SLD3 Sld3p [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: SLD3
Official Full Name: Sld3p
Primary source: SGD:S000003081
Locus tag: YGL113W
See related: AllianceGenome:SGD:S000003081; FungiDB:YGL113W; VEuPathDB:YGL113W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables chromatin binding activity. Involved in DNA replication initiation; DNA unwinding involved in DNA replication; and double-strand break repair via break-induced replication. Located in chromatin and chromosome, centromeric region. Part of DNA replication preinitiation complex. [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See SLD3 in Genome Data Viewer

Location:: chromosome: VII

Exon count:: 1

Sequence:: Chromosome: VII; NC_001139.9 (295932..297938)

Chromosome VII - NC_001139.9 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001139.9

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Interaction of replication factor Sld3 and histone acetyl transferase Esa1 alleviates gene silencing and promotes the activation of late and dormant replication origins.
Title: Interaction of replication factor Sld3 and histone acetyl transferase Esa1 alleviates gene silencing and promotes the activation of late and dormant replication origins.
Sld3 binds specifically to DDK-phosphorylated peptides from two MCM subunits (Mcm4, 6) and then recruits Cdc45.
Title: Phosphopeptide binding by Sld3 links Dbf4-dependent kinase to MCM replicative helicase activation.
Here, the crystal structure of Sld7 in complex with Sld3 is presented. Sld7 comprises two structural domains.
Title: The quaternary structure of the eukaryotic DNA replication proteins Sld7 and Sld3.
The crystal structure of the central domain of Sld3 from Saccharomyces cerevisiae.
Title: Crystal structure of the homology domain of the eukaryotic DNA replication proteins Sld3/Treslin.
The low-abundance replication proteins Sld3, Sld7, and Cdc45 form a complex and associate with the early-firing origins in G1 phase in a manner that depends on Dbf4-dependent kinase (DDK), which is essential for the initiation of DNA replication.
Title: Origin association of Sld3, Sld7, and Cdc45 proteins is a key step for determination of origin-firing timing.
CDK substrates Sld3 and Sld2 and their binding partner Dpb11, together with the DDK subunit Dbf4 control S-phase length in budding yeast Saccharomyces cerevisiae limiting replication initiationst hrough their low abundance.
Title: Limiting replication initiation factors execute the temporal programme of origin firing in budding yeast.
origin single-stranded DNA releases Sld3 from Mcm2-7, allowing GINS to bind Mcm2-7.
Title: Origin single-stranded DNA releases Sld3 protein from the Mcm2-7 complex, allowing the GINS tetramer to bind the Mcm2-7 complex.
GINS (Psf1, Psf2, Psf3, and Sld5) and Sld3 compete with one another for Mcm2-7 and Cdc45 binding
Title: GINS and Sld3 compete with one another for Mcm2-7 and Cdc45 binding.
replication initiation protein Sld3 is phosphorylated by Rad53, and that this phosphorylation, along with phosphorylation of the Cdc7 kinase regulatory subunit Dbf4, blocks late origin firing in Saccharomyces cerevisiae
Title: Damage-induced phosphorylation of Sld3 is important to block late origin firing.
Sld2 and Sld3 are the minimal set of cyclin-dependent kinase targets required for DNA replication
Title: Phosphorylation of Sld2 and Sld3 by cyclin-dependent kinases promotes DNA replication in budding yeast.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables chromatin binding	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in DNA replication	IEA Inferred from Electronic Annotation more info
involved_in DNA replication initiation	IBA Inferred from Biological aspect of Ancestor more info
involved_in DNA replication initiation	IEA Inferred from Electronic Annotation more info
involved_in DNA replication initiation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in DNA unwinding involved in DNA replication	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in double-strand break repair via break-induced replication	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
part_of DNA replication preinitiation complex	IBA Inferred from Biological aspect of Ancestor more info
part_of DNA replication preinitiation complex	IPI Inferred from Physical Interaction more info	PubMed
located_in chromatin	IDA Inferred from Direct Assay more info	PubMed
located_in chromosome, centromeric region	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	HDA more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: Sld3p

NP_011402.3

Protein involved in the initiation of DNA replication; required for proper assembly of replication proteins at the origins of replication; interacts with the DDK-phosphorylated MCM complex and required for the recruitment of Cdc45p; localizes to nuclear foci that become diffuse upon DNA replication stress; homologous to the human Treslin/Ticrr protein

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001139.9 Reference assembly

Range

295932..297938

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001180978.3 → NP_011402.3 TPA: Sld3p [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_011402.3

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VU34, P53135

UniProtKB/TrEMBL

B3LHH6, C7GM07, C8Z8D7, N1P1T1

Conserved Domains (2) summary

pfam08639
Location:157 → 646

SLD3; DNA replication regulator SLD3

pfam18523
Location:5 → 121

Sld3_N; Sld3 N-terminal domain