ISW2 DNA translocase [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: ISW2
Official Full Name: DNA translocase
Primary source: SGD:S000005831
Locus tag: YOR304W
See related: AllianceGenome:SGD:S000005831; FungiDB:YOR304W; VEuPathDB:YOR304W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables DNA binding activity; DNA translocase activity; and chromatin binding activity. Involved in cellular response to pheromone; negative regulation of macromolecule biosynthetic process; and termination of RNA polymerase II transcription. Located in nuclear microtubule. Part of CHRAC. Orthologous to human SMARCA1 (SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 1) and SMARCA5 (SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 5). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See ISW2 in Genome Data Viewer

Location:: chromosome: XV

Exon count:: 1

Sequence:: Chromosome: XV; NC_001147.6 (884514..887876)

Chromosome XV - NC_001147.6 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001147.6

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Dynamic 1D search and processive nucleosome translocations by RSC and ISW2 chromatin remodelers.
Title: Dynamic 1D search and processive nucleosome translocations by RSC and ISW2 chromatin remodelers.
Basis of specificity for a conserved and promiscuous chromatin remodeling protein.
Title: Basis of specificity for a conserved and promiscuous chromatin remodeling protein.
Histone Octamer Structure Is Altered Early in ISW2 ATP-Dependent Nucleosome Remodeling.
Title: Histone Octamer Structure Is Altered Early in ISW2 ATP-Dependent Nucleosome Remodeling.
we report that Isw2 and Ino80 promote efficient firing of the ribosomal origin of replication and facilitate the regulated increase of rDNA repeat copy number. This work significantly expands our understanding of the importance of ATP-dependent chromatin remodeling for rDNA biology.
Title: Chromatin Remodeling Factors Isw2 and Ino80 Regulate Chromatin, Replication, and Copy Number of the Saccharomyces cerevisiae Ribosomal DNA Locus.
single mutations in genes ISW1, ISW2, and CHD1 weakly affect repair and mutagenic processes in yeast cells.
Title: [The role of remodeling complexes CHD1 and ISWI in spontaneous and UV-induced mutagenesis control in yeast Saccharomyces cerevisiae].
Histone hyperacetylation does not necessarily block ISWI functions and that ISWI enzymes can function on active chromatin as well.
Title: Mobilization of hyperacetylated mononucleosomes by purified yeast ISW2 in vitro.
the ISWI class of ATP-dependent chromatin remodeling complexes plays a conserved role during aging and in calorie restriction.
Title: Inactivation of yeast Isw2 chromatin remodeling enzyme mimics longevity effect of calorie restriction via induction of genotoxic stress response.
Isw2 showed little evidence of nucleosome disassembly.
Title: Comparison of the Isw1a, Isw1b, and Isw2 nucleosome disrupting activities.
Present evidence that a major targeting mechanism for the Isw2 chromatin remodeling enzyme to specific genomic loci is through sequence-specific transcription factor (TF)-dependent recruitment.
Title: DNA looping facilitates targeting of a chromatin remodeling enzyme.
We find that remodelers( Isw1, Isw2, and Chd1 ) occupancy at NDRs and gene bodies is associated with nucleosome turnover and transcriptional elongation rate
Title: ISWI and CHD chromatin remodelers bind promoters but act in gene bodies.

Submit: New GeneRIF Correction See all GeneRIFs (26)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables DNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables DNA translocase activity	IDA Inferred from Direct Assay more info	PubMed
enables centromeric DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables chromatin binding	IBA Inferred from Biological aspect of Ancestor more info
enables chromatin binding	IDA Inferred from Direct Assay more info	PubMed
enables helicase activity	IEA Inferred from Electronic Annotation more info
enables hydrolase activity	IEA Inferred from Electronic Annotation more info
enables nucleosome array spacer activity	IBA Inferred from Biological aspect of Ancestor more info
enables nucleosome binding	IEA Inferred from Electronic Annotation more info
enables single-stranded DNA binding	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in cellular response to pheromone	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in chromatin organization	IEA Inferred from Electronic Annotation more info
involved_in chromatin remodeling	IDA Inferred from Direct Assay more info	PubMed
involved_in chromatin remodeling	IEA Inferred from Electronic Annotation more info
involved_in chromatin remodeling	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in heterochromatin formation	IBA Inferred from Biological aspect of Ancestor more info
involved_in negative regulation of antisense RNA transcription	IGI Inferred from Genetic Interaction more info	PubMed
involved_in negative regulation of transcription by RNA polymerase II	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of transcription by RNA polymerase II	IBA Inferred from Biological aspect of Ancestor more info
involved_in rDNA heterochromatin formation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of DNA-templated transcription	IEA Inferred from Electronic Annotation more info
involved_in subtelomeric heterochromatin formation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in termination of RNA polymerase II transcription	IGI Inferred from Genetic Interaction more info	PubMed

Component	Evidence Code	Pubs
part_of CHRAC	IDA Inferred from Direct Assay more info	PubMed
located_in chromatin	IBA Inferred from Biological aspect of Ancestor more info
located_in chromosome, telomeric region	IEA Inferred from Electronic Annotation more info
located_in nuclear microtubule	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	HDA more info	PubMed
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: DNA translocase

NP_014948.1

ATP-dependent DNA translocase involved in chromatin remodeling; ATPase component that, with Itc1p, forms a complex required for repression of a-specific genes, INO1, and early meiotic genes during mitotic growth; exhibits basal levels of chromatin binding throughout the genome as well as interacts with sequence-specific factors to accurately position nucleosomes in chromatin; targeted by Ume6p- and Sua7p-dependent DNA looping to many loci genome-wide

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001147.6 Reference assembly

Range

884514..887876

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001183724.1 → NP_014948.1 TPA: DNA translocase [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_014948.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6W303, Q08773

UniProtKB/TrEMBL

G2WNH0, N1NVZ3

Conserved Domains (1) summary

PLN03142
Location:1 → 1093

PLN03142; Probable chromatin-remodeling complex ATPase chain; Provisional