FAB1 1-phosphatidylinositol-3-phosphate 5-kinase [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: FAB1
Official Full Name: 1-phosphatidylinositol-3-phosphate 5-kinase
Primary source: SGD:S000001915
Locus tag: YFR019W
See related: AllianceGenome:SGD:S000001915; FungiDB:YFR019W; VEuPathDB:YFR019W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: SVL7
Summary: Enables 1-phosphatidylinositol-3-phosphate 5-kinase activity and phosphatidylinositol-3-phosphate binding activity. Involved in phosphatidylinositol phosphate biosynthetic process. Located in endosome membrane and fungal-type vacuole membrane. Part of PAS complex. Human ortholog(s) of this gene implicated in Fleck corneal dystrophy. Orthologous to human PIKFYVE (phosphoinositide kinase, FYVE-type zinc finger containing). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See FAB1 in Genome Data Viewer

Location:: chromosome: VI

Exon count:: 1

Sequence:: Chromosome: VI; NC_001138.5 (184502..191338)

Chromosome VI - NC_001138.5 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001138.5

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

TORC1 Determines Fab1 Lipid Kinase Function at Signaling Endosomes and Vacuoles.
Title: TORC1 Determines Fab1 Lipid Kinase Function at Signaling Endosomes and Vacuoles.
data suggest that Ivy1 modulates Fab1-mediated PI-3,5-P2 synthesis during membrane stress and may allow adjustment of the vacuole membrane environment.
Title: Control of vacuole membrane homeostasis by a resident PI-3,5-kinase inhibitor.
Pho85/CDK5 provides protection against hyperosmotic stress and acts before long-term adaptation provided by Hog1. This protection requires the vacuolar/endolysosomal signaling lipid PI3,5P2 We show that Pho85/CDK5 directly phosphorylates and positively regulates the PI3P-5 kinase Fab1/PIKfyve complex and provide evidence that this regulation is conserved in mammalian cells.
Title: Early protection to stress mediated by CDK-dependent PI3,5P(2) signaling from the vacuole/lysosome.
ATG18 and FAB1 are involved in dehydration stress tolerance in Saccharomyces cerevisiae.
Title: ATG18 and FAB1 are involved in dehydration stress tolerance in Saccharomyces cerevisiae.
Data suggest a model in which PtdIns(3)P 5-kinase protein Fab1/PIKfyve is dispensable to maintain vacuolar/lysosomal acidification.
Title: The Fab1/PIKfyve phosphoinositide phosphate kinase is not necessary to maintain the pH of lysosomes and of the yeast vacuole.
The crystal structure now unravels the presence of two distinct phosphoinositide-binding sites at the circumference of the seven bladed beta-propeller showing PtdINS3P and PtdIns(3,5)P2 proteins
Title: It takes two to tango: PROPPINs use two phosphoinositide-binding sites.
Vac14 protein multimerization is a prerequisite step for Fab1 protein complex assembly and funct
Title: Vac14 protein multimerization is a prerequisite step for Fab1 protein complex assembly and function.
Fab1 phosphoinositide kinase signaling complex assembly requires the Fig4 phosphoinositide phosphatase
Title: Assembly of a Fab1 phosphoinositide kinase signaling complex requires the Fig4 phosphoinositide phosphatase.
Atg18p is likely a PtdIns(3,5)P(2)"sensor," acting as an effector to remodel membranes as well as regulating its synthesis via feedback that might involve Vac7p
Title: Atg18 regulates organelle morphology and Fab1 kinase activity independent of its membrane recruitment by phosphatidylinositol 3,5-bisphosphate.
Results show that Fab1p is required for AP-1-dependent process for trafficking of endogenously ubiquitylated cargoes to the vacuole lumen in S. cerevisiae.
Title: Fab1p and AP-1 are required for trafficking of endogenously ubiquitylated cargoes to the vacuole lumen in S. cerevisiae.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables 1-phosphatidylinositol-3-phosphate 5-kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables 1-phosphatidylinositol-3-phosphate 5-kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables 1-phosphatidylinositol-3-phosphate 5-kinase activity	IEA Inferred from Electronic Annotation more info
enables 1-phosphatidylinositol-3-phosphate 5-kinase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables kinase activity	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables phosphatidylinositol-3-phosphate binding	IDA Inferred from Direct Assay more info	PubMed
enables phosphatidylinositol-3-phosphate binding	IEA Inferred from Electronic Annotation more info
enables zinc ion binding	RCA inferred from Reviewed Computational Analysis more info	PubMed

Process	Evidence Code	Pubs
involved_in phosphatidylinositol metabolic process	IEA Inferred from Electronic Annotation more info
involved_in phosphatidylinositol phosphate biosynthetic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in phosphatidylinositol phosphate biosynthetic process	IDA Inferred from Direct Assay more info	PubMed
involved_in phosphatidylinositol phosphate biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in vacuole organization	IBA Inferred from Biological aspect of Ancestor more info

Component	Evidence Code	Pubs
part_of PAS complex	IDA Inferred from Direct Assay more info	PubMed
part_of PAS complex	IPI Inferred from Physical Interaction more info	PubMed
located_in bounding membrane of organelle	IEA Inferred from Electronic Annotation more info
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in endosome	IEA Inferred from Electronic Annotation more info
is_active_in endosome membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in endosome membrane	IDA Inferred from Direct Assay more info	PubMed
located_in endosome membrane	IEA Inferred from Electronic Annotation more info
is_active_in fungal-type vacuole membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in fungal-type vacuole membrane	IDA Inferred from Direct Assay more info	PubMed
located_in membrane	IEA Inferred from Electronic Annotation more info
located_in mitochondrion	HDA more info	PubMed
located_in vacuolar membrane	IEA Inferred from Electronic Annotation more info
located_in vacuole	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: 1-phosphatidylinositol-3-phosphate 5-kinase

NP_116674.2

EC 2.7.1.150

1-phosphatidylinositol-3-phosphate 5-kinase; vacuolar membrane kinase that generates phosphatidylinositol (3,5)P2, which is involved in vacuolar sorting and homeostasis

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001138.5 Reference assembly

Range

184502..191338

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001179984.2 → NP_116674.2 TPA: 1-phosphatidylinositol-3-phosphate 5-kinase [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_116674.2

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VTQ0, P34756

UniProtKB/TrEMBL

B3LUM8

Conserved Domains (4) summary

PHA03255
Location:64 → 145

PHA03255; BDLF3; Provisional

COG5253
Location:1597 → 2278

MSS4; Phosphatidylinositol-4-phosphate 5-kinase [Signal transduction mechanisms]

cd03334
Location:797 → 1056

Fab1_TCP; TCP-1 like domain of the eukaryotic phosphatidylinositol 3-phosphate (PtdIns3P) 5-kinase Fab1. Fab1p is important for vacuole size regulation, presumably by modulating PtdIns(3,5)P2 effector activity. In the human homolog p235/PIKfyve deletion of this ...

cl28890
Location:236 → 296

FYVE_like_SF; FYVE domain like superfamily