PFK1 6-phosphofructokinase subunit alpha [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: PFK1
Official Full Name: 6-phosphofructokinase subunit alpha
Primary source: SGD:S000003472
Locus tag: YGR240C
See related: AllianceGenome:SGD:S000003472; FungiDB:YGR240C; VEuPathDB:YGR240C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Contributes to 6-phosphofructokinase activity. Involved in glycolytic process and regulation of intracellular pH. Located in mitochondrion. Part of 6-phosphofructokinase complex. Used to study glycogen storage disease. Human ortholog(s) of this gene implicated in glycogen storage disease VII. Orthologous to human PFKL (phosphofructokinase, liver type); PFKM (phosphofructokinase, muscle); and PFKP (phosphofructokinase, platelet). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See PFK1 in Genome Data Viewer

Location:: chromosome: VII

Exon count:: 1

Sequence:: Chromosome: VII; NC_001139.9 (970771..973734, complement)

Chromosome VII - NC_001139.9 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001139.9

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

PCK1 Deficiency Shortens the Replicative Lifespan of Saccharomyces cerevisiae through Upregulation of PFK1.
Title: PCK1 Deficiency Shortens the Replicative Lifespan of Saccharomyces cerevisiae through Upregulation of PFK1.
Regulation of Vacuolar H+-ATPase (V-ATPase) Reassembly by Glycolysis Flow in 6-Phosphofructo-1-kinase (PFK-1)-deficient Yeast Cells.
Title: Regulation of Vacuolar H+-ATPase (V-ATPase) Reassembly by Glycolysis Flow in 6-Phosphofructo-1-kinase (PFK-1)-deficient Yeast Cells.
stability and function of the metabolon composed of H+ATPase and glycolytic components can be compromised by either loss of required PFK-1 binding (G820R) or loss of pump protein (R807Q)
Title: Human H+ATPase a4 subunit mutations causing renal tubular acidosis reveal a role for interaction with phosphofructokinase-1.
Pfk1 underwent a large conformational change upon Mg-ATP binding arising from a reorientation of the alpha- and beta-subunits in the dimers.
Title: The structure of the ATP-bound state of S. cerevisiae phosphofructokinase determined by cryo-electron microscopy.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables 6-phosphofructokinase activity	IEA Inferred from Electronic Annotation more info
contributes_to 6-phosphofructokinase activity	IGI Inferred from Genetic Interaction more info	PubMed
contributes_to 6-phosphofructokinase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables fructose-6-phosphate binding	IBA Inferred from Biological aspect of Ancestor more info
enables kinase activity	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in canonical glycolysis	IBA Inferred from Biological aspect of Ancestor more info
involved_in fructose 1,6-bisphosphate metabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in fructose 6-phosphate metabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in fructose 6-phosphate metabolic process	IEA Inferred from Electronic Annotation more info
involved_in glycolytic process	IDA Inferred from Direct Assay more info	PubMed
involved_in glycolytic process	IEA Inferred from Electronic Annotation more info
involved_in glycolytic process	IGI Inferred from Genetic Interaction more info	PubMed
involved_in glycolytic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of intracellular pH	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
part_of 6-phosphofructokinase complex	IBA Inferred from Biological aspect of Ancestor more info
part_of 6-phosphofructokinase complex	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	HDA more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in mitochondrial outer membrane	IEA Inferred from Electronic Annotation more info
is_active_in mitochondrion	IBA Inferred from Biological aspect of Ancestor more info
located_in mitochondrion	IDA Inferred from Direct Assay more info	PubMed
located_in mitochondrion	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: 6-phosphofructokinase subunit alpha

NP_011756.1

EC 2.7.1.11

Alpha subunit of heterooctameric phosphofructokinase; involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001139.9 Reference assembly

Range

970771..973734 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001181369.1 → NP_011756.1 TPA: 6-phosphofructokinase subunit alpha [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_011756.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VV20, P16861

UniProtKB/TrEMBL

A6ZUP8, B3LI00, C7GNI9, C8Z9D1, G2WEW6, N1P2M1

Conserved Domains (2) summary

TIGR02478
Location:206 → 972

6PF1K_euk; 6-phosphofructokinase, eukaryotic type

pfam18468
Location:8 → 101

Pfk_N; Phosphofructokinase N-terminal domain yeast