh54 at homologous recombination intermediates.
Rad54 and Rdh54 prevent Srs2-mediated disruption of Rad51 presynaptic filaments.
Title: Rad54 and Rdh54 prevent Srs2-mediated disruption of Rad51 presynaptic filaments.
Rad54 and Rdh54 occupy spatially and functionally distinct sites within the Rad51-ssDNA presynaptic complex.
Title: Rad54 and Rdh54 occupy spatially and functionally distinct sites within the Rad51-ssDNA presynaptic complex.
Rdh54, a chromatin remodeler was identified as the first protein required for template switching that does not affect simple gene conversion.
Title: Frequent Interchromosomal Template Switches during Gene Conversion in S. cerevisiae.
Data indicate that the Rad51-binding domain of Rdh54 resides within its N terminus and is required for Rad51 removal from DNA.
Title: Characterization of the interaction between the Saccharomyces cerevisiae Rad51 recombinase and the DNA translocase Rdh54.
Tid1 is a novel target of the DNA damage checkpoint pathway.
Title: Tid1/Rdh54 translocase is phosphorylated through a Mec1- and Rad53-dependent manner in the presence of DSB lesions in budding yeast.
Rad51-Rad54 function together to promote intersister DNA strand exchange, whereas Dmc1-Tid1 tilt the bias toward interhomolog DNA strand exchange.
Title: Saccharomyces cerevisiae Dmc1 and Rad51 proteins preferentially function with Tid1 and Rad54 proteins, respectively, to promote DNA strand invasion during genetic recombination.
Rad54 and Rdh54 have different in vivo interactions with Rad51.
Title: Analyses of the yeast Rad51 recombinase A265V mutant reveal different in vivo roles of Swi2-like factors.
In the absence of the Rad54, Rdh54, and Uls1 translocases, Rad51 accumulates spontaneously on chromatin, blocking cell growth and increasing genomic instability. The translocases dissociate Rad51 from sites of nonspecific dsDNA binding during mitosis.
Title: Swi2/Snf2-related translocases prevent accumulation of toxic Rad51 complexes during mitotic growth.
Rdh54p presumably operates via chromatin remodulation at the site of damage rendering DNA accessible to the DNA repair enzymes.
Title: [The Rdh54 protein role in regulation of DNA repair in yeast Saccharomyces cerevisiae].

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables ATP hydrolysis activity	IEA Inferred from Electronic Annotation more info
enables ATP-dependent H2AZ histone chaperone activity	IEA Inferred from Electronic Annotation more info
enables ATP-dependent H3-H4 histone complex chaperone activity	IEA Inferred from Electronic Annotation more info
enables ATP-dependent activity, acting on DNA	IDA Inferred from Direct Assay more info	PubMed
enables ATP-dependent activity, acting on DNA	IMP Inferred from Mutant Phenotype more info	PubMed
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables DNA clamp loader activity	IEA Inferred from Electronic Annotation more info
enables DNA translocase activity	IBA Inferred from Biological aspect of Ancestor more info
enables DNA translocase activity	IDA Inferred from Direct Assay more info	PubMed
enables chromatin extrusion motor activity	IEA Inferred from Electronic Annotation more info
enables cohesin loader activity	IEA Inferred from Electronic Annotation more info
enables double-stranded DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables double-stranded DNA binding	IMP Inferred from Mutant Phenotype more info	PubMed
enables double-stranded DNA helicase activity	IEA Inferred from Electronic Annotation more info
enables forked DNA-dependent helicase activity	IEA Inferred from Electronic Annotation more info
enables four-way junction helicase activity	IEA Inferred from Electronic Annotation more info
enables helicase activity	IEA Inferred from Electronic Annotation more info
enables hydrolase activity	IEA Inferred from Electronic Annotation more info
enables single-stranded 3'-5' DNA helicase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in DNA duplex unwinding	IDA Inferred from Direct Assay more info	PubMed
involved_in DNA duplex unwinding	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in DNA geometric change	IDA Inferred from Direct Assay more info	PubMed
involved_in DNA geometric change	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in DNA recombination	IEA Inferred from Electronic Annotation more info
involved_in DNA recombination	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in DNA repair	IEA Inferred from Electronic Annotation more info
involved_in DNA repair	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in chromatin looping	IEA Inferred from Electronic Annotation more info
involved_in chromatin remodeling	IEA Inferred from Electronic Annotation more info
involved_in double-strand break repair via homologous recombination	IBA Inferred from Biological aspect of Ancestor more info
involved_in heteroduplex formation	IDA Inferred from Direct Assay more info	PubMed
involved_in meiotic cell cycle	IEA Inferred from Electronic Annotation more info
involved_in meiotic sister chromatid segregation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in reciprocal meiotic recombination	IBA Inferred from Biological aspect of Ancestor more info
involved_in reciprocal meiotic recombination	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IC Inferred by Curator more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: DNA-dependent ATPase RDH54

NP_009629.6

EC 3.6.4.12

DNA-dependent ATPase; DNA recombination/repair translocase, supercoils DNA, promotes DNA strand opening; stimulates strand exchange by modifying dsDNA topology; involved in recombinational repair of DNA double-strand breaks (DSBs) during mitosis, meiosis; implicated in cell divisions in telomerase-negative strains through adaptation to DNA damage, which allows bypass of cell cycle arrest and contributes senescence-specific genome instability; phosphorylated in response to DNA damage

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001134.8 Reference assembly

Range

383112..385988

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001178421.4 → NP_009629.6 TPA: DNA-dependent ATPase RDH54 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_009629.6

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VQ73, P38086

UniProtKB/TrEMBL

G2W979, N1PAS2

Conserved Domains (3) summary

cd18793
Location:663 → 789

SF2_C_SNF; C-terminal helicase domain of the SNF family helicases

cd18004
Location:296 → 573

DEXHc_RAD54; DEXH-box helicase domain of RAD54

pfam10382
Location:108 → 158

DUF2439; Protein of unknown function (DUF2439)