WEE1 WEE1 G2 checkpoint kinase [Homo sapiens (human)] - Gene

Summary

Official Symbol: WEE1provided by HGNC
Official Full Name: WEE1 G2 checkpoint kinaseprovided by HGNC
Primary source: HGNC:HGNC:12761
See related: Ensembl:ENSG00000166483 MIM:193525; AllianceGenome:HGNC:12761
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Homo sapiens
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as: WEE1A; WEE1hu
Summary: This gene encodes a nuclear protein, which is a tyrosine kinase belonging to the Ser/Thr family of protein kinases. This protein catalyzes the inhibitory tyrosine phosphorylation of CDC2/cyclin B kinase, and appears to coordinate the transition between DNA replication and mitosis by protecting the nucleus from cytoplasmically activated CDC2 kinase. [provided by RefSeq, Jul 2008]
Expression: Ubiquitous expression in gall bladder (RPKM 23.7), esophagus (RPKM 19.4) and 22 other tissues See more
Orthologs: mouse all
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Genomic context

Location:: 11p15.4

Exon count:: 13

Annotation release	Status	Assembly	Chr	Location
RS_2024_08	current	GRCh38.p14 (GCF_000001405.40)	11	NC_000011.10 (9573670..9589985)
RS_2024_08	current	T2T-CHM13v2.0 (GCF_009914755.1)	11	NC_060935.1 (9657890..9674136)
RS_2024_09	previous assembly	GRCh37.p13 (GCF_000001405.25)	11	NC_000011.9 (9595217..9611532)

Chromosome 11 - NC_000011.10 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: HPA RNA-seq normal tissues
Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
BioProject: PRJEB4337
Publication: PMID 24309898
Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Identification of DNA methylation-regulated WEE1 with potential implications in prognosis and immunotherapy for low-grade glioma.
Title: Identification of DNA methylation-regulated WEE1 with potential implications in prognosis and immunotherapy for low-grade glioma.
In silico and structure-based evaluation of deleterious mutations identified in human Chk1, Chk2, and Wee1 protein kinase.
Title: In silico and structure-based evaluation of deleterious mutations identified in human Chk1, Chk2, and Wee1 protein kinase.
Circulating RNA ZFR promotes hepatocellular carcinoma cell proliferation and epithelial-mesenchymal transition process through miR-624-3p/WEE1 axis.
Title: Circulating RNA ZFR promotes hepatocellular carcinoma cell proliferation and epithelial-mesenchymal transition process through miR-624-3p/WEE1 axis.
E2F8 exerts cancer-promoting effects by transcriptionally activating RRM2 and E2F8 knockdown synergizes with WEE1 inhibition in suppressing lung adenocarcinoma.
Title: E2F8 exerts cancer-promoting effects by transcriptionally activating RRM2 and E2F8 knockdown synergizes with WEE1 inhibition in suppressing lung adenocarcinoma.
Wee1 promotes cell proliferation and imatinib resistance in chronic myeloid leukemia via regulating DNA damage repair dependent on ATM-gammaH2AX-MDC1.
Title: Wee1 promotes cell proliferation and imatinib resistance in chronic myeloid leukemia via regulating DNA damage repair dependent on ATM-γH2AX-MDC1.
ODF2L acts as a synthetic lethal partner with WEE1 inhibition in epithelial ovarian cancer models.
Title: ODF2L acts as a synthetic lethal partner with WEE1 inhibition in epithelial ovarian cancer models.
Mitotic Checkpoints and the Role of WEE1 Inhibition in Head and Neck Squamous Cell Carcinoma.
Title: Mitotic Checkpoints and the Role of WEE1 Inhibition in Head and Neck Squamous Cell Carcinoma.
Verbascoside enhances radiosensitivity of hepatocellular carcinoma cells through regulating miR-101-3p/Wee1 axis.
Title: Verbascoside enhances radiosensitivity of hepatocellular carcinoma cells through regulating miR-101-3p/Wee1 axis.
Inhibiting WEE1 and IKK-RELA Crosstalk Overcomes TNFalpha Resistance in Head and Neck Cancers.
Title: Inhibiting WEE1 and IKK-RELA Crosstalk Overcomes TNFα Resistance in Head and Neck Cancers.
WEE1 kinase is a therapeutic vulnerability in CIC-DUX4 undifferentiated sarcoma.
Title: WEE1 kinase is a therapeutic vulnerability in CIC-DUX4 undifferentiated sarcoma.

Submit: New GeneRIF Correction See all GeneRIFs (132)

Phenotypes

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Find tests for this gene in the NIH Genetic Testing Registry (GTR)

Review eQTL and phenotype association data in this region using PheGenI

Variation

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See variants in ClinVar

See studies and variants in dbVar

See Variation Viewer (GRCh37.p13)

See Variation Viewer (GRCh38)

HIV-1 interactions

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Protein interactions

Protein	Gene	Interaction	Pubs
Vpr	vpr	Prolonged cell cycle arrest in the G2 phase induced by HIV-1 Vpr results in the depletion of Wee1 kinase	PubMed
	vpr	HIV-1 Vpr binds to Wee1. Amino acids 291-369 in Wee1 are required for the binding between Vpr and Wee1. Vpr stimulates Wee1 kinase activity	PubMed
	vpr	HIV-1 Vpr-induced cell cycle arrest results in increased levels of Wee-1 kinase in synchronized HeLa cells; G2 arrest by Vpr is correlated with delayed degradation of Wee-1, and Cdc2 activity is reduced at G2/M in the presence of Vpr	PubMed
	vpr	HIV-1 Vpr induced cell cycle arrest in the G2 phase is mediated predominately through the Wee1 kinase in fission yeast	PubMed
	vpr	Overexpression of Wee-1 kinase suppresses HIV-1 Vpr-mediated apoptosis	PubMed
	vpr	Rupture of HIV-1 Vpr-induced nuclear envelope herniations produces transient loss of the subcellular compartmentalization of Wee1, Cdc25C, cyclin B1, and presumably other soluble cellular components, resulting in their release into the cytoplasm	PubMed

Go to the HIV-1, Human Interaction Database

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

D8S2279 (e-PCR)
- UniSTS:473907

D10S16 (e-PCR)
- UniSTS:155756

RH18120 (e-PCR)
- UniSTS:41081

D11S3727 (e-PCR)
- UniSTS:152957

WEE1 (e-PCR)
- UniSTS:10381

MARC_17360-17361:1021651098:1 (e-PCR)
- UniSTS:268284

Gene Ontology Provided by GOA

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables histone H2AXY142 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3Y41 kinase activity	IEA Inferred from Electronic Annotation more info
enables magnesium ion binding	IEA Inferred from Electronic Annotation more info
enables non-membrane spanning protein tyrosine kinase activity	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein tyrosine kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables protein tyrosine kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables protein tyrosine kinase activity	TAS Traceable Author Statement more info

Process	Evidence Code	Pubs
involved_in G2/M transition of mitotic cell cycle	TAS Traceable Author Statement more info
involved_in cell division	IEA Inferred from Electronic Annotation more info
involved_in chromatin remodeling	IEA Inferred from Electronic Annotation more info
involved_in establishment of cell polarity	IEA Inferred from Electronic Annotation more info
involved_in microtubule cytoskeleton organization	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of G1/S transition of mitotic cell cycle	TAS Traceable Author Statement more info
involved_in negative regulation of G2/M transition of mitotic cell cycle	IBA Inferred from Biological aspect of Ancestor more info
involved_in negative regulation of G2/M transition of mitotic cell cycle	IDA Inferred from Direct Assay more info	PubMed
involved_in neuron projection morphogenesis	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of DNA replication	TAS Traceable Author Statement more info

Component	Evidence Code	Pubs
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleolus	IDA Inferred from Direct Assay more info
located_in nucleoplasm	TAS Traceable Author Statement more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
is_active_in nucleus	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: wee1-like protein kinase

Names: WEE1 homolog; WEE1+ homolog; protein kinase; wee1A kinase

NP_001137448.1

EC 2.7.10.2

NP_003381.1

EC 2.7.10.2

XP_047283495.1

EC 2.7.10.2

XP_054225826.1

EC 2.7.10.2

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001143976.2 → NP_001137448.1 wee1-like protein kinase isoform 2

See identical proteins and their annotated locations for NP_001137448.1

Status: REVIEWED

Description

Transcript Variant: This variant (2) has a different first exon and 5' UTR, compared to variant 1. This difference causes translation initiation from an in-frame downstream AUG and an isoform (2) with a shorter N-terminus, compared to isoform 1.

Source sequence(s)

AC011979, AK122837, BQ024511

Consensus CDS

CCDS44536.1

UniProtKB/Swiss-Prot

P30291

Related

ENSP00000299613.5, ENST00000299613.10

Conserved Domains (2) summary

cd14138
Location:79 → 354

PTKc_Wee1a; Catalytic domain of the Protein Tyrosine Kinase, Wee1a

pfam00069
Location:85 → 355

Pkinase; Protein kinase domain
NM_003390.4 → NP_003381.1 wee1-like protein kinase isoform 1

See identical proteins and their annotated locations for NP_003381.1

Status: REVIEWED

Description

Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).

Source sequence(s)

AC011979, AC122179, BC051831, BQ024511, U10564

Consensus CDS

CCDS7800.1

UniProtKB/Swiss-Prot

B3KVE1, D3DQV0, P30291

UniProtKB/TrEMBL

Q6NSL2, Q86V29

Related

ENSP00000402084.2, ENST00000450114.7

Conserved Domains (2) summary

cd14138
Location:293 → 568

PTKc_Wee1a; Catalytic domain of the Protein Tyrosine Kinase, Wee1a

pfam00069
Location:299 → 569

Pkinase; Protein kinase domain