STT3B STT3 oligosaccharyltransferase complex catalytic subunit B [Homo sapiens (human)] - Gene

Summary

Official Symbol: STT3Bprovided by HGNC
Official Full Name: STT3 oligosaccharyltransferase complex catalytic subunit Bprovided by HGNC
Primary source: HGNC:HGNC:30611
See related: Ensembl:ENSG00000163527 MIM:608605; AllianceGenome:HGNC:30611
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Homo sapiens
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as: SIMP; CDG1X; STT3-B
Summary: The protein encoded by this gene is a catalytic subunit of a protein complex that transfers oligosaccharides onto asparagine residues. Defects in this gene are a cause of congenital disorder of glycosylation Ix (CDG1X). [provided by RefSeq, Jun 2014]
Expression: Ubiquitous expression in testis (RPKM 42.8), thyroid (RPKM 37.3) and 25 other tissues See more
Orthologs: mouse all
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Genomic context

Location:: 3p23

Exon count:: 18

Annotation release	Status	Assembly	Chr	Location
RS_2024_08	current	GRCh38.p14 (GCF_000001405.40)	3	NC_000003.12 (31532925..31637616)
RS_2024_08	current	T2T-CHM13v2.0 (GCF_009914755.1)	3	NC_060927.1 (31535391..31640070)
RS_2024_09	previous assembly	GRCh37.p13 (GCF_000001405.25)	3	NC_000003.11 (31574417..31679108)

Chromosome 3 - NC_000003.12 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Expression

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See details

Project title: Tissue-specific circular RNA induction during human fetal development
Description: 35 human fetal samples from 6 tissues (3 - 7 replicates per tissue) collected between 10 and 20 weeks gestational time were sequenced using Illumina TruSeq Stranded Total RNA
BioProject: PRJNA270632
Publication: PMID 26076956
Analysis date: Mon Apr 2 22:54:59 2018

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Stabilization of EREG via STT3B-mediated N-glycosylation is critical for PDL1 upregulation and immune evasion in head and neck squamous cell carcinoma.
Title: Stabilization of EREG via STT3B-mediated N-glycosylation is critical for PDL1 upregulation and immune evasion in head and neck squamous cell carcinoma.
Proteome and Glycoproteome Analyses Reveal the Protein N-Linked Glycosylation Specificity of STT3A and STT3B.
Title: Proteome and Glycoproteome Analyses Reveal the Protein N-Linked Glycosylation Specificity of STT3A and STT3B.
Acceptor sites located in short loops of multi-spanning membrane proteins represent a new class of STT3B-dependent site.
Title: Quantitative glycoproteomics reveals new classes of STT3A- and STT3B-dependent N-glycosylation sites.
The endoplasmic reticulum-localized mEGFP mutants contained an N-glycosylation sequon at their C-terminus and had increased fluorescence upon N-glycosylation. Among the mutants tested, the ER-mEGFP mutant containing the N185 -C186 -T187 sequon was the best substrate for the STT3B isoform in terms of glycosylation efficiency and fluorescence change.
Title: Construction of green fluorescence protein mutant to monitor STT3B-dependent N-glycosylation.
Study reports that STT3B-oligosaccharyltransferase, but not STT3A-oligosaccharyltransferase, is a lipid-linked oligosaccharide hydrolase.
Title: Mammalian STT3A/B oligosaccharyltransferases segregate N-glycosylation at the translocon from lipid-linked oligosaccharide hydrolysis.
These results reveal that the oxidoreductase activity of the STT3B-containing oligosaccharyltransferase is necessary for dengue virus infection.
Title: Dengue Virus Hijacks a Noncanonical Oxidoreductase Function of a Cellular Oligosaccharyltransferase Complex.
STT3B was significantly upregulated in hip osteoarthritis with affected versus intact cartilage, particularly in the analysis of hypertrophic and normotrophic compared with atrophic bone remodelling pattern.
Title: Radiographic endophenotyping in hip osteoarthritis improves the precision of genetic association analysis.
Post-translational modification of cotranslationally skipped sites by STT3B is hindered by the middle X residue, resulting in hypoglycosylation of consensus sites containing large hydrophobic and negatively charged side chains.
Title: The middle X residue influences cotranslational N-glycosylation consensus site skipping.
Results show homozygous mutation in STT3A and in STT3B causes congenital disorders of glycosylation.
Title: Mutations in STT3A and STT3B cause two congenital disorders of glycosylation.
Extreme C-terminal sites are posttranslocationally glycosylated by the STT3B isoform of the OST.
Title: Extreme C-terminal sites are posttranslocationally glycosylated by the STT3B isoform of the OST.

Submit: New GeneRIF Correction See all GeneRIFs (14)

Phenotypes

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Find tests for this gene in the NIH Genetic Testing Registry (GTR)

Review eQTL and phenotype association data in this region using PheGenI

Associated conditions

Description	Tests
STT3B-congenital disorder of glycosylation MedGen: C2931007 OMIM: 615597 GeneReviews: Not available	Compare labs

EBI GWAS Catalog

Description
Genome-wide association study of periodontal health measured by probing depth in adults ages 18-49 years. EBI GWAS Catalog EBI GWAS CatalogPubMed

Variation

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See variants in ClinVar

See studies and variants in dbVar

See Variation Viewer (GRCh37.p13)

See Variation Viewer (GRCh38)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

RH92648 (e-PCR)
- UniSTS:87574

RH103263 (e-PCR)
- UniSTS:97595

D10S16 (e-PCR)
- UniSTS:155756

STS-AA032256 (e-PCR)
- UniSTS:31922

SHGC-80983 (e-PCR)
- UniSTS:102608

RH102149 (e-PCR)
- UniSTS:96483

Gene Ontology Provided by GOA

Function	Evidence Code	Pubs
enables dolichyl-diphosphooligosaccharide-protein glycotransferase activity	IBA Inferred from Biological aspect of Ancestor more info
enables dolichyl-diphosphooligosaccharide-protein glycotransferase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables dolichyl-diphosphooligosaccharide-protein glycotransferase activity	ISS Inferred from Sequence or Structural Similarity more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in ERAD pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in co-translational protein modification	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in glycoprotein catabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in post-translational protein modification	IBA Inferred from Biological aspect of Ancestor more info
involved_in post-translational protein modification	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein N-linked glycosylation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein N-linked glycosylation via asparagine	IBA Inferred from Biological aspect of Ancestor more info
involved_in protein N-linked glycosylation via asparagine	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein N-linked glycosylation via asparagine	ISS Inferred from Sequence or Structural Similarity more info
involved_in response to unfolded protein	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in endoplasmic reticulum	IDA Inferred from Direct Assay more info
located_in endoplasmic reticulum membrane	NAS Non-traceable Author Statement more info	PubMed
located_in membrane	HDA more info	PubMed
part_of oligosaccharyltransferase complex	IDA Inferred from Direct Assay more info	PubMed
part_of oligosaccharyltransferase complex	IPI Inferred from Physical Interaction more info	PubMed
part_of oligosaccharyltransferase complex	ISS Inferred from Sequence or Structural Similarity more info
part_of oligosaccharyltransferase complex	TAS Traceable Author Statement more info	PubMed
part_of protein-containing complex	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3B

Names: STT3, subunit of the oligosaccharyltransferase complex, homolog B; STT3B, catalytic subunit of the oligosaccharyltransferase complex; STT3B, subunit of the oligosaccharyltransferase complex (catalytic); dolichyl-diphosphooligosaccharide protein glycotransferase; homolog of yeast STT3; oligosaccharyl transferase subunit STT3B; source of immunodominant MHC-associated peptides homolog

NP_849193.1

EC 2.4.99.18

XP_011531767.1

EC 2.4.99.18

XP_016861347.1

EC 2.4.99.18

XP_054201614.1

EC 2.4.99.18

XP_054201615.1

EC 2.4.99.18

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

Genomic

NG_034164.1 RefSeqGene

Range

5425..110116

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_178862.3 → NP_849193.1 dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3B

See identical proteins and their annotated locations for NP_849193.1

Status: REVIEWED

Source sequence(s)

AC104643, AI914841, AK075380, AY074880, HY015137, HY039873

Consensus CDS

CCDS2650.1

UniProtKB/Swiss-Prot

Q8TCJ2, Q96JZ4, Q96KY7

Related

ENSP00000295770.2, ENST00000295770.4

Conserved Domains (1) summary

pfam02516
Location:71 → 563

STT3; Oligosaccharyl transferase STT3 subunit

RefSeqs of Annotated Genomes: GCF_000001405.40-RS_2024_08

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p14 Primary Assembly

Genomic

NC_000003.12 Reference GRCh38.p14 Primary Assembly

Range

31532925..31637616

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_011533465.2 → XP_011531767.1 dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3B isoform X2

See identical proteins and their annotated locations for XP_011531767.1

Conserved Domains (1) summary

pfam02516
Location:71 → 442

STT3; Oligosaccharyl transferase STT3 subunit
XM_017005858.2 → XP_016861347.1 dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3B isoform X1

Related

ENSP00000520729.1, ENST00000462235.6

Conserved Domains (1) summary

pfam02516
Location:1 → 417

STT3; Oligosaccharyl transferase STT3 subunit

Alternate T2T-CHM13v2.0

Genomic

NC_060927.1 Alternate T2T-CHM13v2.0

Range

31535391..31640070

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_054345640.1 → XP_054201615.1 dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3B isoform X2
XM_054345639.1 → XP_054201614.1 dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3B isoform X1