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    ASI1 putative ubiquitin-protein ligase ASI1 [ Saccharomyces cerevisiae S288C ]

    Gene ID: 855147, updated on 9-Dec-2024

    Summary

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    Official Symbol
    ASI1
    Official Full Name
    putative ubiquitin-protein ligase ASI1
    Primary source
    SGD:S000004725
    Locus tag
    YMR119W
    See related
    AllianceGenome:SGD:S000004725; FungiDB:YMR119W; VEuPathDB:YMR119W
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Saccharomyces cerevisiae S288C (strain: S288C)
    Lineage
    Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
    Summary
    Enables ubiquitin-protein transferase activity. Involved in cellular response to amino acid stimulus and proteasome-mediated ubiquitin-dependent protein catabolic process. Located in nuclear inner membrane. Part of Asi complex. [provided by Alliance of Genome Resources, Dec 2024]
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    Genomic context

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    See ASI1 in Genome Data Viewer
    Location:
    chromosome: XIII
    Exon count:
    1
    Sequence:
    Chromosome: XIII; NC_001145.3 (505333..507207)

    Chromosome XIII - NC_001145.3Genomic Context describing neighboring genes Neighboring gene protein SHH3 Neighboring gene tRNA-Leu Neighboring gene bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase ADE17 Neighboring gene ribosomal 60S subunit protein L15B

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_001145.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Inner Nuclear Membrane Asi Ubiquitin Ligase Catalytic Subunits Asi1p and Asi3p, but not Asi2p, confer resistance to aminoglycoside hygromycin B in Saccharomyces cerevisiae. Woodruff KA, et al. MicroPubl Biol, 2021 Jun 1. PMID 34095778, Free PMC Article
    2. Mapping genetic variants underlying differences in the central nitrogen metabolism in fermenter yeasts. Jara M, et al. PLoS One, 2014. PMID 24466135, Free PMC Article
    3. Asi1 is an inner nuclear membrane protein that restricts promoter access of two latent transcription factors. Boban M, et al. J Cell Biol, 2006 Jun 5. PMID 16735580, Free PMC Article
    4. The N-terminal regulatory domain of Stp1p is modular and, fused to an artificial transcription factor, confers full Ssy1p-Ptr3p-Ssy5p sensor control. Andréasson C, et al. Mol Cell Biol, 2004 Sep. PMID 15314160, Free PMC Article
    5. Inner nuclear membrane proteins Asi1, Asi2, and Asi3 function in concert to maintain the latent properties of transcription factors Stp1 and Stp2. Zargari A, et al. J Biol Chem, 2007 Jan 5. PMID 17085444

    See all (35) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Asi1 regulates distribution of proteins at the nuclear membrane.
      Title: Distribution of Proteins at the Inner Nuclear Membrane Is Regulated by the Asi1 E3 Ligase in Saccharomyces cerevisiae.
    2. In dal81Delta mutants, the repressing activity of the Asi proteins is dispensable, demonstrating that without amplification, the levels of full-length Stp1 and Stp2 that escape cytoplasmic retention are insufficient to activate transcription.
      Title: Dal81 enhances Stp1- and Stp2-dependent transcription necessitating negative modulation by inner nuclear membrane protein Asi1 in Saccharomyces cerevisiae.
    3. Latent precursor forms of Stp1 and Stp2 inefficiently enter the nucleus where Asi1 restricts them from binding SPS sensor-regulated promoters, revealing an unanticipated role of inner nuclear membrane proteins in controlling gene expression
      Title: Asi1 is an inner nuclear membrane protein that restricts promoter access of two latent transcription factors.
    4. Asi1, Asi2, and Asi3 work together to maintain the latent properties of transcription factors Stp1 and Stp2
      Title: Inner nuclear membrane proteins Asi1, Asi2, and Asi3 function in concert to maintain the latent properties of transcription factors Stp1 and Stp2.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by SGD

    Function Evidence Code Pubs
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables transferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables ubiquitin protein ligase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables ubiquitin-protein transferase activity IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    enables ubiquitin-protein transferase activity IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    enables zinc ion binding RCA
    inferred from Reviewed Computational Analysis
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in cellular response to amino acid stimulus IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in proteasome-mediated ubiquitin-dependent protein catabolic process IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in protein ubiquitination IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in ubiquitin-dependent protein catabolic process IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in ubiquitin-dependent protein catabolic process IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    involved_in ubiquitin-dependent protein catabolic process IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    part_of Asi complex IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in membrane IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in nuclear inner membrane IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in nuclear inner membrane IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in nuclear periphery HDA PubMed 
    located_in nucleus IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

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    Preferred Names
    putative ubiquitin-protein ligase ASI1
    NP_013837.1
    • Subunit of the inner nuclear membrane Asi ubiquitin ligase complex; the Asi complex targets both misfolded proteins of the inner nuclear membrane-associated degradation (INMAD) pathway and regulators of sterol biosynthesis for ubiquitin-mediated degradation; acts with Asi2p and Asi3p to ensure the fidelity of SPS-sensor signaling by targeting latent unprocessed forms of Stp1p and Stp2p, maintaining the repressed state of gene expression in the absence of inducing amino acids

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_001145.3 Reference assembly

      Range
      505333..507207
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_001182619.1 → NP_013837.1  TPA: putative ubiquitin-protein ligase ASI1 [Saccharomyces cerevisiae S288C]

      See identical proteins and their annotated locations for NP_013837.1

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      D6VZU2, P54074
      UniProtKB/TrEMBL
      C7GU99, C8ZEX2, G2WKG9, N1NYT3
      Conserved Domains (1) summary
      cd16616
      Location:568 → 611
      mRING-HC-C4C4_Asi1p_like; Modified RING finger, HC subclass (C4C4-type), found in Saccharomyces cerevisiae amino acid sensor-independent protein Asi1p, Asi3p and similar proteins
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    P54074.1

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