not non-stop [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: notprovided by FlyBase
Official Full Name: non-stopprovided by FlyBase
Primary source: FLYBASE:FBgn0013717
Locus tag: Dmel_CG4166
See related: AllianceGenome:FB:FBgn0013717
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: anon-WO0257455.9; CG4166; Dmel\CG4166; dNonstop; dNonStop; i155; l(3)02069; l(3)j9A6; Non-stop; nonstop; Nonstop; Not; NOT; Q9VVR1; UBP8; Upb8; USP22
Summary: Predicted to enable cysteine-type deubiquitinase activity. Involved in several processes, including neurogenesis; positive regulation of DNA-templated transcription; and ubiquitin-dependent protein catabolic process. Located in nucleus. Part of DUBm complex and SAGA complex. Is expressed in CNS glial cell; embryonic/larval optic lobe; lamina anlage; and larval brain. Human ortholog(s) of this gene implicated in non-syndromic X-linked intellectual disability 105. Orthologous to several human genes including USP22 (ubiquitin specific peptidase 22). [provided by Alliance of Genome Resources, Dec 2024]
Orthologs: all
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Genomic context

Location:: 75D4-75D4; 3-45 cM

Exon count:: 5

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	3L	NT_037436.4 (18618305..18622129, complement)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	3L	NT_037436.3 (18611405..18615229, complement)

Chromosome 3L - NT_037436.4 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Drosophila USP22/nonstop polarizes the actin cytoskeleton during collective border cell migration.
Title: Drosophila USP22/nonstop polarizes the actin cytoskeleton during collective border cell migration.
The data indicate that the germline-specific nuclear functions of the Drosophila Ccr4-Not complex involve the repression of especially dangerous active transposable elements as well as the nuclear biogenesis of telomeric transcripts.
Title: Nuclear Ccr4-Not mediates the degradation of telomeric and transposon transcripts at chromatin in the Drosophila germline.
Atxn7 amino terminus anchors Non-stop, a deubiquitinase, to the SAGA chromatin remodeling complex. There, Non-stop countered polyubiquitination and proteasomal degradation of Arp2/3 and WAVE regulatory complex (WRC) subunit SCAR.
Title: Ataxin-7 and Non-stop coordinate SCAR protein levels, subcellular localization, and actin cytoskeleton organization.
Loss of Ataxin-7 results in a toxic gain of activity of the Non-stop deubiquitinase.
Title: Drosophila models reveal novel insights into mechanisms underlying neurodegeneration.
Data establish that Nonstop actually functions as an ubiquitin protease to control the levels of ubiquitinated histone H2B in flies. We further establish that Nonstop is the functional homolog of yeast Ubp8
Title: SAGA-mediated H2B deubiquitination controls the development of neuronal connectivity in the Drosophila visual system.
3 additional subunits of TFTC/STAGA (dSgf11, non-stop, & en(y)2) help form the deubiquitination module. This module is an enhancer of position effect variegation in Drosophila.
Title: A TFTC/STAGA module mediates histone H2A and H2B deubiquitination, coactivates nuclear receptors, and counteracts heterochromatin silencing.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

Dm0634 (e-PCR)
- UniSTS:49664

Homology

NCBI Orthologs

Orthologs from OrthoDB

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables cysteine-type deubiquitinase activity	IEA Inferred from Electronic Annotation more info
enables cysteine-type deubiquitinase activity	ISM Inferred from Sequence Model more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables zinc ion binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in axon target recognition	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in chromatin organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in glial cell development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in glial cell migration	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of DNA-templated transcription	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein deubiquitination	IEA Inferred from Electronic Annotation more info
involved_in regulation of DNA-templated transcription	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in ubiquitin-dependent protein catabolic process	IGI Inferred from Genetic Interaction more info	PubMed

Component	Evidence Code	Pubs
part_of DUBm complex	IDA Inferred from Direct Assay more info	PubMed
part_of SAGA complex	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: non-stop

Names: CG4166-PB; CG4166-PC; CG4166-PD; Non-stop; nonstop; not-PB; not-PC; not-PD

NP_001287106.1

EC 3.4.19.12

NP_001401041.1

EC 3.4.19.12

NP_524140.2

EC 3.4.19.12

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NT_037436.4 Reference assembly

Range

18618305..18622129 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001300177.1 → NP_001287106.1 non-stop, isoform C [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001287106.1

Status: REVIEWED

UniProtKB/Swiss-Prot

Q95TK9, Q9U6Q9, Q9VVR1, X2JCK7

Conserved Domains (2) summary

cd02660
Location:158 → 489

Peptidase_C19D; A subfamily of Peptidase C19. Peptidase C19 contains ubiquitinyl hydrolases. They are intracellular peptidases that remove ubiquitin molecules from polyubiquinated peptides by cleavage of isopeptide bonds. They hydrolyze bonds involving the carboxyl ...

pfam02148
Location:46 → 103

zf-UBP; Zn-finger in ubiquitin-hydrolases and other protein
NM_001414092.1 → NP_001401041.1 non-stop, isoform D [Drosophila melanogaster]

Status: REVIEWED

UniProtKB/TrEMBL

A0A9F2H0Z2
NM_079416.4 → NP_524140.2 non-stop, isoform B [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_524140.2

Status: REVIEWED

UniProtKB/Swiss-Prot

Q95TK9, Q9U6Q9, Q9VVR1, X2JCK7

Conserved Domains (2) summary

cd02660
Location:158 → 489

Peptidase_C19D; A subfamily of Peptidase C19. Peptidase C19 contains ubiquitinyl hydrolases. They are intracellular peptidases that remove ubiquitin molecules from polyubiquinated peptides by cleavage of isopeptide bonds. They hydrolyze bonds involving the carboxyl ...

pfam02148
Location:46 → 103

zf-UBP; Zn-finger in ubiquitin-hydrolases and other protein