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    alg44 alginate biosynthesis protein Alg44 [ Pseudomonas aeruginosa PAO1 ]

    Gene ID: 878770, updated on 24-Aug-2024

    Summary

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    Gene symbol
    alg44
    Gene description
    alginate biosynthesis protein Alg44
    Locus tag
    PA3542
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Pseudomonas aeruginosa PAO1 (strain: PAO1)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales; Pseudomonadaceae; Pseudomonas
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    Genomic context

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    Sequence:
    NC_002516.2 (3965842..3967011)

    NC_002516.2Genomic Context describing neighboring genes Neighboring gene GDP-mannose 6-dehydrogenase AlgD Neighboring gene glycosyltransferase alg8 Neighboring gene alginate biosynthesis protein AlgK Neighboring gene alginate production protein AlgE Neighboring gene alginate-c5-mannuronan-epimerase AlgG

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_002516.2

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. The second messenger bis-(3'-5')-cyclic-GMP and its PilZ domain-containing receptor Alg44 are required for alginate biosynthesis in Pseudomonas aeruginosa. Merighi M, et al. Mol Microbiol, 2007 Aug. PMID 17645452
    2. Activation Mechanism and Cellular Localization of Membrane-Anchored Alginate Polymerase in Pseudomonas aeruginosa. Moradali MF, et al. Appl Environ Microbiol, 2017 May 1. PMID 28258142, Free PMC Article
    3. Alg44, a unique protein required for alginate biosynthesis in Pseudomonas aeruginosa. Remminghorst U, et al. FEBS Lett, 2006 Jul 10. PMID 16797016
    4. Membrane topology and roles of Pseudomonas aeruginosa Alg8 and Alg44 in alginate polymerization. Oglesby LL, et al. Microbiology (Reading), 2008 Jun. PMID 18524915, Free PMC Article
    5. Pseudomonas Genome Database: facilitating user-friendly, comprehensive comparisons of microbial genomes. Winsor GL, et al. Nucleic Acids Res, 2009 Jan. PMID 18978025, Free PMC Article

    See all (14) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. The Alg44 alginate copolymerase constituted a stable dimer, with its periplasmic domains required for protein localization and alginate polymerization and modification.
      Title: Activation Mechanism and Cellular Localization of Membrane-Anchored Alginate Polymerase in Pseudomonas aeruginosa.
    2. Alg44 may act as a co-polymerase in concert with Alg8, the major GT, and that both inner-membrane proteins are required in vivo for the polymerization reaction leading to alginate production
      Title: Membrane topology and roles of Pseudomonas aeruginosa Alg8 and Alg44 in alginate polymerization.
    3. Alg44 is a single pass transmembrane protein containing a cytoplasmic PilZ domain that binds the second messenger c-di-GMP in vitro. Point mutations abolishing binding in vitro also abolish function in vivo. Alg44 is required for alginate biosynthesis.
      Title: The second messenger bis-(3'-5')-cyclic-GMP and its PilZ domain-containing receptor Alg44 are required for alginate biosynthesis in Pseudomonas aeruginosa.
    4. Alg44 might be directly involved in alginate polymerization presumably by exerting a regulatory function.
      Title: Alg44, a unique protein required for alginate biosynthesis in Pseudomonas aeruginosa.
    Submit: New GeneRIF Correction

    Pathways from PubChem

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    General gene information

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    Homology

    Gene Ontology Provided by PseudoCAP

    Function Evidence Code Pubs
    enables long-chain fatty acid-CoA ligase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Process Evidence Code Pubs
    acts_upstream_of_or_within alginic acid biosynthetic process IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    acts_upstream_of_or_within alginic acid biosynthetic process IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within single-species biofilm formation IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in Gram-negative-bacterium-type cell wall IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    alginate biosynthesis protein Alg44
    NP_252232.1
    • Product name confidence: class 1 (Function experimentally demonstrated in P. aeruginosa)

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_002516.2 Reference assembly

      Range
      3965842..3967011
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_252232.1 alginate biosynthesis protein Alg44 [Pseudomonas aeruginosa PAO1]

      See identical proteins and their annotated locations for NP_252232.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A3M5DXB9
      Conserved Domains (3) summary
      pfam07238
      Location:16 → 115
      PilZ; PilZ domain
      pfam13437
      Location:262 → 357
      HlyD_3; HlyD family secretion protein
      cl11404
      Location:204 → 291
      Biotinyl_lipoyl_domains; Biotinyl_lipoyl_domains are present in biotin-dependent carboxylases/decarboxylases, the dihydrolipoyl acyltransferase component (E2) of 2-oxo acid dehydrogenases, and the H-protein of the glycine cleavage system (GCS). These domains transport CO2, acyl, ...
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q9HY69.1

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