ARF1 Arf family GTPase ARF1 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: ARF1
Official Full Name: Arf family GTPase ARF1
Primary source: SGD:S000002351
Locus tag: YDL192W
See related: AllianceGenome:SGD:S000002351; FungiDB:YDL192W; VEuPathDB:YDL192W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables GTPase activity. Involved in several processes, including Golgi vesicle transport; protein localization to Golgi membrane; and regulation of Golgi organization. Acts upstream of or within positive regulation of mitochondrial fission; positive regulation of mitochondrial fusion; and regulation of fatty acid metabolic process. Located in cytosol. Is active in Golgi apparatus and organelle membrane contact site. Human ortholog(s) of this gene implicated in periventricular nodular heterotopia. Orthologous to several human genes including ARF1 (ADP ribosylation factor 1) and ARF3 (ADP ribosylation factor 3). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See ARF1 in Genome Data Viewer

Location:: chromosome: IV

Exon count:: 1

Sequence:: Chromosome: IV; NC_001136.10 (116321..116866)

Chromosome IV - NC_001136.10 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001136.10

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

An interaction between beta'-COP and the ArfGAP, Glo3, maintains post-Golgi cargo recycling.
Title: An interaction between β'-COP and the ArfGAP, Glo3, maintains post-Golgi cargo recycling.
Structural basis for activation of Arf1 at the Golgi complex.
Title: Structural basis for activation of Arf1 at the Golgi complex.
The GTPase Arf1 Is a Determinant of Yeast Vps13 Localization to the Golgi Apparatus.
Title: The GTPase Arf1 Is a Determinant of Yeast Vps13 Localization to the Golgi Apparatus.
Arf1 directly recruits the Pik1-Frq1 PI4K complex to regulate the final stages of Golgi maturation.
Title: Arf1 directly recruits the Pik1-Frq1 PI4K complex to regulate the final stages of Golgi maturation.
Arf1 orchestrates Rab GTPase conversion at the trans-Golgi network.
Title: Arf1 orchestrates Rab GTPase conversion at the trans-Golgi network.
The Glo3 GAP crystal structure supports the molecular niche model for ArfGAPs in COPI coats.
Title: The Glo3 GAP crystal structure supports the molecular niche model for ArfGAPs in COPI coats.
Date show that ADP-ribosylation factor 1 (ARF1) deletion alters Vps74 distribution along Golgi stacks.
Title: Vps74p controls Golgi size in an Arf1-dependent manner.
Arf1 and the exomer cargo adaptor coordinate cargo sorting into subset of secretory vesicle carriers in the model organism Saccharomyces cerevisiae. Here, we detail the assays we used to explore the cooperative action of Arf1 and exomer to bind and remodel membranes.
Title: Analysis of Arf1 GTPase-Dependent Membrane Binding and Remodeling Using the Exomer Secretory Vesicle Cargo Adaptor.
The DCB/HUS domain amplifies the ability of Sec7 to activate Arf1 on the membrane surface by facilitating membrane insertion of the Arf1 amphipathic helix.
Title: The Sec7 N-terminal regulatory domains facilitate membrane-proximal activation of the Arf1 GTPase.
Arf1 is involved in mitochondrial homeostasis and dynamics, independent of its role in vesicular traffic.
Title: The small GTPase Arf1 modulates mitochondrial morphology and function.

Submit: New GeneRIF Correction See all GeneRIFs (18)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables GTP binding	IBA Inferred from Biological aspect of Ancestor more info
enables GTP binding	IEA Inferred from Electronic Annotation more info
enables GTPase activity	IDA Inferred from Direct Assay more info	PubMed
enables GTPase activity	IEA Inferred from Electronic Annotation more info
enables hydrolase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in Golgi to plasma membrane transport	IGI Inferred from Genetic Interaction more info	PubMed
involved_in Golgi to plasma membrane transport	IPI Inferred from Physical Interaction more info	PubMed
involved_in endoplasmic reticulum to Golgi vesicle-mediated transport	IDA Inferred from Direct Assay more info	PubMed
involved_in intracellular protein transport	IBA Inferred from Biological aspect of Ancestor more info
involved_in macroautophagy	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within positive regulation of mitochondrial fission	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within positive regulation of mitochondrial fusion	IGI Inferred from Genetic Interaction more info	PubMed
involved_in protein localization to Golgi membrane	IDA Inferred from Direct Assay more info	PubMed
involved_in protein transport	IEA Inferred from Electronic Annotation more info
involved_in regulation of Golgi organization	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within regulation of fatty acid metabolic process	IGI Inferred from Genetic Interaction more info	PubMed
involved_in vesicle-mediated transport	IBA Inferred from Biological aspect of Ancestor more info
involved_in vesicle-mediated transport	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in Golgi apparatus	HDA more info	PubMed
is_active_in Golgi apparatus	IDA Inferred from Direct Assay more info	PubMed
located_in Golgi apparatus	IDA Inferred from Direct Assay more info	PubMed
located_in Golgi apparatus	IEA Inferred from Electronic Annotation more info
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytosol	HDA more info	PubMed
is_active_in organelle membrane contact site	IDA Inferred from Direct Assay more info	PubMed
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info

General protein information

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Preferred Names: Arf family GTPase ARF1

NP_010089.1

EC 3.6.5.2

ADP-ribosylation factor; GTPase of the Ras superfamily that regulates coated vesicle formation during intracellular trafficking within the Golgi; regulates mitochondrial fission, fusion and function, controlling the flow of fatty acids and metabolites from lipid droplets to peroxisomes and mitochondria; localizes to the Golgi apparatus and the active form to ER and lipid droplets; arf1 arf2 double null mutation is complemented by human ARF1, ARF4, ARF5, or ARF6

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001136.10 Reference assembly

Range

116321..116866

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001180252.1 → NP_010089.1 TPA: Arf family GTPase ARF1 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_010089.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VRG1, P11076

UniProtKB/TrEMBL

A6ZXE0, B3LH74, C7GV54, C8Z6B1, G2WBX4, N1P6V2

Conserved Domains (1) summary

cl38936
Location:5 → 179

P-loop_NTPase; P-loop containing Nucleoside Triphosphate Hydrolases