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    MLC1 Mlc1p [ Saccharomyces cerevisiae S288C ]

    Gene ID: 852772, updated on 9-Dec-2024

    Summary

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    Official Symbol
    MLC1
    Official Full Name
    Mlc1p
    Primary source
    SGD:S000003074
    Locus tag
    YGL106W
    See related
    AllianceGenome:SGD:S000003074; FungiDB:YGL106W; VEuPathDB:YGL106W
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Saccharomyces cerevisiae S288C (strain: S288C)
    Lineage
    Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
    Summary
    Enables myosin II heavy chain binding activity and myosin V binding activity. Involved in protein localization to cell division site involved in mitotic actomyosin contractile ring assembly and vesicle targeting. Located in cellular bud neck contractile ring; cellular bud tip; and vesicle. Part of MIH complex and myosin II complex. Human ortholog(s) of this gene implicated in cardiomyopathy; congenital myopathy 14; familial atrial fibrillation; and hypertrophic cardiomyopathy 8. Orthologous to several human genes including MYL4 (myosin light chain 4); MYL6 (myosin light chain 6); and MYL6B (myosin light chain 6B). [provided by Alliance of Genome Resources, Dec 2024]
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    Genomic context

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    See MLC1 in Genome Data Viewer
    Location:
    chromosome: VII
    Exon count:
    1
    Sequence:
    Chromosome: VII; NC_001139.9 (306560..307009)

    Chromosome VII - NC_001139.9Genomic Context describing neighboring genes Neighboring gene uncharacterized protein Neighboring gene Rmd9p Neighboring gene Arc1p Neighboring gene putative sugar transporter

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_001139.9

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Assignment of the 1H, 13C and 15N resonances of Mlc1p from Saccharomices cerevisiae. Melino S, et al. J Biomol NMR, 2005 Apr. PMID 15929007
    2. N-lobe dynamics of myosin light chain dictates its mode of interaction with myosin V IQ1. Amata I, et al. Biochemistry, 2008 Nov 25. PMID 18975975
    3. The class V myosin motor protein, Myo2, plays a major role in mitochondrial motility in Saccharomyces cerevisiae. Altmann K, et al. J Cell Biol, 2008 Apr 7. PMID 18391073, Free PMC Article
    4. Crystallization, X-ray characterization and selenomethionine phasing of Mlc1p bound to IQ motifs from myosin V. Terrak M, et al. Acta Crystallogr D Biol Crystallogr, 2002 Oct. PMID 12351846
    5. A molecular dynamics study and free energy analysis of complexes between the Mlc1p protein and two IQ motif peptides. Ganoth A, et al. Biophys J, 2006 Oct 1. PMID 16844751, Free PMC Article

    See all (74) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Myo1 plays a major role in the septin-dependent Mlc1 localization to the division site before cytokinesis, whereas the formin Bni1 plays a major role in the actin filament-dependent Mlc1 localization during cytokinesis.
      Title: Myosin‑II heavy chain and formin mediate the targeting of myosin essential light chain to the division site before and during cytokinesis.
    2. Here, we show that the rigid conformation of the loop connecting the two EF-hand motifs of the Mlc1p N-lobe explains its differential ability to interact with myosin V IQ1.
      Title: N-lobe dynamics of myosin light chain dictates its mode of interaction with myosin V IQ1.
    3. myosin light chain 1 (Mlc1p), required for vesicle delivery at the mother-bud neck during cytokinesis, binds to the Myo2p GTD in a region overlapping that of Ypt32p.
      Title: Ypt32p and Mlc1p bind within the vesicle binding region of the class V myosin Myo2p globular tail domain.
    4. Mlc1p/Myo2p interaction is stimulated by a signal that requires Sec2p and activation of Ypt32p
      Title: GTP drives myosin light chain 1 interaction with the class V myosin Myo2 IQ motifs via a Sec2 RabGEF-mediated pathway.
    5. NMR assignment of 1H, 13C and 15N resonances for Mlc1p from Saccharomices cerevisiae
      Title: Assignment of the 1H, 13C and 15N resonances of Mlc1p from Saccharomices cerevisiae.
    6. analysis of the interaction of Mlc1p with the first IQ motif of Myo2p
      Title: Structural basis for the interaction of the myosin light chain Mlc1p with the myosin V Myo2p IQ motifs.
    7. mrl1 is a mitochondrial factor for myo2p-dependent inheritance of mitochondria.
      Title: Mmr1p is a mitochondrial factor for Myo2p-dependent inheritance of mitochondria in the budding yeast.
    8. the 2-A resolution crystal structure of myosin light chain 1 (Mlc1p) bound to the IQ2-IQ3 fragment of Myo2p, a myosin V from Saccharomyces cerevisiae
      Title: Structure of the light chain-binding domain of myosin V.
    Submit: New GeneRIF Correction

    Pathways from PubChem

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    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by SGD

    Function Evidence Code Pubs
    enables calcium ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables myosin II heavy chain binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables myosin V binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in cell division IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in mitotic actomyosin contractile ring assembly IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in protein localization to cell division site involved in mitotic actomyosin contractile ring assembly IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    involved_in protein localization to cell division site involved in mitotic actomyosin contractile ring assembly IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in vesicle targeting IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    part_of MIH complex IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    part_of MIH complex IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    located_in cellular bud neck IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in cellular bud neck IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in cellular bud neck contractile ring IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in cellular bud tip IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in cellular bud tip IEA
    Inferred from Electronic Annotation
    more info
    		  
    part_of myosin II complex IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    part_of myosin II complex IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    part_of myosin complex IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in site of polarized growth IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in vesicle IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    Mlc1p
    NP_011409.1
    • Essential light chain for Myo1p; light chain for Myo2p; stabilizes Myo2p by binding to the neck region; interacts with Myo1p, Iqg1p, and Myo2p to coordinate formation and contraction of the actomyosin ring with targeted membrane deposition

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_001139.9 Reference assembly

      Range
      306560..307009
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_001180971.1 → NP_011409.1  TPA: Mlc1p [Saccharomyces cerevisiae S288C]

      See identical proteins and their annotated locations for NP_011409.1

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      D6VU40, P53141
      UniProtKB/TrEMBL
      A6ZU75, B3LHH0, B5VIM0, C7GM01, C8Z8E2, G2WDZ4, N1P3I0
      Conserved Domains (1) summary
      COG5126
      Location:1 → 149
      FRQ1; Ca2+-binding protein, EF-hand superfamily [Signal transduction mechanisms]
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    P53141.1

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